Abstract
The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined. Cells at the logarithmic phase of growth were treated with or without Al in nutrient medium prepared without P i and EDTA. In the absence of Al, total GSH content (including oxidized glutathione [GSSG]) increased gradually. In the presence of Al, the increase of GSH was repressed. This effect was observed before the loss of plasma membrane integrity and the loss of cell viability. In contrast, GSSG content in cells increased in the presence of Al. GSH‐deprived cells were prepared by culturing cells with buthionine sulfoximine (an inhibitor of Γ ‐glutamylcysteine synthetase) for 24 h. Total GSH content in GSH‐deprived cells was 6% of that in normal cells. The GSH‐deprived cells exhibited a higher degree of lipid peroxidation, increased accumulation of Al, and greater loss of viability than normal cells. These results suggest that GSH protects cells from the oxidative membrane damage caused by a combination of Al and Fe(II) possibly by both direct consumption of GSH and oxidation of GSH.
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Claims 8
Claim · model-written summary
Total glutathione content of tobacco cells increased gradually in the absence of Al.
- Taxon
- tobacco cells
Nicotiana tabacume0 - Outcome
- total GSH contente1
- Direction
- increasede1
- Qualifier
- In the absence of Ale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e1“In the absence of Al, total GSH content (including oxidized glutathione [GSSG]) increased gradually”
Claim · model-written summary
Al repressed the increase of GSH in tobacco cells, before loss of membrane integrity and viability.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- Ale2
- Outcome
- increase of GSHe2
- Direction
- repressede2
- Qualifier
- This effect was observed before the loss of plasma membrane integrity and the loss of cell viabilitye3
- Comparator
- In the absence of Ale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e1“In the absence of Al, total GSH content (including oxidized glutathione [GSSG]) increased gradually”
e2“In the presence of Al, the increase of GSH was repressed”
e3“This effect was observed before the loss of plasma membrane integrity and the loss of cell viability”
Claim · model-written summary
Al increased the oxidized glutathione (GSSG) content of tobacco cells.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- Ale4
- Outcome
- GSSG contente4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e4“In contrast, GSSG content in cells increased in the presence of Al”
Claim · model-written summary
Glutathione-deprived tobacco cells have total GSH at 6% of the level in normal cells.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- buthionine sulfoximinee6
- Outcome
- Total GSH contente5
- Direction
- lowerinferrede5
- Value
- 6%e5
- Comparator
- normal cellse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e5“Total GSH content in GSH‐deprived cells was 6% of that in normal cells”
e6“culturing cells with buthionine sulfoximine”
Claim · model-written summary
Glutathione-deprived tobacco cells show a higher degree of lipid peroxidation than normal cells.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- buthionine sulfoximinee6
- Outcome
- lipid peroxidatione7
- Direction
- highere7
- Comparator
- normal cellse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e6“culturing cells with buthionine sulfoximine”
e7“The GSH‐deprived cells exhibited a higher degree of lipid peroxidation, increased accumulation of Al, and greater loss of viability than normal cells”
Claim · model-written summary
Glutathione-deprived tobacco cells accumulate more Al than normal cells.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- buthionine sulfoximinee6
- Outcome
- accumulation of Ale7
- Direction
- increasede7
- Comparator
- normal cellse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e6“culturing cells with buthionine sulfoximine”
e7“The GSH‐deprived cells exhibited a higher degree of lipid peroxidation, increased accumulation of Al, and greater loss of viability than normal cells”
Claim · model-written summary
Glutathione-deprived tobacco cells show a greater loss of viability than normal cells.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- buthionine sulfoximinee6
- Outcome
- loss of viabilitye7
- Direction
- greatere7
- Comparator
- normal cellse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e6“culturing cells with buthionine sulfoximine”
e7“The GSH‐deprived cells exhibited a higher degree of lipid peroxidation, increased accumulation of Al, and greater loss of viability than normal cells”
Claim · model-written summary
Glutathione protects tobacco cells from oxidative membrane damage caused by a combination of Al and Fe(II), possibly through direct consumption and oxidation of GSH.
- Taxon
- tobacco cellsNicotiana tabacume0
- Treatment
- combination of Al and Fe(II)e1
- Compound
- GSHe1
- Outcome
- oxidative membrane damagee1
- Qualifier
- possibly by both direct consumption of GSH and oxidation of GSHe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of endogenous glutathione (GSH) in the protection of suspension‐cultured tobacco cells from aluminum (Al) toxicity was examined”
e1“These results suggest that GSH protects cells from the oxidative membrane damage caused by a combination of Al and Fe(II) possibly by both direct consumption of GSH and oxidation of GSH”
The same questions in other papers
aluminium atom · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
6 papers more in this group
See all 10 papers →S-butyl-DL-homocysteine (S,R)-sulfoximine · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
S-butyl-DL-homocysteine (S,R)-sulfoximine · growth · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.