Abstract
Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress, is a member of the group II WRKY proteins and responds to both abiotic and biotic stress. Heat-treated seeds and plants of WRKY39 knock-down mutants had increased susceptibility to heat stress, showing reduced germination, decreased survival, and elevated electrolyte leakage compared with wild-type plants. In contrast, WRKY39 over-expressing plants exhibited enhanced thermotolerance compared with wild-type plants. RT-PCR and qRT-PCR analysis of wrky39 mutants and WRKY39 over-expressing plants identified putative genes regulated by WRKY39. Consistent with a role for WRKY39 in heat tolerance, the expression levels of salicylic acid (SA)-regulated PR1 and SA-related MBF1c genes were downregulated in wrky39 mutants. In contrast, over-expression of WRKY39 increased the expression of PR1 and MBF1c. The WRKY39 transcript was induced in response to treatment with SA or methyljasmonate. Analysis of heat stress-induced WRKY39 in defense signaling mutants, including coi1, ein2, and sid2, further indicated that WRKY39 was positively co-regulated by the SA and jasmonate (JA) signaling pathways. Together, these findings reveal that heat stress-induced WRKY39 positively regulates the cooperation between the SA- and JA-activated signaling pathways that mediate responses to heat stress.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Heat induces WRKY39 transcription factor.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- WRKY39e0
- Outcome
- transcription factor that is induced by heat stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress”
Claim · model-written summary
WRKY39 belongs to group II WRKY proteins and responds to abiotic and biotic stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- WRKY39e0
- Outcome
- responds to both abiotic and biotic stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress, is a member of the group II WRKY proteins and responds to both abiotic and biotic stress.”
Claim · model-written summary
Heat-treated WRKY39 knock-down mutants had lower germination and survival and higher electrolyte leakage than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- WRKY39 knock-down mutantse0
- Treatment
- Heat-treatede0
- Outcome
- increased susceptibility to heat stresse0
- Direction
- increasede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat-treated seeds and plants of WRKY39 knock-down mutants had increased susceptibility to heat stress, showing reduced germination, decreased survival, and elevated electrolyte leakage compared with wild-type plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
WRKY39 overexpression enhanced thermotolerance relative to wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- WRKY39 over-expressinge0
- Outcome
- thermotolerancee0
- Direction
- enhancede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY39 over-expressing plants exhibited enhanced thermotolerance compared with wild-type plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Expression analysis identified putative genes regulated by WRKY39.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- WRKY39e0
- Outcome
- putative genes regulated by WRKY39e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RT-PCR and qRT-PCR analysis of wrky39 mutants and WRKY39 over-expressing plants identified putative genes regulated by WRKY39.”
t0“Arabidopsis thaliana”
Claim · model-written summary
PR1 and MBF1c expression was downregulated in wrky39 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- wrky39 mutantse0
- Gene
- PR1 and SA-related MBF1c genese0
- Outcome
- expression levelse0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of salicylic acid (SA)-regulated PR1 and SA-related MBF1c genes were downregulated in wrky39 mutants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
WRKY39 overexpression increased PR1 and MBF1c expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- over-expression of WRKY39e0
- Gene
- PR1 and MBF1ce0
- Outcome
- expression of PR1 and MBF1ce0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of WRKY39 increased the expression of PR1 and MBF1c.”
t0“Arabidopsis thaliana”
Claim · model-written summary
SA or methyljasmonate treatment induced WRKY39 transcript.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- SA or methyljasmonatee0
- Gene
- WRKY39e0
- Outcome
- WRKY39 transcripte0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The WRKY39 transcript was induced in response to treatment with SA or methyljasmonate.”
t0“Arabidopsis thaliana”
Claim · model-written summary
SA and JA signaling pathways positively co-regulated heat-induced WRKY39.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- WRKY39e0
- Outcome
- WRKY39 was positively co-regulatede0
- Qualifier
- SA and jasmonate (JA) signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY39 was positively co-regulated by the SA and jasmonate (JA) signaling pathways.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Authors conclude heat-induced WRKY39 positively regulates cooperation between SA and JA signaling in heat responses.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- WRKY39e0
- Outcome
- cooperation between the SA- and JA-activated signaling pathwayse0
- Qualifier
- responses to heat stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heat stress-induced WRKY39 positively regulates the cooperation between the SA- and JA-activated signaling pathways that mediate responses to heat stress.”
t0“Arabidopsis thaliana”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- no effect
- no effect
7 papers more in this group
See all 11 papers →salicylic acid · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
15 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- no effect
- no effect
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.