Abstract
Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7”. Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.
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Claims 9
Claim · model-written summary
ARAhPR10 was isolated and characterized as a new PR10 family putative resistance gene.
- Taxon
- Peanut
Arachis hypogaeat0 - Gene
- ARAhPR10e0
- Outcome
- isolated and characterizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized”
t0“Peanut Seeds”
Claim · model-written summary
Pre-harvest A. flavus infection induced ARAhPR10 expression.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- pre-harvested A. flavus infectione0
- Interactor
- A. flavus
Aspergillus flavuse0pathogen - Gene
- ARAhPR10e0
- Outcome
- expression of ARAhPR10e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ARAhPR10 was induced by pre-harvested A. flavus infection”
t0“Peanut Seeds”
Claim · model-written summary
ARAhPR10 expression did not differ significantly between resistant GT-C20 and susceptible Yueyou 7.
- Taxon
- PeanutArachis hypogaeat0
- Material
- resistant genotype “GT-C20”e0
- Outcome
- differencee0
- Direction
- no significant differencee0
- Comparator
- susceptible genotype “Yueyou 7”e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no significant difference was observed between resistant genotype “GT-C20” and susceptible genotype “Yueyou 7””
t0“Peanut Seeds”
Claim · model-written summary
Seven transgenic peanut lines expressing ARAhPR10 were obtained.
- Taxon
- peanutArachis hypogaeae0
- Gene
- ARAhPR10e0
- Outcome
- transgenic peanut linese0
- Value
- Sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained”
Claim · model-written summary
ARAhPR10 expression was significantly higher in transgenic seeds than wild type.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- transgenic linese0
- Gene
- ARAhPR10e0
- Outcome
- expression level of the ARAhPR10e0
- Direction
- highere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type”
t0“Peanut Seeds”
Claim · model-written summary
A. flavus infection was significantly lower in transgenic seeds than wild type.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- transgenic linese0
- Interactor
- A. flavusAspergillus flavuse0pathogen
- Outcome
- A. flavus infectione0
- Direction
- lowere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type”
t0“Peanut Seeds”
Claim · model-written summary
Aflatoxin content was significantly lower in transgenic seeds than wild type.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- transgenic linese0
- Outcome
- aflatoxin contente0
- Direction
- lowere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type”
t0“Peanut Seeds”
Claim · model-written summary
ARAhPR10 transcript expression and seed aflatoxin production were significantly negatively correlated.
- Taxon
- PeanutArachis hypogaeat0
- Gene
- ARAhPR10e0
- Outcome
- seeds aflatoxin productione0
- Direction
- negative correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed”
t0“Peanut Seeds”
Claim · model-written summary
Authors suggest ARAhPR10 expression has an important role in peanut resistance to A. flavus infection and aflatoxin production.
- Taxon
- peanutArachis hypogaeae0
- Interactor
- A. flavusAspergillus flavuse0pathogen
- Gene
- ARAhPR10e0
- Outcome
- peanut host resistance to A. flavus infection and aflatoxin producinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing”
The same questions in other papers
Aspergillus flavus · molecular: expression · Arachis hypogaea (peanut)Positive and negative results
2 in the opposite direction
- positive
- positive
infection · molecular: expression · Arachis hypogaea (peanut)Positive and negative results
5 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.