Abstract
Many evidences have indicated that temperature stress can induce heat shock protein expression and heat treatment can alleviate chilling injury of fruits.In the present study,two HSP70 fragments were cloned from banana peel using homology-based method in order to investigate their expressions regulated by temperature stress and the relationship between their expressions and chilling injury.Using PCR degenerate primers designed with reference to the conserved amino acid sequences of known HSP70,total RNA extracted from banana peel was used as templates and HSP70 cDNA fragments were amplified by RT-PCR in order to study their expression characteristics under different environments by Northern hybridization.Two different cDNA fragments,named Ma-HSP70-1 and Ma-HSP70-2,were cloned.The homology between two HSP70 is 86.9% at nucleotide acid sequence level and 97.1% at amino acid sequence level.Using Ma-HSP70-1 and Ma-HSP70-2 as probes,Northern hybridization showed that short-time heat pretreatment(38 ℃)only induced the expression of Ma-HSP70-2 transcripts,However,storage at low temperature enhanced accumulations of 2 HSP70 mRNA.Ma-HSP70 might be related to chilling tolerance of banana fruits.
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Claims 6
Claim · model-written summary
Two distinct banana peel HSP70 cDNA fragments, Ma-HSP70-1 and Ma-HSP70-2, were cloned.
Verbatim
e0“Two different cDNA fragments,named Ma-HSP70-1 and Ma-HSP70-2,were cloned”
t0“banana”
Claim · model-written summary
The two banana HSP70 fragments have 86.9% nucleotide sequence homology.
Verbatim
e0“The homology between two HSP70 is 86.9% at nucleotide acid sequence level and 97.1% at amino acid sequence level”
t0“banana”
Claim · model-written summary
The two banana HSP70 fragments have 97.1% amino acid sequence homology.
Verbatim
e0“The homology between two HSP70 is 86.9% at nucleotide acid sequence level and 97.1% at amino acid sequence level”
t0“banana”
Claim · model-written summary
Short heat pretreatment at 38 ℃ induced only Ma-HSP70-2 transcripts.
- Taxon
- bananat0
- Treatment
- short-time heat pretreatment(38 ℃)e0
- Gene
- Ma-HSP70-2e0
- Outcome
- expression of Ma-HSP70-2 transcriptse0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“short-time heat pretreatment(38 ℃)only induced the expression of Ma-HSP70-2 transcripts”
t0“banana”
Claim · model-written summary
Low-temperature storage enhanced accumulation of both HSP70 mRNAs in banana peel.
- Taxon
- bananat0
- Treatment
- storage at low temperaturee0
- Gene
- HSP70e0
- Outcome
- accumulations of 2 HSP70 mRNAe0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“storage at low temperature enhanced accumulations of 2 HSP70 mRNA”
t0“banana”
Claim · model-written summary
Authors suggest Ma-HSP70 may be related to banana fruit chilling tolerance.
Not reportedDirection
Verbatim
e0“Ma-HSP70 might be related to chilling tolerance of banana fruits”
The same questions in other papers
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See all 6 papers →storage · molecular: expression · MusaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.