Abstract
MicroRNAs are recognized as important post-transcriptional regulators in plants. Information about the roles of miRNAs in common bean (Phaseolus vulgaris L.), an agronomically important legume, is yet scant. The objective of this work was to functionally characterize the conserved miRNA: miR398b and its target Cu/Zn Superoxide Dismutase 1 (CSD1) in common bean. We experimentally validated a novel miR398 target: the stress up-regulated Nodulin 19 (Nod19). Expression analysis of miR398b and target genes -CSD1 and Nod19- in bean roots, nodules and leaves, indicated their role in copper (Cu) homeostasis. In bean plants under Cu toxicity miR398b was decreased and Nod19 and CSD1, that participates in reactive oxygen species (ROS) detoxification, were up-regulated. The opposite regulation was observed in Cu deficient bean plants; lower levels of CSD1 would allow Cu delivery to essential Cu-containing proteins. Composite common bean plants with transgenic roots over-expressing miR398 showed ca. 20-fold higher mature miR398b and almost negligible target transcript levels as well as increased anthocyanin content and expression of Cu-stress responsive genes, when subjected to Cu deficiency. The down-regulation of miR398b with the consequent up-regulation of its targets was observed in common bean roots during the oxidative burst resulting from short-time exposure to high Cu. A similar response occurred at early stage of bean roots inoculated with Rhizobium tropici, where an increase in ROS was observed. In addition, the miR398b down-regulation and an increase in CSD1 and Nod19 were observed in bean leaves challenged with Sclerotinia scleortiorum fungal pathogen. Transient over-expression of miR398b in Nicotiana benthamiana leaves infected with S. sclerotiorum resulted in enhanced fungal lesions. We conclude that the miR398b-mediated up-regulation of CSD and Nod19 is relevant for common bean plants to cope with oxidative stress generated in abiotic and biotic stresses.
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Claims 17
Claim · model-written summary
Under copper toxicity, miR398b expression decreased in bean plants.
Not reportedComparator
Verbatim
e1“In bean plants under Cu toxicity miR398b was decreased and Nod19 and CSD1, that participates in reactive oxygen species (ROS) detoxification, were up-regulated”
Claim · model-written summary
Under copper toxicity, Nod19 and CSD1 were up-regulated in bean plants.
- Taxon
- bean plantse1
- Treatment
- Cu toxicitye1
- Gene
- Nod19 and CSD1e1
- Outcome
- Nod19 and CSD1e1
- Direction
- up-regulatede1
Not reportedComparator
Verbatim
e1“In bean plants under Cu toxicity miR398b was decreased and Nod19 and CSD1, that participates in reactive oxygen species (ROS) detoxification, were up-regulated”
Claim · model-written summary
Under copper deficiency, CSD1 levels were lower in bean plants.
Not reportedComparator
Verbatim
e2“The opposite regulation was observed in Cu deficient bean plants; lower levels of CSD1 would allow Cu delivery to essential Cu-containing proteins”
Claim · model-written summary
Under copper deficiency, miR398b regulation ran opposite to that under copper toxicity, with higher miR398b levels.
Not reportedComparator
Verbatim
e2“The opposite regulation was observed in Cu deficient bean plants; lower levels of CSD1 would allow Cu delivery to essential Cu-containing proteins”
Claim · model-written summary
Expression of miR398b and CSD1 and Nod19 indicates a role of miR398b in copper homeostasis of bean.
Verbatim
e3“Expression analysis of miR398b and target genes -CSD1 and Nod19- in bean roots, nodules and leaves, indicated their role in copper (Cu) homeostasis”
Claim · model-written summary
A novel miR398 target, Nodulin 19 (Nod19), was experimentally validated in common bean.
- Taxon
- Common Bean
Phaseolus vulgarist0 - Gene
- Nodulin 19 (Nod19)e4
- Outcome
- validated a novel miR398 targete4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Common Bean”
e4“We experimentally validated a novel miR398 target: the stress up-regulated Nodulin 19 (Nod19)”
Claim · model-written summary
Over-expressing miR398 in composite common bean roots under Cu deficiency gave about 20-fold higher mature miR398b.
- Taxon
- common bean plantsPhaseolus vulgarise5
- Treatment
- over-expressing miR398e5
- Outcome
- mature miR398be5
- Direction
- highere5
- Value
- ca. 20-folde5
- Qualifier
- when subjected to Cu deficiencye5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Composite common bean plants with transgenic roots over-expressing miR398 showed ca. 20-fold higher mature miR398b and almost negligible target transcript levels as well as increased anthocyanin content and expression of Cu-stress responsive genes, when subjected to Cu deficiency”
Claim · model-written summary
Over-expressing miR398 under Cu deficiency gave almost negligible target transcript levels.
- Taxon
- common bean plantsPhaseolus vulgarise5
- Treatment
- over-expressing miR398e5
- Outcome
- target transcript levelse5
- Direction
- decreaseinferrede5
- Qualifier
- when subjected to Cu deficiencye5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Composite common bean plants with transgenic roots over-expressing miR398 showed ca. 20-fold higher mature miR398b and almost negligible target transcript levels as well as increased anthocyanin content and expression of Cu-stress responsive genes, when subjected to Cu deficiency”
Claim · model-written summary
Over-expressing miR398 under Cu deficiency increased anthocyanin content.
- Taxon
- common bean plantsPhaseolus vulgarise5
- Treatment
- over-expressing miR398e5
- Outcome
- anthocyanin contente5
- Direction
- increasede5
- Qualifier
- when subjected to Cu deficiencye5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Composite common bean plants with transgenic roots over-expressing miR398 showed ca. 20-fold higher mature miR398b and almost negligible target transcript levels as well as increased anthocyanin content and expression of Cu-stress responsive genes, when subjected to Cu deficiency”
Claim · model-written summary
Over-expressing miR398 under Cu deficiency increased expression of Cu-stress responsive genes.
- Taxon
- common bean plantsPhaseolus vulgarise5
- Treatment
- over-expressing miR398e5
- Outcome
- expression of Cu-stress responsive genese5
- Direction
- increasede5
- Qualifier
- when subjected to Cu deficiencye5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Composite common bean plants with transgenic roots over-expressing miR398 showed ca. 20-fold higher mature miR398b and almost negligible target transcript levels as well as increased anthocyanin content and expression of Cu-stress responsive genes, when subjected to Cu deficiency”
Claim · model-written summary
During the oxidative burst from short-time high Cu exposure, miR398b was down-regulated in common bean roots.
- Taxon
- common beanPhaseolus vulgarise6
- Organ / stage
- rootse6
- Treatment
- short-time exposure to high Cue6
- Outcome
- miR398be6
- Direction
- down-regulatione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The down-regulation of miR398b with the consequent up-regulation of its targets was observed in common bean roots during the oxidative burst resulting from short-time exposure to high Cu”
Claim · model-written summary
During the oxidative burst from short-time high Cu exposure, the targets of miR398b were up-regulated in common bean roots.
- Taxon
- common beanPhaseolus vulgarise6
- Organ / stage
- rootse6
- Treatment
- short-time exposure to high Cue6
- Outcome
- up-regulation of its targetse6
- Direction
- up-regulatione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The down-regulation of miR398b with the consequent up-regulation of its targets was observed in common bean roots during the oxidative burst resulting from short-time exposure to high Cu”
Claim · model-written summary
Inoculation of bean roots with Rhizobium tropici increased ROS at an early stage.
- Taxon
- beane7
- Organ / stage
- early stage of bean rootse7
- Treatment
- inoculated with Rhizobium tropicie7
- Interactor
- Rhizobium tropici
Martinezella tropicie7symbiont - Outcome
- ROSe7
- Direction
- increasee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A similar response occurred at early stage of bean roots inoculated with Rhizobium tropici, where an increase in ROS was observed”
Claim · model-written summary
In bean leaves challenged with Sclerotinia scleortiorum, miR398b was down-regulated.
- Taxon
- beane0
- Organ / stage
- leavese0
- Treatment
- challenged with Sclerotinia scleortiorume0
- Interactor
- Sclerotinia scleortiorume0pathogen
- Outcome
- miR398be0
- Direction
- down-regulatione0
Not reportedComparator
Verbatim
e0“the miR398b down-regulation and an increase in CSD1 and Nod19 were observed in bean leaves challenged with Sclerotinia scleortiorum fungal pathogen”
Claim · model-written summary
In bean leaves challenged with Sclerotinia scleortiorum, CSD1 and Nod19 increased.
- Taxon
- beane0
- Organ / stage
- leavese0
- Treatment
- challenged with Sclerotinia scleortiorume0
- Interactor
- Sclerotinia scleortiorume0pathogen
- Gene
- CSD1 and Nod19e0
- Outcome
- CSD1 and Nod19e0
- Direction
- increasee0
Not reportedComparator
Verbatim
e0“the miR398b down-regulation and an increase in CSD1 and Nod19 were observed in bean leaves challenged with Sclerotinia scleortiorum fungal pathogen”
Claim · model-written summary
Transient over-expression of miR398b in Nicotiana benthamiana leaves infected with S. sclerotiorum enhanced fungal lesions.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Transient over-expression of miR398be0
- Interactor
- S. sclerotiorum
Sclerotinia sclerotiorume0pathogen - Outcome
- fungal lesionse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient over-expression of miR398b in Nicotiana benthamiana leaves infected with S. sclerotiorum resulted in enhanced fungal lesions”
Claim · model-written summary
The miR398b-mediated up-regulation of CSD and Nod19 is relevant for common bean to cope with oxidative stress from abiotic and biotic stresses.
- Taxon
- common beanPhaseolus vulgarise0
- Gene
- CSD and Nod19e0
- Outcome
- cope with oxidative stresse0
- Qualifier
- miR398b-mediated up-regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We conclude that the miR398b-mediated up-regulation of CSD and Nod19 is relevant for common bean plants to cope with oxidative stress generated in abiotic and biotic stresses”
The same questions in other papers
Martinezella tropici · physiology: oxidative · Phaseolus vulgaris (common bean)Positive and negative results
1 in the opposite direction
- positive
inoculation · physiology: oxidative · Phaseolus vulgaris (common bean)Positive and negative results
6 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.