Abstract
Oxygen deprivation leads to changes in mitochondrial morphology and impaired flow of reducing equivalents through the electron transport chain. The extent of these changes depends on the duration and severity of the treatment as well as on the species and cell type. Nitrate is known to ameliorate these effects in some instances, but it is possible that it is nitrite, rather than nitrate, that is the key to the mechanism. To test this, mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite. The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation. It also resulted in higher levels and activities of complex I and the supercomplex I + III2. It is concluded that nitrite has an important role in maintaining mitochondrial function under hypoxia, and that it achieves this through the reduction of nitrite to nitric oxide by the mitochondrial electron transport chain.
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Claims 8
Claim · model-written summary
Nitrite supply under hypoxia led to nitric oxide production in isolated pea root mitochondria.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- nitric oxide productione1
- Direction
- increaseinferrede1
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation”
Claim · model-written summary
Nitrite supply under hypoxia improved mitochondrial integrity in pea roots.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- mitochondrial integritye1
- Direction
- improvede1
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation”
Claim · model-written summary
Nitrite supply under hypoxia improved energization of the inner mitochondrial membrane.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- energization of the inner mitochondrial membranee1
- Direction
- improvede1
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation”
Claim · model-written summary
Nitrite supply under hypoxia increased ATP synthesis by pea root mitochondria.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- ATP synthesise1
- Direction
- increasede1
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation”
Claim · model-written summary
Nitrite supply under hypoxia decreased production of reactive oxygen species.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- production of reactive oxygen speciese1
- Direction
- decreasede1
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation”
Claim · model-written summary
Nitrite supply under hypoxia decreased lipid peroxidation.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- lipid peroxidatione1
- Direction
- decreasede1
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia led to nitric oxide production, improved mitochondrial integrity, improved energization of the inner mitochondrial membrane, increased ATP synthesis, decreased production of reactive oxygen species and decreased lipid peroxidation”
Claim · model-written summary
Nitrite under hypoxia also resulted in higher levels and activities of complex I and the supercomplex I + III2.
- Taxon
- peaLathyrus oleraceuse0
- Organ / stage
- rootse0
- Treatment
- supply of nitrite under hypoxiae1
- Outcome
- levels and activities of complex Ie2
- Direction
- highere2
- Comparator
- absence of 0.5 mM nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“The supply of nitrite under hypoxia”
e2“It also resulted in higher levels and activities of complex I and the supercomplex I + III2”
Claim · model-written summary
The authors conclude that nitrite helps maintain mitochondrial function under hypoxia by being reduced to nitric oxide by the mitochondrial electron transport chain.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- nitritee1
- Outcome
- mitochondrial functione1
- Qualifier
- reduction of nitrite to nitric oxide by the mitochondrial electron transport chaine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mitochondria were isolated from 21-day-old pea (Pisum sativum) roots and incubated for 90 min under normoxia or hypoxia in the presence or absence of 0.5 mM nitrite”
e1“nitrite has an important role in maintaining mitochondrial function under hypoxia, and that it achieves this through the reduction of nitrite to nitric oxide by the mitochondrial electron transport chain”
The same questions in other papers
nitrite · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
1 in the opposite direction
- negative
flooding · physiology: oxidative · Lathyrus oleraceus (pea)Positive and negative results
2 in the opposite direction
- negative
- negative
flooding · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
flooding · yield · Lathyrus oleraceus (pea)Positive and negative results
4 in the opposite direction
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.