Abstract
This study was carried out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sour orange, Carrizo and Karna Khatta rootstocks. An apical meristem (1-2 mm) having 3-leaf primordial (0.3-0.5 mm) were taken from the axillary proliferated nodal segments. Finely excised meristems were placed on aseptically grown rootstocks making an inverted 'T' incision in vitro. Micro grafts were inoculated on MS media added with different Plant Growth Regulators, i.e. IAA, NAA and 2,4-D each under varied concentrations (control, 0.1 ppm, 0.5 ppm, 1.0 ppm , 1.5 ppm, 2.0 and 3.0 ppm). Highest grafts success (60%) was recorded when 13 days old Carrizo seedlings were used as rootstock. Among all the treatments, scion treated with 2,4-D at 3.0 mg/l exerted maximum positive effect on the success of STG (70.00%). Overall, Sweet orange cv. Blood Red, micro grafted on Carrizo rootstock culturing on liquid MS media containing 2, 4-D at 3.0 mg/L resulted maximum success and growth of grafts. Successful micrografts were hardened under controlled conditions and further shifted in insect proof net house to develop disease free mother progeny.
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Claims 4
Claim · model-written summary
Carrizo rootstock at 13 days gave the highest graft success of 60%.
- Taxon
- sweet orange
Citrus sinensist0 - Treatment
- 13 days old Carrizo seedlingse0
- Outcome
- grafts successe0
- Direction
- Higheste0
- Value
- 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest grafts success (60%) was recorded when 13 days old Carrizo seedlings were used as rootstock.”
t0“sweet orange (Citrus sinensis)”
Claim · model-written summary
Scion treatment with 2,4-D at 3.0 mg/l maximized shoot tip grafting success at 70.00%.
- Taxon
- sweet orangeCitrus sinensist0
- Organ / stage
- scione0
- Treatment
- 2,4-D at 3.0 mg/le0
- Outcome
- success of STGe0
- Direction
- maximume0
- Value
- 70.00%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“scion treated with 2,4-D at 3.0 mg/l exerted maximum positive effect on the success of STG (70.00%).”
t0“sweet orange (Citrus sinensis)”
Claim · model-written summary
Blood Red sweet orange grafted on Carrizo in liquid MS media with 2, 4-D at 3.0 mg/L had maximum graft success and growth.
- Taxon
- Sweet orangeCitrus sinensise0
- Material
- Blood Rede0
- Treatment
- micro grafted on Carrizo rootstock culturing on liquid MS media containing 2, 4-D at 3.0 mg/Le0
- Outcome
- success and growth of graftse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sweet orange cv. Blood Red, micro grafted on Carrizo rootstock culturing on liquid MS media containing 2, 4-D at 3.0 mg/L resulted maximum success and growth of grafts.”
Claim · model-written summary
Successful micrografts were hardened and moved to an insect proof net house for disease-free mother progeny.
- Taxon
- sweet orangeCitrus sinensist0
- Outcome
- disease free mother progenye0
- Setting
- insect proof net housee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful micrografts were hardened under controlled conditions and further shifted in insect proof net house to develop disease free mother progeny.”
t0“sweet orange (Citrus sinensis)”
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