Abstract
A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment and can be detected on immunoblots by an antiserum raised against barley lactate dehydrogenase. The activity present in aerobic seedlings was partially purified. The native enzyme has a molecular mass of 160 kilodaltons, and is a tetramer of 2 subunit (38 and 39 kilodaltons) randomly associated. Studies of substrate specificity, native gel electrophoresis, and immunoblot analysis indicate that the partially purified enzyme is a typical lactate dehydrogenase. However, no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia.
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Claims 10
Claim · model-written summary
Lactate dehydrogenase activity was present in rice seedlings and roots.
- Taxon
- Oryza sativa L.
Oryza sativae0 - Organ / stage
- seedlings and rootse0
- Gene
- lactate dehydrogenasee0
- Outcome
- activity is presente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots”
Claim · model-written summary
Under aerobic conditions, lactate dehydrogenase activity was barely detectable in rice seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Gene
- lactate dehydrogenasee0
- Outcome
- activitye0
- Direction
- barely detectablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings”
Claim · model-written summary
Under aerobic conditions, lactate dehydrogenase activity was very low in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Gene
- lactate dehydrogenasee0
- Outcome
- activitye0
- Direction
- very lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots”
Claim · model-written summary
Anoxic or hypoxic treatment increased activity two to three times in 30 day old roots.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- 30 day old rootse0
- Treatment
- anoxic or hypoxic treatmente0
- Outcome
- activitye0
- Direction
- increasede0
- Value
- two to three timese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment”
t0“Oryza sativa L.”
Claim · model-written summary
Lactate dehydrogenase in treated roots was detected on immunoblots.
- Taxon
- Oryza sativa L.Oryza sativat0
- Gene
- lactate dehydrogenasee0
- Outcome
- detected on immunoblotse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can be detected on immunoblots by an antiserum raised against barley lactate dehydrogenase”
t0“Oryza sativa L.”
Claim · model-written summary
The purified native enzyme had a molecular mass of 160 kilodaltons.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- molecular masse0
- Value
- 160 kilodaltonse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The native enzyme has a molecular mass of 160 kilodaltons”
t0“Oryza sativa L.”
Claim · model-written summary
The enzyme was a tetramer with 38 and 39 kilodalton subunits randomly associated.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- tetramer of 2 subunit (38 and 39 kilodaltons) randomly associatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is a tetramer of 2 subunit (38 and 39 kilodaltons) randomly associated”
t0“Oryza sativa L.”
Claim · model-written summary
Substrate specificity, electrophoresis and immunoblots characterized the enzyme as typical lactate dehydrogenase.
- Taxon
- Oryza sativa L.Oryza sativat0
- Outcome
- typical lactate dehydrogenasee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Studies of substrate specificity, native gel electrophoresis, and immunoblot analysis indicate that the partially purified enzyme is a typical lactate dehydrogenase”
t0“Oryza sativa L.”
Claim · model-written summary
Seedlings transferred to anoxia did not increase lactate dehydrogenase activity.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- transferred to anoxiae0
- Outcome
- lactate dehydrogenase activitye0
- Direction
- no increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia”
t0“Oryza sativa L.”
Claim · model-written summary
Seedlings transferred to anoxia did not increase lactate dehydrogenase protein.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- transferred to anoxiae0
- Outcome
- proteine0
- Direction
- no increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia”
t0“Oryza sativa L.”
The same questions in other papers
flooding · soil: biology · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
flooding · quality: protein · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.