Abstract
The effect of auxin (indole-3-acetic acid; IAA) on growth and incorporation of myo-[2-(3)H(N)]inositol ([(3)H]Ins) into noncellulosic polysaccharides in the cell walls of third internode sections from red light-grown pea seedlings (Pisum sativum L. cv. Alaska) was investigated. Intact sections were incubated on [(3)H]Ins for 4 hr to permit uptake of the tracer and then IAA was added. Growth started after a lag phase of 15 min under these conditions. The sections were removed from the tracer and separated into epidermis and cortical cylinder (cortex plus vascular tissue). In the epidermis, IAA-induced stimulation of [(3)H]Ins incorporation started after a lag of 15 min. The amount of incorporation was 15% higher after 30 min and 24% higher after 2 hr than in the control. In the cortical cylinder, IAA-induced stimulation of [(3)H]Ins incorporation started only approximately 1 hr after adding IAA. The ionophore monensin (20 muM) inhibited the IAA-induced growth by 95%. Under these conditions, the IAA-induced stimulation of [(3)H]Ins incorporation and the IAA-induced increase in in vivo extensibility of the sections was almost completely inhibited, although oxygen uptake was unaffected. We suggest that wall synthesis (as represented by [(3)H]Ins incorporation) and wall loosening (increase in in vivo extensibility) are related processes. The results support the hypothesis that IAA induces growth by rapid stimulation of cell wall synthesis in the growth-limiting epidermal cell layer.
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Claims 10
Claim · model-written summary
In pea epidermis, IAA-induced [(3)H]Ins incorporation was 15% higher than in the control after 30 min.
- Taxon
- pea
Lathyrus oleraceuse1 - Organ / stage
- epidermise2
- Treatment
- IAAe2
- Outcome
- [(3)H]Ins incorporatione2
- Direction
- highere3
- Value
- 15%e3
- Qualifier
- after 30 mine3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“In the epidermis, IAA-induced stimulation of [(3)H]Ins incorporation started after a lag of 15 min”
e3“The amount of incorporation was 15% higher after 30 min and 24% higher after 2 hr than in the control”
Claim · model-written summary
In pea epidermis, IAA-induced [(3)H]Ins incorporation was 24% higher than in the control after 2 hr.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- epidermise2
- Treatment
- IAAe2
- Outcome
- [(3)H]Ins incorporatione2
- Direction
- highere3
- Value
- 24%e3
- Qualifier
- after 2 hre3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“In the epidermis, IAA-induced stimulation of [(3)H]Ins incorporation started after a lag of 15 min”
e3“The amount of incorporation was 15% higher after 30 min and 24% higher after 2 hr than in the control”
Claim · model-written summary
In pea epidermis, IAA-induced stimulation of [(3)H]Ins incorporation started after a lag of 15 min.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- epidermise2
- Treatment
- IAAe2
- Outcome
- IAA-induced stimulation of [(3)H]Ins incorporatione2
- Value
- 15 mine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“In the epidermis, IAA-induced stimulation of [(3)H]Ins incorporation started after a lag of 15 min”
Claim · model-written summary
In the pea cortical cylinder, IAA-induced stimulation of [(3)H]Ins incorporation started only approximately 1 hr after adding IAA.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- cortical cylindere2
- Treatment
- IAAe2
- Outcome
- IAA-induced stimulation of [(3)H]Ins incorporatione2
- Value
- approximately 1 hre2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“In the cortical cylinder, IAA-induced stimulation of [(3)H]Ins incorporation started only approximately 1 hr after adding IAA”
Claim · model-written summary
Growth of pea internode sections started after a lag phase of 15 min.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- Growthe2
- Value
- 15 mine2
- Qualifier
- lag phasee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“Growth started after a lag phase of 15 min under these conditions”
Claim · model-written summary
The ionophore monensin (20 muM) inhibited IAA-induced growth of pea internode sections by 95%.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- ionophore monensin (20 muM)e2
- Outcome
- IAA-induced growthe2
- Direction
- inhibitede2
- Value
- 95%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“The ionophore monensin (20 muM) inhibited the IAA-induced growth by 95%”
Claim · model-written summary
Monensin almost completely inhibited the IAA-induced stimulation of [(3)H]Ins incorporation and the IAA-induced increase in in vivo extensibility of pea sections.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- ionophore monensin (20 muM)e2
- Outcome
- IAA-induced stimulation of [(3)H]Ins incorporation and the IAA-induced increase in in vivo extensibilitye3
- Direction
- almost completely inhibitede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“The ionophore monensin (20 muM)”
e3“the IAA-induced stimulation of [(3)H]Ins incorporation and the IAA-induced increase in in vivo extensibility of the sections was almost completely inhibited”
Claim · model-written summary
Monensin did not affect oxygen uptake of pea sections.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- ionophore monensin (20 muM)e2
- Outcome
- oxygen uptakee3
- Direction
- unaffectede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“The ionophore monensin (20 muM)”
e3“although oxygen uptake was unaffected”
Claim · model-written summary
The authors suggest that wall synthesis (as represented by [(3)H]Ins incorporation) and wall loosening (increase in in vivo extensibility) are related processes.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- wall synthesis (as represented by [(3)H]Ins incorporation) and wall loosening (increase in in vivo extensibility) are related processese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“We suggest that wall synthesis (as represented by [(3)H]Ins incorporation) and wall loosening (increase in in vivo extensibility) are related processes”
Claim · model-written summary
The results are interpreted as supporting the hypothesis that IAA induces growth by rapid stimulation of cell wall synthesis in the growth-limiting epidermal cell layer.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- IAAe2
- Outcome
- growthe2
- Qualifier
- rapid stimulation of cell wall synthesis in the growth-limiting epidermal cell layere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pea seedlings”
e2“The results support the hypothesis that IAA induces growth by rapid stimulation of cell wall synthesis in the growth-limiting epidermal cell layer”
The same questions in other papers
monensin A · molecular: metabolites · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.