Abstract
Abstract In silico analysis of the promoter region of the At4g01870 gene of Arabidopsis thaliana (L.) Heynh. showed the presence of ABRE, W-box, RAV1-A, MYB, and LFY cis-elements in the sequence. These regulatory motifs bind the transcription factors involved in responses to abscisic acid (ABA) and stresses. Stable transgenic plants carrying the β-glucuronidase gene under the control of the 5'-deletion fragments of the At4g01870 promoter were obtained. According to the results of histochemical staining of transformants, gene expression was induced by abiotic stress and was most significant in the conductive tissues of the root, leaves, and sepals as well as in flowers. The study of At4g01870 gene expression by RT-PCR confirmed that the gene transcript content increased after the exposure of plants to a solution of NaCl or at 37°C and after ABA treatment; however, hypothermia almost unchanged the level of accumulation of the transcripts. Along with ABA, expression of the At4g01870 gene was induced by indolylacetic and salicylic acids and ethylene precursor 1-aminocyclopropane-1-carboxylic acid; it was hardly regulated by methyl jasmonate and inhibited by cytokinin. The TolB-like protein, encoded by the At4g01870 gene, functions as a type of platform, based on which protein complexes are assembled. Given the previously identified ABA-binding properties of the protein At4g01870 and the presence of the ABA-dependent cis-elements in the promoter of its coding gene, it can be assumed that the protein encoded by the At4g01870 gene allows to control the hormonal signals in the cell, providing a structural platform for the interaction of specific effector proteins, trans-factors and ion channels.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
The At4g01870 promoter contains ABRE, W-box, RAV1-A, MYB and LFY motifs.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- At4g01870e0
- Outcome
- presence of ABRE, W-box, RAV1-A, MYB, and LFY cis-elementse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter region of the At4g01870 gene of Arabidopsis thaliana (L.) Heynh. showed the presence of ABRE, W-box, RAV1-A, MYB, and LFY cis-elements”
Claim · model-written summary
Abiotic stress induced reporter expression, especially in conductive root tissues, leaves, sepals and flowers.
- Taxon
- ArabidopsisthalianaAt4g0180t0
- Organ / stage
- conductive tissues of the root, leaves, and sepals as well as in flowerse0
- Treatment
- abiotic stresse0
- Outcome
- gene expressione0
- Direction
- inducede0
Not reportedComparator
Verbatim
t0“ArabidopsisthalianaAt4g0180”
e0“gene expression was induced by abiotic stress and was most significant in the conductive tissues of the root, leaves, and sepals as well as in flowers”
Claim · model-written summary
At4g01870 transcript content increased after NaCl exposure, heat at 37°C, and ABA treatment.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- a solution of NaCl or at 37°C and after ABA treatmente0
- Gene
- At4g01870e0
- Outcome
- gene transcript contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At4g01870 gene expression by RT-PCR confirmed that the gene transcript content increased after the exposure of plants to a solution of NaCl or at 37°C and after ABA treatment”
e1“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Hypothermia left At4g01870 transcript accumulation almost unchanged.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- hypothermiae0
- Gene
- At4g01870e1
- Outcome
- level of accumulation of the transcriptse0
- Direction
- almost unchangede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hypothermia almost unchanged the level of accumulation of the transcripts”
e1“At4g01870 gene expression”
e2“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Indolylacetic acid induced At4g01870 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- indolylacetice0
- Outcome
- expression of the At4g01870 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the At4g01870 gene was induced by indolylacetic and salicylic acids and ethylene precursor 1-aminocyclopropane-1-carboxylic acid”
e1“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Salicylic acid induced At4g01870 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- salicylic acidse0
- Outcome
- expression of the At4g01870 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the At4g01870 gene was induced by indolylacetic and salicylic acids and ethylene precursor 1-aminocyclopropane-1-carboxylic acid”
e1“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
The ethylene precursor 1-aminocyclopropane-1-carboxylic acid induced At4g01870 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- 1-aminocyclopropane-1-carboxylic acide0
- Outcome
- expression of the At4g01870 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the At4g01870 gene was induced by indolylacetic and salicylic acids and ethylene precursor 1-aminocyclopropane-1-carboxylic acid”
e1“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Methyl jasmonate hardly regulated At4g01870 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- methyl jasmonatee0
- Outcome
- expression of the At4g01870 genee1
- Direction
- hardly regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was hardly regulated by methyl jasmonate”
e1“expression of the At4g01870 gene”
e2“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Cytokinin inhibited At4g01870 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- cytokinine0
- Outcome
- expression of the At4g01870 genee1
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibited by cytokinin”
e1“expression of the At4g01870 gene”
e2“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
The authors propose that At4g01870 protein serves as a platform for hormonal signal control.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- At4g01870e0
- Outcome
- control the hormonal signals in the celle0
- Qualifier
- providing a structural platform for the interaction of specific effector proteins, trans-factors and ion channelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it can be assumed that the protein encoded by the At4g01870 gene allows to control the hormonal signals in the cell, providing a structural platform for the interaction of specific effector proteins, trans-factors and ion channels”
e1“Arabidopsis thaliana (L.) Heynh.”
The same questions in other papers
1-aminocyclopropanecarboxylic acid · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
6 papers more in this group
See all 10 papers →(-)-methyl jasmonate · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →salicylic acid · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
15 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- no effect
- no effect
24 papers more in this group
See all 28 papers →(+)-abscisic acid · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
30 papers in the same direction as this paper · 19 in the opposite direction
- negative
- no effect
- negative
46 papers more in this group
See all 50 papers →class · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
19 papers in the same direction as this paper · 23 in the opposite direction
- positive
- positive
- positive
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.