Abstract
We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The deduced amino acid sequence consists of a polypeptide with 255 amino acids, including a putative transit peptide (40 a.a.) in amino-terminal residues. This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs. The metal analysis and SOD assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD. However this SOD activity was not inhibited by the treatment with hydrogen peroxide, which was expected to inhibit known Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction. These results suggested that this cDNA encodes rice Fe-SOD, which is apparently distinct from known plant Fe-SODs.
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Claims 8
Claim · model-written summary
A rice cDNA encoding Fe-SOD was isolated.
- Taxon
- rice (Oryza sativa L.)
Oryza sativae0 - Gene
- Fe-SODe0
- Outcome
- cDNA encoding Fe-SODe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.).”
Claim · model-written summary
The deduced polypeptide has 255 amino acids.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Outcome
- polypeptidee0
- Value
- 255 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequence consists of a polypeptide with 255 amino acids”
t0“rice (Oryza sativaL.)”
Claim · model-written summary
The sequence includes a putative 40-amino-acid transit peptide.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Outcome
- putative transit peptidee0
- Value
- 40 a.a.e0
- Qualifier
- amino-terminal residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including a putative transit peptide (40 a.a.) in amino-terminal residues”
t0“rice (Oryza sativaL.)”
Claim · model-written summary
The rice sequence resembles known plant Fe-SODs but is not classified with them.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Outcome
- similar to the known plant Fe-SODs but not classified in the group of known Fe-SODse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs.”
t0“rice (Oryza sativaL.)”
Claim · model-written summary
Metal analysis and activity assays identified the recombinant protein as an iron-containing SOD.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Gene
- SODe0
- Outcome
- iron-containing SODe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The metal analysis and SOD assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD.”
t0“rice (Oryza sativaL.)”
Claim · model-written summary
Hydrogen peroxide did not inhibit this SOD activity.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- hydrogen peroxidee0
- Outcome
- SOD activitye0
- Direction
- not inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this SOD activity was not inhibited by the treatment with hydrogen peroxide”
t0“rice (Oryza sativaL.)”
Claim · model-written summary
Rice Fe-SOD mRNA was detected throughout the vegetative tissues examined and was especially abundant in calli.
- Taxon
- riceOryza sativae0
- Organ / stage
- callie0
- Gene
- rice Fe-SODe0
- Outcome
- mRNA of rice Fe-SODe0
- Direction
- especially abundante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli”
Claim · model-written summary
Light strongly increased rice Fe-SOD mRNA.
- Taxon
- riceOryza sativae0
- Treatment
- light inductione0
- Gene
- rice Fe-SODe0
- Outcome
- mRNA of rice Fe-SODe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction”
The same questions in other papers
hydrogen peroxide · physiology: antioxidant enzymes · Oryza sativa (rice)Results differ
3 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →light · molecular: expression · Oryza sativa (rice)Positive and negative results
19 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- negative
- no effect
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.