Abstract
We have examined the seed germination in Arabidopsis thaliana of wild type (wt), and phytochrome A (PhyA)- and B (PhyB)-mutants in terms of incubation time and environmental light effects. Seed germination of the wt and PhyA-null mutant (phyA) was photoreversibly regulated by red and far-red lights of 10-1,000 micromol m-2 when incubated in darkness for 1-14 hr, but no germination occurred in PhyB-null mutant (phyB). When wt seeds and the phyB mutant seeds were incubated in darkness for 48 hr, they synthesized PhyA during dark incubation and germinated upon exposure to red light of 1-100 nmol m-2 and far-red light of 0.5-10 micromol m-2, whereas the phyA mutant showed no such response. The results indicate that the seed germination is regulated by PhyA and PhyB but not by other phytochromes, and the effects of PhyA and PhyB are separable in this assay. We determined action spectra separately for PhyA- and PhyB-specific induction of seed germination at Okazaki large spectrograph. Action spectra for the PhyA response show that monochromatic 300-780 nm lights of very low fluence induced the germination, and this induction was not photoreversible in the range examined. Action spectra for the PhyB response show that germination was photoreversibly regulated by alternate irradiations with light of 0.01-1 mmol m-2 at wavelengths of 540-690 nm and 695-780 nm. The present work clearly demonstrated that PhyA photoirreversibly triggers the germination upon irradiations with ultraviolet, visible and far-red light of very low fluence, while PhyB controls the photoreversible effects of low fluence.
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Claims 9
Claim · model-written summary
The phyB-null mutant seeds did not germinate under the tested dark incubation and light conditions.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- PhyB-null mutante1
- Outcome
- seed germinatione1
- Qualifier
- no germination occurrede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e1“Seed germination of the wt and PhyA-null mutant (phyA) was photoreversibly regulated by red and far-red lights of 10-1,000 micromol m-2 when incubated in darkness for 1-14 hr, but no germination occurred in PhyB-null mutant (phyB)”
Claim · model-written summary
Seed germination of wild type and phyA-null seeds was photoreversibly regulated by red and far-red light of 10-1,000 micromol m-2.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- PhyA-null mutante1
- Treatment
- red and far-red lights of 10-1,000 micromol m-2e1
- Outcome
- Seed germinatione1
- Qualifier
- when incubated in darkness for 1-14 hre1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e1“Seed germination of the wt and PhyA-null mutant (phyA) was photoreversibly regulated by red and far-red lights of 10-1,000 micromol m-2 when incubated in darkness for 1-14 hr, but no germination occurred in PhyB-null mutant (phyB)”
Claim · model-written summary
Phytochrome B mutant seeds that were incubated in darkness for 48 hr synthesized PhyA and germinated under red and far-red light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- phyB mutant seedse2
- Treatment
- red light of 1-100 nmol m-2 and far-red light of 0.5-10 micromol m-2e2
- Outcome
- germinatede2
- Qualifier
- incubated in darkness for 48 hre2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e2“When wt seeds and the phyB mutant seeds were incubated in darkness for 48 hr, they synthesized PhyA during dark incubation and germinated upon exposure to red light of 1-100 nmol m-2 and far-red light of 0.5-10 micromol m-2, whereas the phyA mutant showed no such response”
Claim · model-written summary
The phyA-null mutant showed no such germination response to red and far-red light after 48 hr dark incubation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- phyA mutante2
- Outcome
- no such responsee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e2“When wt seeds and the phyB mutant seeds were incubated in darkness for 48 hr, they synthesized PhyA during dark incubation and germinated upon exposure to red light of 1-100 nmol m-2 and far-red light of 0.5-10 micromol m-2, whereas the phyA mutant showed no such response”
Claim · model-written summary
Monochromatic 300-780 nm light of very low fluence induced PhyA-dependent germination that was not photoreversible.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- monochromatic 300-780 nm lights of very low fluencee4
- Gene
- PhyAe4
- Outcome
- the germinatione4
- Qualifier
- was not photoreversiblee4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e4“Action spectra for the PhyA response show that monochromatic 300-780 nm lights of very low fluence induced the germination, and this induction was not photoreversible in the range examined”
Claim · model-written summary
Germination under alternating 540-690 nm and 695-780 nm irradiation at 0.01-1 mmol m-2 was photoreversibly regulated by PhyB.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- alternate irradiations with light of 0.01-1 mmol m-2 at wavelengths of 540-690 nm and 695-780 nme5
- Gene
- PhyBe5
- Outcome
- germinatione5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e5“Action spectra for the PhyB response show that germination was photoreversibly regulated by alternate irradiations with light of 0.01-1 mmol m-2 at wavelengths of 540-690 nm and 695-780 nm”
Claim · model-written summary
PhyA photoirreversibly triggers seed germination under UV, visible and far-red light of very low fluence.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- PhyAe6
- Outcome
- the germinatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e6“The present work clearly demonstrated that PhyA photoirreversibly triggers the germination upon irradiations with ultraviolet, visible and far-red light of very low fluence, while PhyB controls the photoreversible effects of low fluence”
Claim · model-written summary
PhyB controls the photoreversible effects of low fluence on seed germination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- PhyBe6
- Outcome
- photoreversible effectse6
- Qualifier
- of low fluencee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e6“The present work clearly demonstrated that PhyA photoirreversibly triggers the germination upon irradiations with ultraviolet, visible and far-red light of very low fluence, while PhyB controls the photoreversible effects of low fluence”
Claim · model-written summary
Seed germination is regulated by PhyA and PhyB but not by other phytochromes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- PhyA and PhyBe3
- Outcome
- seed germinatione3
- Qualifier
- but not by other phytochromese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have examined the seed germination in Arabidopsis thaliana of wild type (wt)”
e3“The results indicate that the seed germination is regulated by PhyA and PhyB but not by other phytochromes”
The same questions in other papers
light · growth · Arabidopsis thaliana (thale cress)Results differ
29 papers in the same direction as this paper · 14 in the opposite direction
- negative
- positive
- negative
40 papers more in this group
See all 44 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.