Abstract
The effect of the plant growth regulators kinetin, gibberellic acid (GA 3 ), and 2,4- dichlorophenoxyacetic acid (2,4-D), either alone or in combination with orange juice, on the in vitro growth of mesocarp and endocarp explants from 90–120 day-old Washington Navel Citrus sinensis orange fruits has been determined. Both fruit tissues formed callus. From endocarp 100% callus was formed in all cultures, even without growth regulators in the culture medium, while callus growth was lower from mesocarp. The addition of orange juice at a final concentration of 15% (v/v) to the basal medium without growth regulators increased the callus growth, specially that derived from endocarp. However, the high stimulant effect of orange juice observed on callus growth was markedly dependent on the addition of 2,4-dichlorophenoxyacetic acid, maximum callus growth from endocarp or mesocarp tissues being obtained with orange juice 15% (v/v) and 10 μM 2,4-D.
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Claims 5
Claim · model-written summary
Callus formed from endocarp explants in all cultures, even without growth regulators
- Taxon
- Citrus sinensis
Citrus sinensise0 - Organ / stage
- endocarpe1
- Outcome
- calluse1
- Value
- 100%e1
- Qualifier
- even without growth regulatorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washington Navel Citrus sinensis orange fruits”
e1“From endocarp 100% callus was formed in all cultures, even without growth regulators in the culture medium, while callus growth was lower from mesocarp”
Claim · model-written summary
Callus growth was lower from mesocarp than from endocarp explants
- Taxon
- Citrus sinensisCitrus sinensise0
- Organ / stage
- mesocarpe1
- Outcome
- callus growthe1
- Direction
- lowere1
- Comparator
- endocarpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washington Navel Citrus sinensis orange fruits”
e1“From endocarp 100% callus was formed in all cultures, even without growth regulators in the culture medium, while callus growth was lower from mesocarp”
Claim · model-written summary
Orange juice at 15% (v/v) increased callus growth in basal medium without growth regulators, especially from endocarp
- Taxon
- Citrus sinensisCitrus sinensise0
- Treatment
- addition of orange juice at a final concentration of 15% (v/v)e2
- Outcome
- callus growthe2
- Direction
- increasede2
- Qualifier
- specially that derived from endocarpe2
- Comparator
- basal medium without growth regulatorse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washington Navel Citrus sinensis orange fruits”
e2“The addition of orange juice at a final concentration of 15% (v/v) to the basal medium without growth regulators increased the callus growth, specially that derived from endocarp”
Claim · model-written summary
Maximum callus growth from endocarp or mesocarp was obtained with 15% (v/v) orange juice and 10 μM 2,4-D
- Taxon
- Citrus sinensisCitrus sinensise0
- Organ / stage
- endocarp or mesocarp tissuese3
- Treatment
- orange juice 15% (v/v) and 10 μM 2,4-De3
- Outcome
- callus growthe3
- Direction
- maximume3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washington Navel Citrus sinensis orange fruits”
e3“maximum callus growth from endocarp or mesocarp tissues being obtained with orange juice 15% (v/v) and 10 μM 2,4-D”
Claim · model-written summary
Stimulant effect of orange juice on callus growth was markedly dependent on 2,4-dichlorophenoxyacetic acid
- Taxon
- Citrus sinensisCitrus sinensise0
- Treatment
- the addition of 2,4-dichlorophenoxyacetic acide4
- Outcome
- callus growthe4
- Qualifier
- markedly dependente4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washington Navel Citrus sinensis orange fruits”
e4“However, the high stimulant effect of orange juice observed on callus growth was markedly dependent on the addition of 2,4-dichlorophenoxyacetic acid”
The same questions in other papers
2,4-D · growth · Citrus sinensis (sweet orange)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.