Abstract
The oxidative stress response of rice cytosolic ascorbate peroxidase (APX) was examined. The transcript level of cytosolic APX was significantly increased when suspension cultures of germinating rice embryos were treated with paraquat (7.9-fold) or H2O2 (6.1-fold). Induction by paraquat reached a maximum at 8 h. Induction by H2O2 peaked earlier at 4 h of treatment. This result suggests that the induction by paraquat might be caused by the H2O2 generated from superoxide. Treatment with a superoxide dismutase inhibitor, diethyldithiocarbamate, which is supposed to decrease the cellular H2O2 level, reduced the paraquat induction of cytosolic APX. In contrast, when APX and catalase were inhibited by hydroxyurea or aminotriazole, cellular H2O2 content was elevated and cytosolic APX mRNA was markedly increased without paraquat or H2O2 treatment. This suggests that cytosolic APX is regulated by the H level within cells. An increase in H2O2 content was observed in the paraquat treated embryos, suggesting that paraquat induction of cytosolic APX was caused by H2O2 generated through superoxide dismutation. These results indicate that H2O2 is involved in oxidative stress signalling, leading to the induction of cytosolic APX.
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Claims 11
Claim · model-written summary
Paraquat increased cytosolic APX transcript 7.9 fold.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- germinating rice embryose0
- Treatment
- paraquate0
- Gene
- cytosolic APXe0
- Outcome
- transcript level of cytosolic APXe0
- Direction
- increasede0
- Value
- 7.9-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of cytosolic APX was significantly increased when suspension cultures of germinating rice embryos were treated with paraquat (7.9-fold) or H2O2 (6.1-fold)”
Claim · model-written summary
H2O2 increased cytosolic APX transcript 6.1 fold.
- Taxon
- riceOryza sativae0
- Organ / stage
- germinating rice embryose0
- Treatment
- H2O2e0
- Gene
- cytosolic APXe0
- Outcome
- transcript level of cytosolic APXe0
- Direction
- increasede0
- Value
- 6.1-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of cytosolic APX was significantly increased when suspension cultures of germinating rice embryos were treated with paraquat (7.9-fold) or H2O2 (6.1-fold)”
Claim · model-written summary
Paraquat induction peaked at eight hours.
- Taxon
- RiceOryza sativat0
- Treatment
- paraquate0
- Outcome
- Induction by paraquate0
- Direction
- maximume0
- Value
- 8 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induction by paraquat reached a maximum at 8 h”
t0“Rice”
Claim · model-written summary
H2O2 induction peaked at four hours.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Induction by H2O2 peaked earlier at 4 h of treatment”
t0“Rice”
Claim · model-written summary
Diethyldithiocarbamate reduced paraquat induction of cytosolic APX.
- Taxon
- RiceOryza sativat0
- Treatment
- diethyldithiocarbamatee0
- Gene
- cytosolic APXe0
- Outcome
- paraquat induction of cytosolic APXe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with a superoxide dismutase inhibitor, diethyldithiocarbamate, which is supposed to decrease the cellular H2O2 level, reduced the paraquat induction of cytosolic APX”
t0“Rice”
Claim · model-written summary
Hydroxyurea inhibition of APX and catalase elevated cellular H2O2.
- Taxon
- RiceOryza sativat0
- Treatment
- hydroxyureae0
- Outcome
- cellular H2O2 contente0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when APX and catalase were inhibited by hydroxyurea or aminotriazole, cellular H2O2 content was elevated and cytosolic APX mRNA was markedly increased without paraquat or H2O2 treatment”
t0“Rice”
Claim · model-written summary
Aminotriazole inhibition of APX and catalase elevated cellular H2O2.
- Taxon
- RiceOryza sativat0
- Treatment
- aminotriazolee0
- Outcome
- cellular H2O2 contente0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when APX and catalase were inhibited by hydroxyurea or aminotriazole, cellular H2O2 content was elevated and cytosolic APX mRNA was markedly increased without paraquat or H2O2 treatment”
t0“Rice”
Claim · model-written summary
Hydroxyurea inhibition of APX and catalase increased cytosolic APX mRNA.
- Taxon
- RiceOryza sativat0
- Treatment
- hydroxyureae0
- Outcome
- cytosolic APX mRNAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when APX and catalase were inhibited by hydroxyurea or aminotriazole, cellular H2O2 content was elevated and cytosolic APX mRNA was markedly increased without paraquat or H2O2 treatment”
t0“Rice”
Claim · model-written summary
Aminotriazole inhibition of APX and catalase increased cytosolic APX mRNA.
- Taxon
- RiceOryza sativat0
- Treatment
- aminotriazolee0
- Outcome
- cytosolic APX mRNAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when APX and catalase were inhibited by hydroxyurea or aminotriazole, cellular H2O2 content was elevated and cytosolic APX mRNA was markedly increased without paraquat or H2O2 treatment”
t0“Rice”
Claim · model-written summary
Paraquat increased H2O2 content in embryos.
- Taxon
- RiceOryza sativat0
- Treatment
- paraquate0
- Outcome
- H2O2 contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increase in H2O2 content was observed in the paraquat treated embryos”
t0“Rice”
Claim · model-written summary
H2O2 generated by superoxide dismutation was proposed to mediate paraquat induction of APX.
- Taxon
- RiceOryza sativat0
- Compound
- H2O2e0
- Outcome
- paraquat induction of cytosolic APXe0
- Qualifier
- superoxide dismutatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“paraquat induction of cytosolic APX was caused by H2O2 generated through superoxide dismutation”
t0“Rice”
The same questions in other papers
hydrogen peroxide · physiology: antioxidant enzymes · Oryza sativa (rice)Results differ
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
6 papers more in this group
See all 10 papers →paraquat · physiology: antioxidant enzymes · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.