Abstract
A NaCI-tolerant cell line which was selected from ovular callus of 'Shamouti' orange (Citrus sinensis L. Osbeck) proved to be a true cell line variant. This conclusion is based on the following observations. (a) Cells which have been removed from the selection pressure for at least four passages retain the same NaCI tolerance as do cells which are kept constantly on 0.2 molar NaCl. (b) Na+ and Cl uptake are considerably lower in salt-tolerant cells (R-10) than in salt-sensitive cells (L-5) at a given external NaCl concentration. (c) Growth of salt-tolerant cells is markedly suppressed upon replacement of NaCI by KCI, whereas the growth of salt-sensitive cells is only slightly affected. Accumulation of K+ and Cl accompanies the inhibition of growth. Experiments carried out with sodium and potassium sulfate suggest that the toxic effect is due to the accumulated Cl1. (d) Removal of Ca2 from the growth medium severely inhibits the growth of salt-tolerant cells in the presence of NaCl, while it has a minor effect on growth of salt-sensitive cells in the presence of NaCI. (e) Electron micrographs show that the salt-tolerant cells have very big vacuoles when exposed to salt, while the size of the vacuoles of the salt-sensitive cells does not change.
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Claims 7
Claim · model-written summary
The salt selected orange cell line remained tolerant after at least four passages without selection.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)
Citrus sinensise0 - Material
- Cellse1
- Outcome
- NaCI tolerancee1
- Direction
- samee1
- Comparator
- cells which are kept constantly on 0.2 molar NaCle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“Cells which have been removed from the selection pressure for at least four passages retain the same NaCI tolerance as do cells which are kept constantly on 0.2 molar NaCl”
Claim · model-written summary
Salt tolerant R-10 cells took up less Na+ and Cl than salt sensitive L-5 cells at the same NaCl concentration.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)Citrus sinensise0
- Material
- R-10e1
- Outcome
- Na+ and Cl uptakee1
- Direction
- lowere1
- Comparator
- L-5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“Na+ and Cl uptake are considerably lower in salt-tolerant cells (R-10) than in salt-sensitive cells (L-5) at a given external NaCl concentration”
Claim · model-written summary
Replacing NaCl with KCl markedly suppressed R-10 growth and only slightly affected L-5 growth.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)Citrus sinensise0
- Treatment
- replacement of NaCI by KCIe1
- Outcome
- Growth of salt-tolerant cellse1
- Direction
- suppressede1
- Comparator
- growth of salt-sensitive cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“Growth of salt-tolerant cells is markedly suppressed upon replacement of NaCI by KCI, whereas the growth of salt-sensitive cells is only slightly affected”
Claim · model-written summary
K+ and Cl accumulated alongside KCl associated growth inhibition.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)Citrus sinensise0
- Outcome
- Accumulation of K+ and Cle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“Accumulation of K+ and Cl accompanies the inhibition of growth”
Claim · model-written summary
Sulfate experiments suggested accumulated chloride caused the toxic effect.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)Citrus sinensise0
- Compound
- accumulated Cl1e1
- Outcome
- toxic effecte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“Experiments carried out with sodium and potassium sulfate suggest that the toxic effect is due to the accumulated Cl1”
Claim · model-written summary
Removing Ca2 from medium severely inhibited salt tolerant cell growth with NaCl, but had a minor effect on salt sensitive cells.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)Citrus sinensise0
- Treatment
- Removal of Ca2 from the growth mediume1
- Outcome
- growth of salt-tolerant cellse1
- Direction
- inhibitse1
- Qualifier
- in the presence of NaCle1
- Comparator
- growth of salt-sensitive cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“Removal of Ca2 from the growth medium severely inhibits the growth of salt-tolerant cells in the presence of NaCl, while it has a minor effect on growth of salt-sensitive cells in the presence of NaCI”
Claim · model-written summary
Salt exposed tolerant cells had very large vacuoles, whereas sensitive cell vacuoles did not change size.
- Taxon
- 'Shamouti' orange (Citrus sinensis L. Osbeck)Citrus sinensise0
- Outcome
- very big vacuolese1
- Comparator
- salt-sensitive cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'Shamouti' orange (Citrus sinensis L. Osbeck)”
e1“salt-tolerant cells have very big vacuoles when exposed to salt, while the size of the vacuoles of the salt-sensitive cells does not change”
The same questions in other papers
in vitro · growth · Citrus sinensis (sweet orange)Positive and negative results
1 paper in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.