Abstract
Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth. Kinetin was not essential but was required for optimum growth. Both of the regulators were required for optimum production of extracellular polysaccharide (EP).The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide) isolated from the EP at various stages of the culture cycle.The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments. Changes in composition of the neutral polysaccharide were slight. As a consequence of these results it is now possible to obtain cells differing in the nature of the systems synthesizing, or controlling the synthesis of, the polysaccharides.
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Claims 7
Claim · model-written summary
2,4-dichlorophenoxyacetic acid (2,4-D) was required for growth of bush bean cell suspension cultures.
Not reportedComparatorDirection
Verbatim
e1“Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth”
Claim · model-written summary
Kinetin was not essential but was required for optimum growth of bush bean cell suspension cultures.
Not reportedComparatorDirection
Verbatim
e2“Kinetin was not essential but was required for optimum growth”
e1“Cotyledon cell suspension cultures of bush bean”
Claim · model-written summary
Both regulators were required for optimum production of extracellular polysaccharide.
- Taxon
- bush beane1
- Treatment
- Both of the regulatorse3
- Outcome
- production of extracellular polysaccharidee3
Not reportedComparatorDirection
Verbatim
e3“Both of the regulators were required for optimum production of extracellular polysaccharide (EP)”
e1“Cotyledon cell suspension cultures of bush bean”
Claim · model-written summary
The two regulators had different effects on production of three polysaccharide fractions (two pectins and a neutral polysaccharide).
- Taxon
- bush beane1
- Treatment
- The two regulatorse4
- Outcome
- production of three polysaccharide fractionse4
- Qualifier
- two pectins and a neutral polysaccharidee4
Not reportedComparatorDirection
Verbatim
e4“The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide)”
e1“Cotyledon cell suspension cultures of bush bean”
Claim · model-written summary
Neutral sugar composition of the pectin fractions fluctuated considerably during the culture cycle.
Verbatim
e5“The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments”
e1“Cotyledon cell suspension cultures of bush bean”
Claim · model-written summary
Regulator treatments could change the neutral sugar composition of the pectin fractions markedly.
- Taxon
- bush beane1
- Treatment
- regulator treatmentse5
- Outcome
- neutral sugar composition of the pectin fractionse5
- Direction
- changed markedlye5
Not reportedComparator
Verbatim
e5“The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments”
e1“Cotyledon cell suspension cultures of bush bean”
Claim · model-written summary
Changes in composition of the neutral polysaccharide were slight.
Verbatim
e6“Changes in composition of the neutral polysaccharide were slight”
e1“Cotyledon cell suspension cultures of bush bean”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.