Abstract
Flash flooding (FF) along with bacterial blight (BB) outbreak are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem. The presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL. In this context, the marker-assisted improvement of plant resistance/tolerance has been proven as one of the most promising strategies towards the development of sustainable cultivars. The present study demonstrates the marker-assisted introgression of the submergence tolerant (Sub1) and three bacterial blight resistant genes (Xa21 + xa13 + xa5) into the genetic background of Hasanta, a long duration popular rice variety in the eastern coastal region of India. The rice genotypes, Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21 + xa13 + Xa7 + xa5 + Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta; recurrent parent (RP) was used as donor. The forward analysis of target genes in F1s, IC1F1s and backcross (BC) generations was performed by linked/genic markers (Sub1bc2; pTA248, xa13prom and RM122), whereas background recovery of RP in each BC and segregating generations was performed utilizing 108 hypervariable SSR markers. Intervened speed breeding (SB) strategy and intensive phenotyping could lead the development of near isogenic lines (NILs) as to the RP in all basic traits. The performance of the near isogenic lines (NILs, BC2F3 and BC2F4), HS 232-411-391-756-37, HS 232-411-391-809-8, HS 232-411-391-756-18, HS 110-224-197-10-36, HS 232-411-391-809-81, HS 110-224-197-10-41 and HS 232-411-391-809-63 establishes the utility of marker-assisted backcross-breeding (MAB) and SB in accelerated trait introgression. The introgressed lines carrying Sub1 + Xa21 + xa13 + xa5 showed 76% to 91% survival under 14 days of submergence and durable BB resistance (percent disease index-PDI of 2.68 ± 0.26 to 6.22 ± 1.08 and lesion length, LL of 1.29 ± 0.12 to 4.2 ± 0.64 cm). Physiological analysis revealed that improved NILs, carrying Sub1 gene conquered adaptive physiological modulations, had reduced the consumption of soluble sugar and the degradation of total chlorophyll contents (TCC), and an enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes. The pyramided lines attained complete product profile of RP, that will contribute to sustainable rice production under RSL, particularly in the coastal region that has substantial acreage under the variety Hasanta.
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Claims 11
Claim · model-written summary
Swarna Sub1 had genetic similarity of 0.84 to Hasanta.
- Taxon
- rice
Oryza sativat0 - Material
- Swarna Sub1e0
- Gene
- Sub1e0
- Outcome
- genetic similaritye0
- Direction
- maximume0
- Value
- 0.84e0
- Comparator
- Hasantae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21 + xa13 + Xa7 + xa5 + Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta”
t0“Rice Variety Hasanta”
Claim · model-written summary
IRBB66 had genetic similarity of 0.73 to Hasanta.
- Taxon
- riceOryza sativat0
- Material
- IRBB66e0
- Outcome
- genetic similaritye0
- Direction
- maximume0
- Value
- 0.73e0
- Comparator
- Hasantae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21 + xa13 + Xa7 + xa5 + Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta”
t0“Rice Variety Hasanta”
Claim · model-written summary
Near isogenic lines were developed with basic traits resembling Hasanta.
- Taxon
- riceOryza sativat0
- Outcome
- near isogenic lines (NILs)e0
- Qualifier
- in all basic traitse0
- Comparator
- RPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Intervened speed breeding (SB) strategy and intensive phenotyping could lead the development of near isogenic lines (NILs) as to the RP in all basic traits”
t0“Rice Variety Hasanta”
Claim · model-written summary
Introgressed lines survived 14 days of submergence at 76% to 91%.
- Taxon
- riceOryza sativat0
- Material
- introgressed linese0
- Treatment
- 14 days of submergencee0
- Gene
- Sub1 + Xa21 + xa13 + xa5e0
- Outcome
- survivale0
- Value
- 76% to 91%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The introgressed lines carrying Sub1 + Xa21 + xa13 + xa5 showed 76% to 91% survival under 14 days of submergence”
t0“Rice Variety Hasanta”
Claim · model-written summary
Introgressed lines had bacterial blight disease indices from 2.68 ± 0.26 to 6.22 ± 1.08.
- Taxon
- riceOryza sativat0
- Outcome
- percent disease index-PDIe0
- Value
- 2.68 ± 0.26 to 6.22 ± 1.08e0
- Qualifier
- durable BB resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“durable BB resistance (percent disease index-PDI of 2.68 ± 0.26 to 6.22 ± 1.08 and lesion length, LL of 1.29 ± 0.12 to 4.2 ± 0.64 cm)”
t0“Rice Variety Hasanta”
Claim · model-written summary
Introgressed lines had bacterial blight lesion lengths from 1.29 ± 0.12 to 4.2 ± 0.64 cm.
- Taxon
- riceOryza sativat0
- Outcome
- lesion lengthe0
- Value
- 1.29 ± 0.12 to 4.2 ± 0.64 cme0
- Qualifier
- durable BB resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“durable BB resistance (percent disease index-PDI of 2.68 ± 0.26 to 6.22 ± 1.08 and lesion length, LL of 1.29 ± 0.12 to 4.2 ± 0.64 cm)”
t0“Rice Variety Hasanta”
Claim · model-written summary
Sub1 near isogenic lines consumed less soluble sugar under submergence.
- Taxon
- riceOryza sativat0
- Material
- improved NILse0
- Treatment
- submergence regimest1
- Gene
- Sub1e0
- Outcome
- consumption of soluble sugare0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improved NILs, carrying Sub1 gene conquered adaptive physiological modulations, had reduced the consumption of soluble sugar and the degradation of total chlorophyll contents (TCC)”
t0“Rice Variety Hasanta”
t1“submergence regimes”
Claim · model-written summary
Sub1 near isogenic lines had reduced degradation of total chlorophyll.
- Taxon
- riceOryza sativat0
- Material
- improved NILse0
- Gene
- Sub1e0
- Outcome
- degradation of total chlorophyll contents (TCC)e0
- Direction
- reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improved NILs, carrying Sub1 gene conquered adaptive physiological modulations, had reduced the consumption of soluble sugar and the degradation of total chlorophyll contents (TCC)”
t0“Rice Variety Hasanta”
Claim · model-written summary
Sub1 near isogenic lines had enhanced alcohol dehydrogenase activity during submergence.
- Taxon
- riceOryza sativat0
- Treatment
- submergence regimese0
- Outcome
- Alcohol Dehydrogenase activity (ADH)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes”
t0“Rice Variety Hasanta”
Claim · model-written summary
Sub1 near isogenic lines had enhanced proline accumulation during submergence.
- Taxon
- riceOryza sativat0
- Treatment
- submergence regimese0
- Outcome
- proline accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes”
t0“Rice Variety Hasanta”
Claim · model-written summary
Pyramided lines attained the recurrent parent product profile.
- Taxon
- riceOryza sativat0
- Material
- pyramided linese0
- Outcome
- complete product profilee0
- Comparator
- RPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pyramided lines attained complete product profile of RP”
t0“Rice Variety Hasanta”
The same questions in other papers
flooding · physiology: osmolytes · Oryza sativa (rice)Results differ
7 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
8 papers more in this group
See all 12 papers →flooding · soil: biology · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.