Abstract
Flash flooding (FF) alongwith bacterial blight (BB) outbreak are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem. Presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL. In this context, marker-assisted improvement of plant resistance/tolerance has been proven as one of the most promising strategies towards development of sustainable cultivars. The present study demonstrates the marker-assisted introgression of the submergence tolerant (Sub1) and three bacterial blight resistant genes (xa5+xa13+Xa21) into the genetic background of Hasanta, a long duration popular rice variety in eastern coastal region of India. The rice genotypes, Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21+xa13+Xa7+xa5+Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta, recurrent parent (RP) were used as donor. Forward analysis of target genes in F1s, IC1F1s and backcross generations was performed by linked/genic markers (Sub1bc2; pTA248, xa13-prom and RM122), whereas, background recovery of RP in each backcross (BC) and segregating generations was performed utilizing 108 hypervariable SSR markers. Intervened speed breeding (SB) strategy and intensive phenotyping could led development of near isogenic lines (NILs) as to the RP in all basic traits. The performance of the near isogenic lines (NILs, BC2F3 and BC2F4), HS232-411-391-756-37, HS232-411-391-809-8, HS232-411-391-756-18, HS110-224-197-10-36, HS232-411-391-809-81, HS110-224-197-10-41 and HS232-411-391-809-63 establishes the utility of marker-assisted backcross-breeding (MAB) and SB in accelerated trait introgression. The introgressed lines carrying Sub1+Xa21+xa13+xa5 showed 76% to 91% survival under 14 days of submergence and durable BB resistance (percent disease index-PDI of 2.68± 0.26 to 6.22 ± 1.08 and lesion length-LL of 1.29±0.12 cm to 4.2±0.64 cm). Physiological analysis revealed that improved NILs carrying Sub1 gene conquered adaptive physiological modulations, had reduced consumption of soluble sugar and degradation of total chlorophyll contents (TCC); and enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes. The pyramided lines attained complete product profile of RP, that will contribute to sustainable rice production under RSL, particularly in the coastal region that has substantial acreage under variety Hasanta.
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Claims 12
Claim · model-written summary
Introgressed lines carrying Sub1+Xa21+xa13+xa5 showed 76% to 91% survival under 14 days of submergence.
- Taxon
- rice
Oryza sativae0 - Material
- introgressed lines carrying Sub1+Xa21+xa13+xa5e1
- Treatment
- 14 days of submergencee1
- Outcome
- survivale1
- Value
- 76% to 91%e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e1“The introgressed lines carrying Sub1+Xa21+xa13+xa5 showed 76% to 91% survival under 14 days of submergence”
Claim · model-written summary
Introgressed lines carrying Sub1+Xa21+xa13+xa5 had a percent disease index of 2.68 to 6.22 against Xoo.
- Taxon
- riceOryza sativae0
- Material
- introgressed lines carrying Sub1+Xa21+xa13+xa5e1
- Interactor
- Xooe3pathogen
- Outcome
- percent disease index-PDIe2
- Value
- 2.68± 0.26 to 6.22 ± 1.08e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e1“The introgressed lines carrying Sub1+Xa21+xa13+xa5 showed 76% to 91% survival under 14 days of submergence”
e2“durable BB resistance (percent disease index-PDI of 2.68± 0.26 to 6.22 ± 1.08 and lesion length-LL of 1.29±0.12 cm to 4.2±0.64 cm)”
e3“Presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL”
Claim · model-written summary
Introgressed lines carrying Sub1+Xa21+xa13+xa5 had lesion lengths of 1.29 to 4.2 cm against Xoo.
- Taxon
- riceOryza sativae0
- Material
- introgressed lines carrying Sub1+Xa21+xa13+xa5e1
- Interactor
- Xooe3pathogen
- Outcome
- lesion length-LLe2
- Value
- 1.29±0.12 cm to 4.2±0.64 cme2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e1“The introgressed lines carrying Sub1+Xa21+xa13+xa5 showed 76% to 91% survival under 14 days of submergence”
e2“durable BB resistance (percent disease index-PDI of 2.68± 0.26 to 6.22 ± 1.08 and lesion length-LL of 1.29±0.12 cm to 4.2±0.64 cm)”
e3“Presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL”
Claim · model-written summary
Improved NILs carrying Sub1 had reduced consumption of soluble sugar under all submergence regimes.
- Taxon
- riceOryza sativae0
- Material
- improved NILs carrying Sub1 genee4
- Treatment
- all submergence regimese4
- Outcome
- consumption of soluble sugare4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e4“improved NILs carrying Sub1 gene conquered adaptive physiological modulations, had reduced consumption of soluble sugar and degradation of total chlorophyll contents (TCC); and enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes”
Claim · model-written summary
Improved NILs carrying Sub1 had reduced degradation of total chlorophyll under all submergence regimes.
- Taxon
- riceOryza sativae0
- Material
- improved NILs carrying Sub1 genee4
- Treatment
- all submergence regimese4
- Outcome
- degradation of total chlorophyll contents (TCC)e4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e4“improved NILs carrying Sub1 gene conquered adaptive physiological modulations, had reduced consumption of soluble sugar and degradation of total chlorophyll contents (TCC); and enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes”
Claim · model-written summary
Improved NILs carrying Sub1 had enhanced alcohol dehydrogenase activity under all submergence regimes.
- Taxon
- riceOryza sativae0
- Material
- improved NILs carrying Sub1 genee4
- Treatment
- all submergence regimese4
- Outcome
- Alcohol Dehydrogenase activity (ADH)e4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e4“improved NILs carrying Sub1 gene conquered adaptive physiological modulations, had reduced consumption of soluble sugar and degradation of total chlorophyll contents (TCC); and enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes”
Claim · model-written summary
Improved NILs carrying Sub1 had enhanced proline accumulation under all submergence regimes.
- Taxon
- riceOryza sativae0
- Material
- improved NILs carrying Sub1 genee4
- Treatment
- all submergence regimese4
- Outcome
- proline accumulatione4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e4“improved NILs carrying Sub1 gene conquered adaptive physiological modulations, had reduced consumption of soluble sugar and degradation of total chlorophyll contents (TCC); and enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes”
Claim · model-written summary
Speed breeding and intensive phenotyping developed near isogenic lines of Hasanta (RP) in all basic traits.
- Taxon
- riceOryza sativae0
- Outcome
- development of near isogenic lines (NILs)e5
- Comparator
- RPe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e5“Intervened speed breeding (SB) strategy and intensive phenotyping could led development of near isogenic lines (NILs) as to the RP in all basic traits”
Claim · model-written summary
The pyramided lines attained the complete product profile of the recurrent parent.
- Taxon
- riceOryza sativae0
- Material
- pyramided linese6
- Outcome
- complete product profile of RPe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e6“The pyramided lines attained complete product profile of RP”
Claim · model-written summary
Marker-assisted backcross breeding and speed breeding establish utility for accelerated trait introgression.
- Taxon
- riceOryza sativae0
- Outcome
- accelerated trait introgressione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e7“establishes the utility of marker-assisted backcross-breeding (MAB) and SB in accelerated trait introgression”
Claim · model-written summary
Swarna Sub1 had a genetic similarity of 0.84 with Hasanta.
- Taxon
- riceOryza sativae0
- Material
- Swarna Sub1e1
- Outcome
- genetic similaritye1
- Value
- 0.84e1
- Comparator
- Hasantae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e1“The rice genotypes, Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21+xa13+Xa7+xa5+Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta, recurrent parent (RP) were used as donor”
Claim · model-written summary
IRBB66 had a genetic similarity of 0.73 with Hasanta.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem”
e1“The rice genotypes, Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21+xa13+Xa7+xa5+Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta, recurrent parent (RP) were used as donor”
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