Abstract
BACKGROUND: Germin-like superfamily members are ubiquitously expressed in various plant species and play important roles in plant development and defense. Although several GLPs have been identified in peanut (Arachis hypogaea L.), their roles in development and defense remain unknown. In this research, we study the spatiotemporal expression of AhGLPs in peanut and their functions in plant defense. RESULTS: We have identified three new AhGLP members (AhGLP3b, AhGLP5b and AhGLP7b) that have distinct but very closely related DNA sequences. The spatial and temporal expression profiles revealed that each peanut GLP gene has its distinct expression pattern in various tissues and developmental stages. This suggests that these genes all have their distinct roles in peanut development. Subcellular location analysis demonstrated that AhGLP2 and 5 undergo a protein transport process after synthesis. The expression of all AhGLPs increased in responding to Aspergillus flavus infection, suggesting AhGLPs' ubiquitous roles in defense to A. flavus. Each AhGLP gene had its unique response to various abiotic stresses (including salt, H2O2 stress and wound), biotic stresses (including leaf spot, mosaic and rust) and plant hormone stimulations (including SA and ABA treatments). These results indicate that AhGLPs have their distinct roles in plant defense. Moreover, in vivo study of AhGLP transgenic Arabidopsis showed that both AhGLP2 and 3 had salt tolerance, which made transgenic Arabidopsis grow well under 100 mM NaCl stress. CONCLUSIONS: For the first time, our study analyzes the AhGLP gene expression profiles in peanut and reveals their roles under various stresses. These results provide an insight into the developmental and defensive roles of GLP gene family in peanut.
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Claims 8
Claim · model-written summary
Three new AhGLP members (AhGLP3b, AhGLP5b and AhGLP7b) were identified in peanut.
- Taxon
- peanut
Arachis hypogaeat0 - Gene
- AhGLP3b, AhGLP5b and AhGLP7be1
- Outcome
- three new AhGLP memberse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e1“We have identified three new AhGLP members (AhGLP3b, AhGLP5b and AhGLP7b)”
Claim · model-written summary
Each peanut GLP gene has its distinct expression pattern in various tissues and developmental stages.
- Taxon
- peanutArachis hypogaeae2
- Organ / stage
- various tissues and developmental stagese2
- Gene
- peanut GLP genee2
- Outcome
- distinct expression patterne2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e2“each peanut GLP gene has its distinct expression pattern in various tissues and developmental stages”
Claim · model-written summary
AhGLP2 and AhGLP5 undergo a protein transport process after synthesis.
- Taxon
- peanutArachis hypogaeat0
- Gene
- AhGLP2 and 5e3
- Outcome
- undergo a protein transport process after synthesise3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e3“Subcellular location analysis demonstrated that AhGLP2 and 5 undergo a protein transport process after synthesis”
Claim · model-written summary
Expression of all AhGLPs increased in response to Aspergillus flavus infection in peanut.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- Aspergillus flavus infectione4
- Interactor
- Aspergillus flavus
Aspergillus flavuse4pathogen - Gene
- AhGLPse4
- Outcome
- expression of all AhGLPse4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e4“The expression of all AhGLPs increased in responding to Aspergillus flavus infection”
Claim · model-written summary
Each AhGLP gene responded uniquely to salt, H2O2 and wound stress in peanut.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- various abiotic stresses (including salt, H2O2 stress and wound)e5
- Gene
- AhGLP genee5
- Outcome
- unique responsee5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e5“Each AhGLP gene had its unique response to various abiotic stresses (including salt, H2O2 stress and wound)”
Claim · model-written summary
Each AhGLP gene responded uniquely to leaf spot, mosaic and rust in peanut.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- biotic stresses (including leaf spot, mosaic and rust)e6
- Gene
- AhGLP genee6
- Outcome
- unique responsee6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e6“Each AhGLP gene had its unique response to various abiotic stresses (including salt, H2O2 stress and wound), biotic stresses (including leaf spot, mosaic and rust)”
Claim · model-written summary
Each AhGLP gene responded uniquely to SA and ABA treatments in peanut.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- plant hormone stimulations (including SA and ABA treatments)e7
- Gene
- AhGLP genee7
- Outcome
- unique responsee7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e7“Each AhGLP gene had its unique response to various abiotic stresses (including salt, H2O2 stress and wound), biotic stresses (including leaf spot, mosaic and rust) and plant hormone stimulations (including SA and ABA treatments)”
Claim · model-written summary
AhGLP2 and AhGLP3 conferred salt tolerance on transgenic Arabidopsis grown under 100 mM NaCl.
- Taxon
- peanutArachis hypogaeat0
- Material
- transgenic Arabidopsise0
- Treatment
- 100 mM NaCl stresse0
- Gene
- AhGLP2 and 3e0
- Outcome
- salt tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut Germin-Like Proteins”
e0“both AhGLP2 and 3 had salt tolerance, which made transgenic Arabidopsis grow well under 100 mM NaCl stress”
The same questions in other papers
Aspergillus flavus · molecular: expression · Arachis hypogaea (peanut)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
infection · molecular: expression · Arachis hypogaea (peanut)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.