Abstract
In eukaryotes, 45S rRNA genes are arranged in tandem arrays in copy numbers ranging from several hundred to several thousand in plants. Although it is clear that not all copies are transcribed under normal growth conditions, the molecular basis controlling the expression of specific sets of rRNA genes remains unclear. Here, we report four major rRNA gene variants in Arabidopsis thaliana. Interestingly, while transcription of one of these rRNA variants is induced, the others are either repressed or remain unaltered in A. thaliana plants with a disrupted nucleolin-like protein gene (Atnuc-L1). Remarkably, the most highly represented rRNA gene variant, which is inactive in WT plants, is reactivated in Atnuc-L1 mutants. We show that accumulated pre-rRNAs originate from RNA Pol I transcription and are processed accurately. Moreover, we show that disruption of the AtNUC-L1 gene induces loss of symmetrical DNA methylation without affecting histone epigenetic marks at rRNA genes. Collectively, these data reveal a novel mechanism for rRNA gene transcriptional regulation in which the nucleolin protein plays a major role in controlling active and repressed rRNA gene variants in Arabidopsis.
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Claims 9
Claim · model-written summary
Four major rRNA gene variants occur in Arabidopsis thaliana
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Outcome
- rRNA gene variantse0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e0“we report four major rRNA gene variants in Arabidopsis thaliana”
Claim · model-written summary
Transcription of one rRNA gene variant is induced in plants with a disrupted nucleolin-like protein gene
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- disrupted nucleolin-like protein gene (Atnuc-L1)e1
- Gene
- Atnuc-L1e1
- Outcome
- transcription of one of these rRNA variantse1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e1“transcription of one of these rRNA variants is induced, the others are either repressed or remain unaltered in A. thaliana plants with a disrupted nucleolin-like protein gene (Atnuc-L1)”
Claim · model-written summary
Transcription of the other rRNA gene variants is repressed in plants with a disrupted nucleolin-like protein gene
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- disrupted nucleolin-like protein gene (Atnuc-L1)e1
- Gene
- Atnuc-L1e1
- Outcome
- the otherse1
- Direction
- repressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e1“transcription of one of these rRNA variants is induced, the others are either repressed or remain unaltered in A. thaliana plants with a disrupted nucleolin-like protein gene (Atnuc-L1)”
Claim · model-written summary
Other rRNA gene variants remain unaltered in plants with a disrupted nucleolin-like protein gene
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- disrupted nucleolin-like protein gene (Atnuc-L1)e1
- Gene
- Atnuc-L1e1
- Outcome
- the otherse1
- Direction
- remain unalterede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e1“transcription of one of these rRNA variants is induced, the others are either repressed or remain unaltered in A. thaliana plants with a disrupted nucleolin-like protein gene (Atnuc-L1)”
Claim · model-written summary
The most highly represented rRNA gene variant, inactive in WT plants, is reactivated in Atnuc-L1 mutants
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Atnuc-L1 mutantse2
- Outcome
- the most highly represented rRNA gene variante2
- Direction
- reactivatede2
- Comparator
- WT plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e2“the most highly represented rRNA gene variant, which is inactive in WT plants, is reactivated in Atnuc-L1 mutants”
Claim · model-written summary
Accumulated pre-rRNAs originate from RNA Pol I transcription and are processed accurately
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- accumulated pre-rRNAse3
- Qualifier
- originate from RNA Pol I transcription and are processed accuratelye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e3“accumulated pre-rRNAs originate from RNA Pol I transcription and are processed accurately”
Claim · model-written summary
Disruption of AtNUC-L1 induces loss of symmetrical DNA methylation
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- disruption of the AtNUC-L1 genee4
- Gene
- AtNUC-L1e4
- Outcome
- symmetrical DNA methylatione4
- Direction
- losse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e4“disruption of the AtNUC-L1 gene induces loss of symmetrical DNA methylation without affecting histone epigenetic marks at rRNA genes”
Claim · model-written summary
Disruption of AtNUC-L1 does not affect histone epigenetic marks at rRNA genes
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- disruption of the AtNUC-L1 genee4
- Gene
- AtNUC-L1e4
- Outcome
- histone epigenetic marks at rRNA genese4
- Direction
- without affectinge4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e4“disruption of the AtNUC-L1 gene induces loss of symmetrical DNA methylation without affecting histone epigenetic marks at rRNA genes”
Claim · model-written summary
Nucleolin protein plays a major role in controlling active and repressed rRNA gene variants, a novel regulatory mechanism
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- nucleolin proteine5
- Outcome
- active and repressed rRNA gene variantse5
- Qualifier
- plays a major role in controllinge5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in Arabidopsis thaliana”
e5“the nucleolin protein plays a major role in controlling active and repressed rRNA gene variants in Arabidopsis”
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