Abstract
Previously, root colonization by the rhizobacterium, Pseudomonas chlororaphis O6, was shown to induce expression of galactinol synthase conferring systemic resistance against a fungal pathogen in cucumber leaves. Here, the Arabidopsis – Botrytis cinerea system is introduced to better understand signal transduction of galactinol and (or) raffinose family oligosaccharides (RFO) during O6-mediated induced systemic resistance (ISR). Among the 10 Arabidopsis galactinol synthase genes, only AtGolS1 was specifically induced upon infection with the fungal pathogen B. cinerea. AtGolS1 was primed by O6 colonization against the pathogen in Arabidopsis leaves. Arabidopsis T-DNA insertion mutants at the AtGolS1 gene site compromised O6-mediated ISR against the pathogen, thereby suggesting that AtGolS1 plays an important role in ISR. O6 colonization increased AtGolS1 transcription as well as ISR in several Arabidopsis signaling mutants, but not in the jar1-1 and coi1 mutant lines. Exogenous jasmonate treatment induced transcription of AtGolS1 in wild-type Col-0 plants, but salicylic acid and 1-aminocyclopropane-1-carboxylate did not. These studies on signaling mutants and target gene expression indicate that expression of AtGolS1 in response to O6 colonization is mediated through the jasmonate-dependent pathway, stimulating ISR in Arabidopsis against B. cinerea infection.
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Claims 9
Claim · model-written summary
AtGolS1 alone among ten galactinol synthase genes was induced upon Botrytis infection.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Interactor
- B. cinerea
Botrytis cinereae0pathogen - Gene
- AtGolS1e0
- Outcome
- AtGolS1 was specifically inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the 10 Arabidopsis galactinol synthase genes, only AtGolS1 was specifically induced upon infection with the fungal pathogen B. cinerea.”
t0“Arabidopsis thaliana”
Claim · model-written summary
O6 colonization primed AtGolS1 in Arabidopsis leaves against the pathogen.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- leavese0
- Treatment
- O6 colonizatione0
- Interactor
- O6e0symbiont
- Gene
- AtGolS1e0
- Outcome
- AtGolS1 was primede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtGolS1 was primed by O6 colonization against the pathogen in Arabidopsis leaves.”
Claim · model-written summary
AtGolS1 insertion mutants compromised O6-mediated systemic resistance.
- Taxon
- ArabidopsisArabidopsise0
- Material
- T-DNA insertion mutantse0
- Treatment
- T-DNA insertion mutantse0
- Interactor
- O6e0symbiont
- Gene
- AtGolS1e0
- Outcome
- O6-mediated ISRe0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis T-DNA insertion mutants at the AtGolS1 gene site compromised O6-mediated ISR against the pathogen”
Claim · model-written summary
O6 colonization increased AtGolS1 transcription in several signaling mutants, except jar1-1 and coi1.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- O6 colonizatione0
- Interactor
- O6e0symbiont
- Gene
- AtGolS1e0
- Outcome
- AtGolS1 transcriptione0
- Direction
- increasede0
- Qualifier
- but not in the jar1-1 and coi1 mutant linese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“O6 colonization increased AtGolS1 transcription as well as ISR in several Arabidopsis signaling mutants, but not in the jar1-1 and coi1 mutant lines.”
Claim · model-written summary
O6 colonization increased systemic resistance in several signaling mutants, except jar1-1 and coi1.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- O6 colonizatione0
- Interactor
- O6e0symbiont
- Outcome
- ISRe0
- Direction
- increasede0
- Qualifier
- but not in the jar1-1 and coi1 mutant linese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“O6 colonization increased AtGolS1 transcription as well as ISR in several Arabidopsis signaling mutants, but not in the jar1-1 and coi1 mutant lines.”
Claim · model-written summary
Exogenous jasmonate induced AtGolS1 transcription in wild-type Col-0 plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- wild-type Col-0e0
- Treatment
- Exogenous jasmonate treatmente0
- Gene
- AtGolS1e0
- Outcome
- transcription of AtGolS1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous jasmonate treatment induced transcription of AtGolS1 in wild-type Col-0 plants”
t0“Arabidopsis thaliana”
Claim · model-written summary
Salicylic acid did not induce AtGolS1 transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- salicylic acide0
- Gene
- AtGolS1e1
- Outcome
- transcription of AtGolS1e1
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but salicylic acid and 1-aminocyclopropane-1-carboxylate did not.”
t0“Jasmonate-dependent expression of a galactinol synthase gene is involved in priming of systemic fungal resistance in Arabidopsis thaliana”
e1“transcription of AtGolS1”
Claim · model-written summary
1-aminocyclopropane-1-carboxylate did not induce AtGolS1 transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- 1-aminocyclopropane-1-carboxylatee0
- Gene
- AtGolS1e1
- Outcome
- transcription of AtGolS1e1
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but salicylic acid and 1-aminocyclopropane-1-carboxylate did not.”
t0“Jasmonate-dependent expression of a galactinol synthase gene is involved in priming of systemic fungal resistance in Arabidopsis thaliana”
e1“transcription of AtGolS1”
Claim · model-written summary
AtGolS1 expression following O6 colonization is mediated through a jasmonate-dependent pathway that stimulates resistance.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Second interactor
- O6e0symbiont
- Gene
- AtGolS1e0
- Outcome
- ISRe0
- Qualifier
- jasmonate-dependent pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of AtGolS1 in response to O6 colonization is mediated through the jasmonate-dependent pathway, stimulating ISR in Arabidopsis against B. cinerea infection.”
The same questions in other papers
salicylic acid · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
11 papers in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- positive
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.