Abstract
Plants can sense and respond to mechanical stimuli, like animals. An early mechanism of mechanosensing and response is speculated to be governed by as-yet-unidentified sensory complexes containing a Ca(2+)-permeable, stretch-activated (SA) channel. However, the components or regulators of such complexes are poorly understood at the molecular level in plants. Here, we report the molecular identification of a plasma membrane protein (designated Mca1) that correlates Ca(2+) influx with mechanosensing in Arabidopsis thaliana. MCA1 cDNA was cloned by the functional complementation of lethality of a yeast mid1 mutant lacking a putative Ca(2+)-permeable SA channel component. Mca1 was localized to the yeast plasma membrane as an integral membrane protein and mediated Ca(2+) influx. Mca1 also increased [Ca(2+)](cyt) upon plasma membrane distortion in Arabidopsis. The growth of MCA1-overexpressing plants was impaired in a high-calcium but not a low-calcium medium. The primary roots of mca1-null plants failed to penetrate a harder agar medium from a softer one. These observations demonstrate that Mca1 plays a crucial role in a Ca(2+)-permeable SA channel system that leads to mechanosensing in Arabidopsis. We anticipate our findings to be a starting point for a deeper understanding of the molecular mechanisms of mechanotransduction in plants.
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Claims 6
Claim · model-written summary
MCA1 cDNA functionally complemented lethal yeast mid1 mutation.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- MCA1 cDNAe0
- Gene
- MCA1e0
- Outcome
- lethalitye0
- Comparator
- yeast mid1 mutante0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MCA1 cDNA was cloned by the functional complementation of lethality of a yeast mid1 mutant lacking a putative Ca(2+)-permeable SA channel component”
e1“in Arabidopsis thaliana”
Claim · model-written summary
Mca1 localized to the yeast plasma membrane as an integral membrane protein.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- Mca1e0
- Outcome
- localized to the yeast plasma membrane as an integral membrane proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mca1 was localized to the yeast plasma membrane as an integral membrane protein”
e1“in Arabidopsis thaliana”
Claim · model-written summary
Mca1 mediated calcium influx in yeast.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- Mca1e0
- Outcome
- Ca(2+) influxe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mca1 was localized to the yeast plasma membrane as an integral membrane protein and mediated Ca(2+) influx”
e1“in Arabidopsis thaliana”
Claim · model-written summary
Mca1 increased cytosolic calcium after plasma membrane distortion in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- plasma membrane distortione0
- Gene
- Mca1e0
- Outcome
- [Ca(2+)](cyt)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mca1 also increased [Ca(2+)](cyt) upon plasma membrane distortion in Arabidopsis”
Claim · model-written summary
MCA1 overexpression impaired plant growth in high-calcium medium.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- MCA1-overexpressing plantse0
- Treatment
- high-calciume0
- Outcome
- growthe0
- Direction
- impairede0
- Comparator
- low-calcium mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of MCA1-overexpressing plants was impaired in a high-calcium but not a low-calcium medium”
e1“in Arabidopsis thaliana”
Claim · model-written summary
mca1-null primary roots failed to penetrate harder agar from softer agar.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- mca1-null plantse0
- Organ / stage
- primary rootse0
- Outcome
- failed to penetrate a harder agar medium from a softer onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The primary roots of mca1-null plants failed to penetrate a harder agar medium from a softer one”
e1“in Arabidopsis thaliana”
The same questions in other papers
physical treatment · nutrient: content · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.