Abstract
The isoflavonoid conjugates medicarpin-3-O-glucoside-6''-O-malonate (MGM), afrormosin-7-O-glucoside (AG), and afrormosin-7-O-glucoside-6''-O-malonate (AGM) were isolated and characterized from cell suspension cultures of alfalfa (Medicago sativa L.), where they were the major constitutive secondary metabolites. They were also found in alfalfa roots but not in other parts of the plant. The phytoalexin medicarpin accumulated rapidly in suspension cultured cells treated with elicitor from Colletotrichum lindemuthianum, and this was subsequently accompanied by an increase in the levels of MGM. In contrast, net accumulation of afrormosin conjugates was not affected by elicitor treatment. Labeling studies with [(14)C]phenylalanine indicated that afrormosin conjugates were the major de novo synthesized isoflavonoid products in unelicited cells. During elicitation, [(14)C]phenylalanine was incorporated predominantly into medicarpin, although a significant proportion of the newly synthesized medicarpin was also conjugated. Treatment of (14)C-labeled, elicited cells with l-alpha-aminooxy-beta-phenylpropionic acid, a potent inhibitor of PAL activity in vivo, resulted in the initial appearance of labeled medicarpin of very low specific activity, suggesting that the phytoalexin could be released from a preformed conjugate under these conditions. Our data draw attention to the involvement of isoflavone hydroxylases during the constitutive and elicitor-induced accumulation of isoflavonoids and their conjugates in alfalfa cell cultures.
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Claims 9
Claim · model-written summary
The isoflavonoid conjugates MGM, AG and AGM were isolated from alfalfa cell suspension cultures, where they were the major constitutive secondary metabolites.
- Taxon
- alfalfa
Medicago sativae0 - Organ / stage
- cell suspension culturese0
- Compound
- medicarpin-3-O-glucoside-6''-O-malonate (MGM)e0
- Outcome
- major constitutive secondary metabolitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isoflavonoid conjugates medicarpin-3-O-glucoside-6''-O-malonate (MGM), afrormosin-7-O-glucoside (AG), and afrormosin-7-O-glucoside-6''-O-malonate (AGM) were isolated and characterized from cell suspension cultures of alfalfa (Medicago sativa L.), where they were the major constitutive secondary metabolites”
t0“Alfalfa”
Claim · model-written summary
The conjugates were also found in alfalfa roots but not in other parts of the plant.
- Taxon
- alfalfaMedicago sativae0
- Organ / stage
- rootse0
- Outcome
- found in alfalfa roots but not in other parts of the plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They were also found in alfalfa roots but not in other parts of the plant”
t0“Alfalfa”
Claim · model-written summary
Medicarpin accumulated rapidly in alfalfa suspension cultured cells treated with elicitor from Colletotrichum lindemuthianum.
- Taxon
- AlfalfaMedicago sativat0
- Organ / stage
- suspension cultured cellse0
- Treatment
- elicitor from Colletotrichum lindemuthianume0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Outcome
- medicarpine0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phytoalexin medicarpin accumulated rapidly in suspension cultured cells treated with elicitor from Colletotrichum lindemuthianum, and this was subsequently accompanied by an increase in the levels of MGM”
t0“Alfalfa”
Claim · model-written summary
Levels of medicarpin-3-O-glucoside-6''-O-malonate (MGM) increased after elicitor treatment of alfalfa cells, following medicarpin accumulation.
- Taxon
- AlfalfaMedicago sativat0
- Organ / stage
- suspension cultured cellse0
- Treatment
- elicitor from Colletotrichum lindemuthianume0
- Interactor
- Colletotrichum lindemuthianumColletotrichum lindemuthianume0pathogen
- Outcome
- levels of MGMe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phytoalexin medicarpin accumulated rapidly in suspension cultured cells treated with elicitor from Colletotrichum lindemuthianum, and this was subsequently accompanied by an increase in the levels of MGM”
t0“Alfalfa”
Claim · model-written summary
Net accumulation of afrormosin conjugates in alfalfa cells was not affected by elicitor treatment.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- elicitor treatmente0
- Outcome
- net accumulation of afrormosin conjugatese0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“net accumulation of afrormosin conjugates was not affected by elicitor treatment”
t0“Alfalfa”
Claim · model-written summary
Labeling with [14C]phenylalanine indicated that afrormosin conjugates were the major de novo synthesized isoflavonoid products in unelicited alfalfa cells.
- Taxon
- AlfalfaMedicago sativat0
- Compound
- afrormosin conjugatese0
- Outcome
- major de novo synthesized isoflavonoid productse0
- Qualifier
- unelicited cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Labeling studies with [(14)C]phenylalanine indicated that afrormosin conjugates were the major de novo synthesized isoflavonoid products in unelicited cells”
t0“Alfalfa”
Claim · model-written summary
During elicitation [14C]phenylalanine was incorporated predominantly into medicarpin, and a significant proportion of the newly synthesized medicarpin was also conjugated.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- elicitatione0
- Compound
- medicarpine0
- Outcome
- incorporated predominantly into medicarpine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During elicitation, [(14)C]phenylalanine was incorporated predominantly into medicarpin, although a significant proportion of the newly synthesized medicarpin was also conjugated”
t0“Alfalfa”
Claim · model-written summary
Treating 14C-labeled elicited alfalfa cells with the PAL inhibitor l-alpha-aminooxy-beta-phenylpropionic acid resulted in initial appearance of labeled medicarpin of very low specific activity, suggesting release from a preformed conjugate.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- l-alpha-aminooxy-beta-phenylpropionic acide0
- Outcome
- labeled medicarpin of very low specific activitye0
- Direction
- appearancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of (14)C-labeled, elicited cells with l-alpha-aminooxy-beta-phenylpropionic acid, a potent inhibitor of PAL activity in vivo, resulted in the initial appearance of labeled medicarpin of very low specific activity”
t0“Alfalfa”
Claim · model-written summary
The authors conclude that isoflavone hydroxylases are involved in constitutive and elicitor-induced accumulation of isoflavonoids and their conjugates in alfalfa cell cultures.
- Taxon
- alfalfaMedicago sativae0
- Gene
- isoflavone hydroxylasese0
- Outcome
- accumulation of isoflavonoids and their conjugatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our data draw attention to the involvement of isoflavone hydroxylases during the constitutive and elicitor-induced accumulation of isoflavonoids and their conjugates in alfalfa cell cultures”
The same questions in other papers
resistance inducer · molecular: metabolites · Medicago sativa (alfalfa)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.