Abstract
Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level. The GS level decrease was preceded by a temporary increase in NO2- content: nitrite content maximum was observed after 6 h exposure, GS level was depressed most after 12 h. At this time, GS level also significantly decreased by 0.3 and 0.4 mM NO2-, but the effect of these concentrations was only slight and not significant after 18 h exposure. By contrast, GS level in roots precultivated for 24 h in solutions containing 2 mM NO2- did not fully recover even after further 24 h cultivation in nutrient solutions lacking NO2-. The inhibitory effect of NO2-on GS level was stressed by lack of sugar in nutrient solutions. Nitrite did not affect GS activity (transferase reaction)in vitro. The decrease in GS level due to exposure to No2- was accompanied by KH3 excretion to nutrient solution.
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Claims 9
Claim · model-written summary
Exposure to 0.5 and 2.0 mM nitrite temporarily decreased GS level.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- rootse0
- Treatment
- 0.5 and 2.0 mM nitritee0
- Outcome
- glutamine synthetase (GS) levele0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
Claim · model-written summary
Nitrite content temporarily increased before GS level declined, peaking at six hours.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- NO2- contente0
- Direction
- increasee0
- Qualifier
- after 6 h exposuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GS level decrease was preceded by a temporary increase in NO2- content: nitrite content maximum was observed after 6 h exposure”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
Claim · model-written summary
GS level was most depressed after 12 hours.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- GS levele0
- Direction
- depressed moste0
- Qualifier
- after 12 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GS level was depressed most after 12 h”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
Claim · model-written summary
At 12 hours, 0.3 and 0.4 mM nitrite significantly reduced GS level.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- 0.3 and 0.4 mM NO2-e0
- Outcome
- GS levele0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At this time, GS level also significantly decreased by 0.3 and 0.4 mM NO2-”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
Claim · model-written summary
The 0.3 and 0.4 mM nitrite effects were slight and nonsignificant after 18 hours.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- 0.3 and 0.4 mM NO2-e2
- Outcome
- GS levele2
- Qualifier
- after 18 h exposuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effect of these concentrations was only slight and not significant after 18 h exposure”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
e2“At this time, GS level also significantly decreased by 0.3 and 0.4 mM NO2-”
Claim · model-written summary
GS level did not fully recover after 24 hours without nitrite following 24 hours at 2 mM nitrite.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- 2 mM NO2-e0
- Outcome
- GS levele0
- Direction
- did not fully recovere0
- Comparator
- nutrient solutions lacking NO2-e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GS level in roots precultivated for 24 h in solutions containing 2 mM NO2- did not fully recover even after further 24 h cultivation in nutrient solutions lacking NO2-”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
Claim · model-written summary
Lack of sugar intensified nitrite inhibition of GS level.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- lack of sugare0
- Outcome
- inhibitory effect of NO2-on GS levele0
- Direction
- stressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibitory effect of NO2-on GS level was stressed by lack of sugar in nutrient solutions”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
Claim · model-written summary
Nitrite did not affect GS transferase activity in vitro.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- Nitritee0
- Outcome
- GS activity (transferase reaction)e0
- Direction
- did not affecte0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrite did not affect GS activity (transferase reaction)in vitro”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
Claim · model-written summary
The nitrite induced decrease in GS level accompanied KH3 excretion.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- No2-e0
- Outcome
- KH3 excretion to nutrient solutione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease in GS level due to exposure to No2- was accompanied by KH3 excretion to nutrient solution”
t0“The effect of nitrite on glutamine synthetase level in isolatedPisutn sativutn L. roots”
e1“Exposure of isolated pea roots to 0.5 and 2.0 mM nitrite resulted in a temporary decrease in glutamine synthetase (GS) level”
The same questions in other papers
nitrite · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.