Abstract
BACKGROUND: Banana fruit is highly susceptible to chilling injury. In previous research it was shown that heat pretreatment of banana fruit at 38 °C for 3 days before storage at a chilling temperature of 8 °C for 12 days prevented increases in visible chilling injury index, electrolyte leakage and malondialdehyde content and also decreases in lightness and chroma, indicating that heat pretreatment could effectively alleviate chilling injury of banana fruit. However, little is known about the role of small heat shock proteins (sHSPs) in postharvest chilling tolerance of banana fruit. In the present study, three cytosolic sHSP expression profiles in peel and pulp tissues of banana fruit during heat pretreatment and subsequent chilled storage (8 °C) were investigated in relation to heat pretreatment-induced chilling tolerance. RESULTS: Three full-length cDNAs of cytosolic sHSP genes, including two class I sHSP (CI sHSP) and one class II sHSP (CII sHSP) cDNAs, named Ma-CI sHSP1, Ma-CI sHSP2 and Ma-CII sHSP3 respectively, were isolated and characterised from harvested banana fruit. Accumulation of Ma-CI sHSP1 mRNA transcripts in peel and pulp tissues and Ma-CII sHSP3 mRNA transcripts in peel tissue increased during heat pretreatment. Expression of all three Ma-sHSP genes in peel and pulp tissues was induced during subsequent chilled storage. Furthermore, Ma-CI sHSP1 and Ma-CII sHSP3 mRNA transcripts in pulp tissue and Ma-CI sHSP2 mRNA transcripts in peel and pulp tissues were obviously enhanced by heat pretreatment at days 6 and 9 of subsequent chilled storage. CONCLUSION: These results suggested that heat pretreatment enhanced the expression of Ma-sHSPs, which might be involved in heat pretreatment-induced chilling tolerance of banana fruit.
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Claims 6
Claim · model-written summary
Three cytosolic sHSP cDNAs were isolated and characterized from banana fruit.
Verbatim
e0“Three full-length cDNAs of cytosolic sHSP genes, including two class I sHSP (CI sHSP) and one class II sHSP (CII sHSP) cDNAs, named Ma-CI sHSP1, Ma-CI sHSP2 and Ma-CII sHSP3 respectively, were isolated and characterised from harvested banana fruit”
Claim · model-written summary
Heat pretreatment increased Ma-CI sHSP1 transcripts in peel and pulp.
- Taxon
- banana fruitt0
- Organ / stage
- peel and pulp tissuese0
- Treatment
- heat pretreatmente0
- Gene
- Ma-CI sHSP1e0
- Outcome
- Ma-CI sHSP1 mRNA transcriptse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Accumulation of Ma-CI sHSP1 mRNA transcripts in peel and pulp tissues and Ma-CII sHSP3 mRNA transcripts in peel tissue increased during heat pretreatment”
t0“banana fruit”
Claim · model-written summary
Heat pretreatment increased Ma-CII sHSP3 transcripts in peel.
- Taxon
- banana fruitt0
- Organ / stage
- peel tissuee0
- Treatment
- heat pretreatmente0
- Gene
- Ma-CII sHSP3e0
- Outcome
- Ma-CII sHSP3 mRNA transcriptse0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Ma-CII sHSP3 mRNA transcripts in peel tissue increased during heat pretreatment”
t0“banana fruit”
Claim · model-written summary
Chilled storage induced all three Ma-sHSP genes in peel and pulp.
- Taxon
- banana fruitt0
- Organ / stage
- peel and pulp tissuese0
- Treatment
- subsequent chilled storagee0
- Outcome
- Expression of all three Ma-sHSP genese0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“Expression of all three Ma-sHSP genes in peel and pulp tissues was induced during subsequent chilled storage”
t0“banana fruit”
Claim · model-written summary
Heat pretreatment enhanced Ma-CI sHSP1 and Ma-CII sHSP3 transcripts in pulp on days 6 and 9 of chilling.
- Taxon
- banana fruitt0
- Organ / stage
- pulp tissuee0
- Treatment
- heat pretreatmente0
- Outcome
- Ma-CI sHSP1 and Ma-CII sHSP3 mRNA transcriptse0
- Direction
- enhancede0
- Qualifier
- days 6 and 9 of subsequent chilled storagee0
Not reportedComparator
Verbatim
e0“Ma-CI sHSP1 and Ma-CII sHSP3 mRNA transcripts in pulp tissue and Ma-CI sHSP2 mRNA transcripts in peel and pulp tissues were obviously enhanced by heat pretreatment at days 6 and 9 of subsequent chilled storage”
t0“banana fruit”
Claim · model-written summary
Heat pretreatment enhanced Ma-CI sHSP2 transcripts in peel and pulp on days 6 and 9 of chilling.
- Taxon
- banana fruitt0
- Organ / stage
- peel and pulp tissuese0
- Treatment
- heat pretreatmente0
- Outcome
- Ma-CI sHSP2 mRNA transcriptse0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Ma-CI sHSP2 mRNA transcripts in peel and pulp tissues were obviously enhanced by heat pretreatment at days 6 and 9 of subsequent chilled storage”
t0“banana fruit”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.