Abstract
Glutaredoxins (GRXs) catalyze the reduction of protein disulfide bonds using glutathione as a reductant. Certain GRXs are able to transfer iron-sulfur clusters to other proteins. To investigate the function of Arabidopsis (Arabidopsis thaliana) GRXS17, we applied a strategy combining biochemical, genetic, and physiological approaches. GRXS17 was localized in the nucleus and cytosol, and its expression was elevated in the shoot meristems and reproductive tissues. Recombinant GRXS17 bound Fe2S2 clusters, a property likely contributing to its ability to complement the defects of a Baker's yeast (Saccharomyces cerevisiae) strain lacking the mitochondrial GRX5. However, a grxs17 knockout Arabidopsis mutant exhibited only a minor decrease in the activities of iron-sulfur enzymes, suggesting that its primary function is as a disulfide oxidoreductase. The grxS17 plants were sensitive to high temperatures and long-day photoperiods, resulting in elongated leaves, compromised shoot apical meristem, and delayed bolting. Both environmental conditions applied simultaneously led to a growth arrest. Using affinity chromatography and split-Yellow Fluorescent Protein methods, a nuclear transcriptional regulator, the Nuclear Factor Y Subunit C11/Negative Cofactor 2α (NF-YC11/NC2α), was identified as a GRXS17 interacting partner. A mutant deficient in NF-YC11/NC2α exhibited similar phenotypes to grxs17 in response to photoperiod. Therefore, we propose that GRXS17 interacts with NF-YC11/NC2α to relay a redox signal generated by the photoperiod to maintain meristem function.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
GRXS17 was found in the nucleus and cytosol.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- GRXS17e0
- Outcome
- localized in the nucleus and cytosole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GRXS17 was localized in the nucleus and cytosol”
t0“Arabidopsis”
Claim · model-written summary
GRXS17 expression was elevated in shoot meristems and reproductive tissues.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- shoot meristems and reproductive tissuese0
- Gene
- GRXS17e1
- Outcome
- expressione0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression was elevated in the shoot meristems and reproductive tissues”
t0“Arabidopsis”
e1“Arabidopsis (Arabidopsis thaliana) GRXS17”
Claim · model-written summary
Recombinant GRXS17 bound Fe2S2 clusters.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GRXS17e0
- Outcome
- bound Fe2S2 clusterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recombinant GRXS17 bound Fe2S2 clusters”
t0“Arabidopsis”
Claim · model-written summary
GRXS17 complemented defects of a yeast GRX5 deficient strain.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- GRXS17e1
- Interactor
- Saccharomyces cerevisiae
Saccharomyces cerevisiaee0other - Gene
- GRXS17e1
- Outcome
- complement the defectse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its ability to complement the defects of a Baker's yeast (Saccharomyces cerevisiae) strain lacking the mitochondrial GRX5”
t0“Arabidopsis”
e1“Arabidopsis (Arabidopsis thaliana) GRXS17”
Claim · model-written summary
The grxs17 knockout had a minor decrease in iron-sulfur enzyme activities.
- Taxon
- ArabidopsisArabidopsise0
- Material
- grxs17 knockout Arabidopsis mutante0
- Outcome
- activities of iron-sulfur enzymese0
- Direction
- decreasee0
- Qualifier
- only a minore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a grxs17 knockout Arabidopsis mutant exhibited only a minor decrease in the activities of iron-sulfur enzymes”
Claim · model-written summary
grxS17 plants were sensitive to high temperatures and long-day photoperiods, with elongated leaves, compromised shoot apical meristem and delayed bolting.
- Taxon
- ArabidopsisArabidopsist0
- Material
- grxS17 plantse0
- Treatment
- high temperatures and long-day photoperiodse0
- Outcome
- elongated leaves, compromised shoot apical meristem, and delayed boltinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The grxS17 plants were sensitive to high temperatures and long-day photoperiods, resulting in elongated leaves, compromised shoot apical meristem, and delayed bolting”
t0“Arabidopsis”
Claim · model-written summary
High temperature and long-day photoperiod together arrested growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Both environmental conditions applied simultaneouslye0
- Outcome
- growth arreste0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both environmental conditions applied simultaneously led to a growth arrest”
t0“Arabidopsis”
Claim · model-written summary
NF-YC11/NC2α was identified as a GRXS17 partner.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NF-YC11/NC2αe0
- Outcome
- GRXS17 interacting partnere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Nuclear Factor Y Subunit C11/Negative Cofactor 2α (NF-YC11/NC2α), was identified as a GRXS17 interacting partner”
t0“Arabidopsis”
Claim · model-written summary
An NF-YC11/NC2α deficient mutant had photoperiod response phenotypes similar to grxs17.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NF-YC11/NC2αe0
- Outcome
- similar phenotypes to grxs17e0
- Comparator
- grxs17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mutant deficient in NF-YC11/NC2α exhibited similar phenotypes to grxs17 in response to photoperiod”
t0“Arabidopsis”
Claim · model-written summary
The authors propose GRXS17 and NF-YC11/NC2α relay a photoperiod redox signal to maintain meristem function.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GRXS17e0
- Outcome
- maintain meristem functione0
- Qualifier
- relay a redox signal generated by the photoperiode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GRXS17 interacts with NF-YC11/NC2α to relay a redox signal generated by the photoperiod to maintain meristem function”
t0“Arabidopsis”
The same questions in other papers
climate site · growth · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
3 papers more in this group
See all 7 papers →light · phenology · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →heat · phenology · ArabidopsisResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.