Abstract
Although steroid hormones are important for animal development, the physiological role of plant steroids is unknown. The Arabidopsis DET2 gene encodes a protein that shares significant sequence identity with mammalian steroid 5 alpha-reductases. A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2 and leads to defects in light-regulated development that can be ameliorated by application of a plant steroid, brassinolide. Thus, DET2 may encode a reductase in the brassinolide biosynthetic pathway, and brassinosteroids may constitute a distinct class of phytohormones with an important role in light-regulated development of higher plants.
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Claims 6
Claim · model-written summary
DET2 protein shares significant sequence identity with mammalian steroid 5 alpha reductases.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- DET2e0
- Outcome
- significant sequence identity with mammalian steroid 5 alpha-reductasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis DET2 gene encodes a protein that shares significant sequence identity with mammalian steroid 5 alpha-reductases”
Claim · model-written summary
Mutation of glutamate 204 abolishes DET2 activity in vivo.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- A mutation of glutamate 204e0
- Gene
- DET2e0
- Outcome
- in vivo activity of DET2e0
- Direction
- abolishese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2”
t0“Arabidopsis”
Claim · model-written summary
The DET2 mutation causes defects in light regulated development.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- A mutation of glutamate 204e0
- Gene
- DET2e0
- Outcome
- defects in light-regulated developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2 and leads to defects in light-regulated development”
t0“Arabidopsis”
Claim · model-written summary
Brassinolide application ameliorates light regulated developmental defects.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- application of a plant steroid, brassinolidee0
- Outcome
- defects in light-regulated developmente0
- Direction
- amelioratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“defects in light-regulated development that can be ameliorated by application of a plant steroid, brassinolide”
t0“Arabidopsis”
Claim · model-written summary
DET2 may encode a reductase in the brassinolide biosynthetic pathway.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DET2e0
- Outcome
- brassinolide biosynthetic pathwaye0
- Qualifier
- maye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DET2 may encode a reductase in the brassinolide biosynthetic pathway”
t0“Arabidopsis”
Claim · model-written summary
Brassinosteroids may be a phytohormone class important for light regulated development.
- Taxon
- higher plantsEmbryophytae0
- Compound
- brassinosteroidse0
- Outcome
- light-regulated developmente0
- Qualifier
- maye0
Verbatim
e0“brassinosteroids may constitute a distinct class of phytohormones with an important role in light-regulated development of higher plants”
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See all 234 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.