Abstract
Abstract: Regulation of uptake and compartmentation of metal ions is important for the maintenance of metal ion homeostasis. To identify mechanisms involved in the protection of plants from Mn toxicity, wild‐type yeast was transformed with an Arabidopsis cDNA library and transformants were screened on toxic Mn concentrations. Wild‐type yeast could not grow in the presence of 30 mM MnSO 4 , while two transformants carrying variants of the same gene were able to grow. Database searches revealed that the isolated cDNAs correspond to AtCAX2, previously described as a vacuolar calcium‐proton antiporter. Since no other genes could be identified, AtCAX2 might represent a major function permitting Mn detoxification in this suppressor screen. Furthermore, yeast transformed with the two AtCAX2 cDNAs showed increased sensitivity towards hydrogen peroxide, pointing to a limited availability of cytoplasmic Mn in the presence of AtCAX2 activity. The open reading frames of the cDNA encoded polypeptides that have a 42 and a 92 amino acids shorter N‐terminal region relative to the predicted full‐length coding region of AtCAX2. In contrast to both truncated cDNAs, the full‐length clone was unable to confer Mn resistance to yeast, indicating that, similar to AtCAX1, AtCAX2 also carries an autoinhibitory N‐terminal domain regulating the activity of AtCAX2.
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Claims 6
Claim · model-written summary
Two yeast transformants carrying variants of one Arabidopsis gene grew at toxic manganese concentrations whereas wild type did not.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- transformants carrying variants of the same genee0
- Outcome
- growe0
- Value
- 30 mM MnSO 4e0
- Comparator
- Wild‐type yeaste0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild‐type yeast could not grow in the presence of 30 mM MnSO 4 , while two transformants carrying variants of the same gene were able to grow”
e1“an Arabidopsis cDNA library”
Claim · model-written summary
The selected cDNAs corresponded to AtCAX2.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- AtCAX2e0
- Outcome
- isolated cDNAs correspond to AtCAX2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the isolated cDNAs correspond to AtCAX2”
t0“A Putative Role for the Vacuolar Calcium/Manganese Proton Antiporter AtCAX2 in Heavy Metal Detoxification”
e1“an Arabidopsis cDNA library”
Claim · model-written summary
AtCAX2 transformed yeast were more sensitive to hydrogen peroxide.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transformed with the two AtCAX2 cDNAse0
- Gene
- AtCAX2e0
- Outcome
- sensitivity towards hydrogen peroxidee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“yeast transformed with the two AtCAX2 cDNAs showed increased sensitivity towards hydrogen peroxide”
t0“A Putative Role for the Vacuolar Calcium/Manganese Proton Antiporter AtCAX2 in Heavy Metal Detoxification”
e1“an Arabidopsis cDNA library”
Claim · model-written summary
The cDNA proteins had N termini shorter by 42 and 92 amino acids.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- AtCAX2e0
- Outcome
- N‐terminal regione0
- Value
- 42 and a 92 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The open reading frames of the cDNA encoded polypeptides that have a 42 and a 92 amino acids shorter N‐terminal region relative to the predicted full‐length coding region of AtCAX2”
t0“A Putative Role for the Vacuolar Calcium/Manganese Proton Antiporter AtCAX2 in Heavy Metal Detoxification”
e1“an Arabidopsis cDNA library”
Claim · model-written summary
Full-length AtCAX2 did not confer manganese resistance in yeast unlike the truncated cDNAs.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- full‐length clonee0
- Gene
- AtCAX2t0
- Outcome
- Mn resistance to yeaste0
- Direction
- unable to confere0
- Comparator
- truncated cDNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast to both truncated cDNAs, the full‐length clone was unable to confer Mn resistance to yeast”
t0“A Putative Role for the Vacuolar Calcium/Manganese Proton Antiporter AtCAX2 in Heavy Metal Detoxification”
e1“an Arabidopsis cDNA library”
Claim · model-written summary
The authors infer an autoinhibitory N-terminal domain regulating AtCAX2 activity.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- AtCAX2e0
- Outcome
- activity of AtCAX2e0
- Qualifier
- autoinhibitory N‐terminal domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtCAX2 also carries an autoinhibitory N‐terminal domain regulating the activity of AtCAX2”
t0“A Putative Role for the Vacuolar Calcium/Manganese Proton Antiporter AtCAX2 in Heavy Metal Detoxification”
e1“an Arabidopsis cDNA library”
The same questions in other papers
transgenic · physiology: oxidative · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.