Abstract
The function of a putative glycosyltransferase (At2g35100) was investigated in Arabidopsis (Arabidopsis thaliana). The protein is predicted to be a type 2 membrane protein with a signal anchor. Two independent mutant lines with T-DNA insertion in the ARABINAN DEFICIENT 1 (ARAD1) gene were analyzed. The gene was shown to be expressed in all tissues but particularly in vascular tissues of leaves and stems. Analysis of cell wall polysaccharides isolated from leaves and stems showed that arabinose content was reduced to about 75% and 46%, respectively, of wild-type levels. Immunohistochemical analysis indicated a specific decrease in arabinan with no change in other pectic domains or in glycoproteins. The cellular structure of the stem was also not altered. Isolated rhamnogalacturonan I from mutant tissues contained only about 30% of the wild-type amount of arabinose, confirming the specific deficiency in arabinan. Linkage analysis showed that the small amount of arabinan present in mutant tissue was structurally similar to that of the wild type. Transformation of mutant plants with the ARAD1 gene driven by the 35S promoter led to full complementation of the phenotype, but none of the transformants had more arabinan than the wild-type level. The data suggest that ARAD1 is an arabinan alpha-1,5-arabinosyltransferase. To our knowledge, the identification of other L-arabinosyltransferases has not been published.
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Claims 11
Claim · model-written summary
ARAD1 was expressed in all tissues, particularly vascular tissues of leaves and stems.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- expressed in all tissues but particularly in vascular tissues of leaves and stemse0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene was shown to be expressed in all tissues but particularly in vascular tissues of leaves and stems”
t0“Arabidopsis”
Claim · model-written summary
Mutant leaves had reduced arabinose content, to about 75% of wild-type levels.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- leavese0
- Outcome
- arabinose contente0
- Direction
- reducede0
- Value
- about 75%e0
- Comparator
- wild-type levelse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of cell wall polysaccharides isolated from leaves and stems showed that arabinose content was reduced to about 75% and 46%, respectively, of wild-type levels”
t0“Arabidopsis”
Claim · model-written summary
Mutant stems had reduced arabinose content, to 46% of wild-type levels.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- stemse0
- Outcome
- arabinose contente0
- Direction
- reducede0
- Value
- 46%e0
- Comparator
- wild-type levelse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of cell wall polysaccharides isolated from leaves and stems showed that arabinose content was reduced to about 75% and 46%, respectively, of wild-type levels”
t0“Arabidopsis”
Claim · model-written summary
Mutant tissue had a specific decrease in arabinan.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- arabinane0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunohistochemical analysis indicated a specific decrease in arabinan with no change in other pectic domains or in glycoproteins”
t0“Arabidopsis”
Claim · model-written summary
Other pectic domains and glycoproteins did not change in the mutants.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- other pectic domains or in glycoproteinse0
- Direction
- no changee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunohistochemical analysis indicated a specific decrease in arabinan with no change in other pectic domains or in glycoproteins”
t0“Arabidopsis”
Claim · model-written summary
Mutant stem cellular structure was not altered.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- steme0
- Outcome
- cellular structuree0
- Direction
- not alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cellular structure of the stem was also not altered”
t0“Arabidopsis”
Claim · model-written summary
Rhamnogalacturonan I from mutant tissue had about 30% of wild-type arabinose.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- amount of arabinosee0
- Direction
- onlye0
- Value
- about 30%e0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolated rhamnogalacturonan I from mutant tissues contained only about 30% of the wild-type amount of arabinose”
t0“Arabidopsis”
Claim · model-written summary
Residual arabinan in mutant tissue was structurally similar to wild type.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- arabinan present in mutant tissuee0
- Qualifier
- structurally similare0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Linkage analysis showed that the small amount of arabinan present in mutant tissue was structurally similar to that of the wild type”
t0“Arabidopsis”
Claim · model-written summary
ARAD1 transformation fully complemented the mutant phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Transformation of mutant plants with the ARAD1 gene driven by the 35S promotere0
- Gene
- ARAD1e0
- Outcome
- full complementation of the phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation of mutant plants with the ARAD1 gene driven by the 35S promoter led to full complementation of the phenotype”
t0“Arabidopsis”
Claim · model-written summary
No ARAD1 transformant exceeded wild-type arabinan levels.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- arabinane0
- Direction
- none of the transformants had moree0
- Comparator
- wild-type levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“none of the transformants had more arabinan than the wild-type level”
t0“Arabidopsis”
Claim · model-written summary
The authors suggest ARAD1 is an arabinan alpha-1,5-arabinosyltransferase.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ARAD1e0
- Outcome
- arabinan alpha-1,5-arabinosyltransferasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The data suggest that ARAD1 is an arabinan alpha-1,5-arabinosyltransferase”
t0“Arabidopsis”