Abstract
Cold stress adversely affects plant production, both qualitatively and quantitatively. Banana (Musa acuminata) is sensitive to cold stress and suffers chilling injury (CI) when stored under 11°C, causing abnormal fruit softening. However, the mechanism underlying the abnormal fruit softening due to CI remains obscure. This study uncovered the coordinated transcriptional mechanism of ethylene F-box (EBF1) protein and abscisic acid-insensitive 5 (ABI5)-like protein in regulating chilling-induced softening disorders of Fenjiao banana. Cold stress severely inhibited the transcript and protein levels of EBF1, ABI5-like, and fruit softening-related genes. The ABI5-like protein bound to the promoters of key starch and cell wall degradation-related genes such as β-amylase 8 (BAM8), pectate lyase 8 (PL8), and β-D-xylosidase23-like (XYL23-like) and activated their activities. EBF1 physically interacted with ABI5-like and enhanced the transcriptional activity of the key starch and cell wall degradation-related genes but did not ubiquitinate or degrade ABI5-like protein. This promoted fruit ripening and ameliorated fruit CI in a manner similar to the effect of exogenous abscisic acid treatment. The ectopic and transient overexpression of EBF1 and ABI5-like genes in tomato (Solanum lycopersicum) and Fenjiao banana accelerated fruit ripening and softening by promoting ethylene production, starch and cell wall degradation, and decreasing fruit firmness. EBF1 interacted with EIL4 but did not ubiquitinate or degrade EIL4, which is inconsistent with the typical role of EBF1/2 in Arabidopsis (Arabidopsis thaliana). These results collectively highlight that the interaction of EBF1 and ABI5-like controls starch and cell wall metabolism in banana, which is strongly inhibited by chilling stress, leading to fruit softening and ripening disorder.
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Claims 12
Claim · model-written summary
Storage below 11°C caused chilling injury and abnormal banana fruit softening.
Verbatim
e0“Banana (Musa acuminata) is sensitive to cold stress and suffers chilling injury (CI) when stored under 11°C, causing abnormal fruit softening.”
Claim · model-written summary
Cold stress inhibited EBF1, ABI5-like, and fruit softening gene transcript and protein levels.
- Taxon
- bananae1
- Treatment
- Cold stresse0
- Outcome
- transcript and protein levels of EBF1, ABI5-like, and fruit softening-related genese0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Cold stress severely inhibited the transcript and protein levels of EBF1, ABI5-like, and fruit softening-related genes.”
e1“softening disorders of Fenjiao banana”
Claim · model-written summary
ABI5-like bound promoters of BAM8, PL8, and XYL23-like and activated them.
Verbatim
e0“The ABI5-like protein bound to the promoters of key starch and cell wall degradation-related genes such as β-amylase 8 (BAM8), pectate lyase 8 (PL8), and β-D-xylosidase23-like (XYL23-like) and activated their activities.”
e1“softening disorders of Fenjiao banana”
Claim · model-written summary
EBF1 physically interacted with ABI5-like.
Verbatim
e0“EBF1 physically interacted with ABI5-like”
e1“softening disorders of Fenjiao banana”
Claim · model-written summary
EBF1 enhanced transcriptional activity of starch and cell wall degradation genes.
Verbatim
e0“EBF1 physically interacted with ABI5-like and enhanced the transcriptional activity of the key starch and cell wall degradation-related genes”
e1“softening disorders of Fenjiao banana”
Claim · model-written summary
EBF1 did not ubiquitinate or degrade ABI5-like.
Verbatim
e0“did not ubiquitinate or degrade ABI5-like protein”
e1“softening disorders of Fenjiao banana”
Claim · model-written summary
The EBF1 and ABI5-like interaction promoted fruit ripening and ameliorated chilling injury.
Verbatim
e0“This promoted fruit ripening and ameliorated fruit CI”
e1“EBF1 physically interacted with ABI5-like”
e2“softening disorders of Fenjiao banana”
Claim · model-written summary
EBF1 and ABI5-like overexpression accelerated fruit ripening and softening in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- overexpression of EBF1 and ABI5-like genese0
- Outcome
- fruit ripening and softeninge0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ectopic and transient overexpression of EBF1 and ABI5-like genes in tomato (Solanum lycopersicum) and Fenjiao banana accelerated fruit ripening and softening”
Claim · model-written summary
EBF1 and ABI5-like overexpression accelerated fruit ripening and softening in Fenjiao banana.
- Taxon
- Fenjiao bananae0
- Treatment
- overexpression of EBF1 and ABI5-like genese0
- Outcome
- fruit ripening and softeninge0
- Direction
- acceleratede0
Not reportedComparator
Verbatim
e0“The ectopic and transient overexpression of EBF1 and ABI5-like genes in tomato (Solanum lycopersicum) and Fenjiao banana accelerated fruit ripening and softening”
Claim · model-written summary
Overexpression promoted ethylene production and starch and cell wall degradation.
- Taxon
- Fenjiao bananae0
- Treatment
- overexpression of EBF1 and ABI5-like genese0
- Outcome
- ethylene production, starch and cell wall degradatione0
- Direction
- promotinge0
Not reportedComparator
Verbatim
e0“overexpression of EBF1 and ABI5-like genes in tomato (Solanum lycopersicum) and Fenjiao banana accelerated fruit ripening and softening by promoting ethylene production, starch and cell wall degradation”
Claim · model-written summary
Overexpression decreased fruit firmness.
- Taxon
- Fenjiao bananae0
- Treatment
- overexpression of EBF1 and ABI5-like genese0
- Outcome
- fruit firmnesse0
- Direction
- decreasinge0
Not reportedComparator
Verbatim
e0“overexpression of EBF1 and ABI5-like genes in tomato (Solanum lycopersicum) and Fenjiao banana accelerated fruit ripening and softening by promoting ethylene production, starch and cell wall degradation, and decreasing fruit firmness”
Claim · model-written summary
EBF1 interacted with EIL4 without ubiquitinating or degrading it.
Verbatim
e0“EBF1 interacted with EIL4 but did not ubiquitinate or degrade EIL4”
e1“softening disorders of Fenjiao banana”
The same questions in other papers
cold · molecular: expression · MusaPositive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.