Abstract
The activity of the enzyme ribulose bisphosphate carboxylase (RuBPCase) was estimated after rapidly extracting it from intact wheat leaves pretreated under different light and CO2 levels. No HCO3− was added to the extraction buffer since it is shown to inhibit RuBPCase. The activity increased as light intensity or CO2 concentration during pretreatment was increased. Enzyme activity increased as temperature during pretreatment was decreased. Light activation did not affect the affinity of RuBPCase for CO2. A Km of 30 μM CO2 under air level O2 was determined. CO2, light and temperature are three main limiting factors of photosynthesis. It seems that the activity of RuBPCase is regulated by these factors according to the requirements for CO2 fixation.
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Claims 5
Claim · model-written summary
RuBPCase activity in wheat leaves increased as light intensity or CO2 concentration during pretreatment was increased.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- light intensity or CO2 concentration during pretreatmente1
- Gene
- ribulose bisphosphate carboxylase (RuBPCase)e0
- Outcome
- activitye1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme ribulose bisphosphate carboxylase (RuBPCase) was estimated after rapidly extracting it from intact wheat leaves pretreated under different light and CO2 levels”
e1“The activity increased as light intensity or CO2 concentration during pretreatment was increased”
Claim · model-written summary
RuBPCase activity in wheat leaves increased as temperature during pretreatment was decreased.
- Taxon
- wheatTriticum aestivume0
- Treatment
- temperature during pretreatmente1
- Gene
- ribulose bisphosphate carboxylase (RuBPCase)e0
- Outcome
- Enzyme activitye1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme ribulose bisphosphate carboxylase (RuBPCase) was estimated after rapidly extracting it from intact wheat leaves pretreated under different light and CO2 levels”
e1“Enzyme activity increased as temperature during pretreatment was decreased”
Claim · model-written summary
Light activation did not affect the affinity of RuBPCase for CO2 in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Light activatione1
- Gene
- RuBPCasee1
- Outcome
- affinity of RuBPCase for CO2e1
- Direction
- did not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme ribulose bisphosphate carboxylase (RuBPCase) was estimated after rapidly extracting it from intact wheat leaves pretreated under different light and CO2 levels”
e1“Light activation did not affect the affinity of RuBPCase for CO2”
Claim · model-written summary
The Km of RuBPCase for CO2 under air-level O2 was 30 μM CO2.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme ribulose bisphosphate carboxylase (RuBPCase) was estimated after rapidly extracting it from intact wheat leaves pretreated under different light and CO2 levels”
e1“A Km of 30 μM CO2 under air level O2 was determined”
Claim · model-written summary
RuBPCase activity in wheat may be regulated by CO2, light and temperature according to the requirements for CO2 fixation.
- Taxon
- wheatTriticum aestivume0
- Outcome
- activity of RuBPCasee1
- Qualifier
- according to the requirements for CO2 fixatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme ribulose bisphosphate carboxylase (RuBPCase) was estimated after rapidly extracting it from intact wheat leaves pretreated under different light and CO2 levels”
e1“It seems that the activity of RuBPCase is regulated by these factors according to the requirements for CO2 fixation”
The same questions in other papers
light · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
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