Abstract
Plants growing under natural conditions are constantly exposed to various stress factors, which can restrain their productivity and limit yields. This paper deals with the effect of long- and short-term osmotic stress followed by recovery on the formation of polysomes and their stability during germination of pea ( Pisum sativum L.) seeds. By isolating polysomes, it is possible to obtain an index which evidences the ability of tissues to synthesize proteins. Changes in the distribution of polysomes often precede measurable changes in amounts of proteins. Under osmotic stress, the dominant population of polysomes was the population of free polysomes (FP). The share of membrane-bound polysomes (MBP) and cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP) in the total fraction of ribosomes increased under intensive (−1.0 and −1.5 MPa) osmotic stress. These results can suggest that the bound forms of polysomes play an important role in the synthesis of stress proteins. In addition, the stability of polysomes isolated from pea early seedlings growing under unstressed control and osmotic stress conditions was tested. It turned out that polysomes formed under osmotic stress conditions (especially the CMBP) were more resistant to the activity of exogenous ribonucleases than the polysomes in the control samples. Under stress conditions it is highly likely that ribosomes become more densely packed on mRNA thus making it more resistant to ribonuclease. This is just one of the many mechanisms regulating stability of mRNA.
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Claims 5
Claim · model-written summary
Free polysomes were the dominant population during pea osmotic stress.
- Taxon
- pea
Lathyrus oleraceust0 - Treatment
- osmotic stresse0
- Outcome
- dominant population of polysomes was the population of free polysomes (FP)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under osmotic stress, the dominant population of polysomes was the population of free polysomes (FP)”
t0“pea”
Claim · model-written summary
Intensive osmotic stress increased the shares of MBP, CBP and CMBP.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- intensive (−1.0 and −1.5 MPa) osmotic stresse0
- Outcome
- share of membrane-bound polysomes (MBP) and cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The share of membrane-bound polysomes (MBP) and cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP) in the total fraction of ribosomes increased under intensive (−1.0 and −1.5 MPa) osmotic stress”
t0“pea”
Claim · model-written summary
Stress-formed polysomes, especially CMBP, resisted exogenous ribonucleases more than controls.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- osmotic stress conditionse0
- Outcome
- resistant to the activity of exogenous ribonucleasese0
- Direction
- more resistante0
- Qualifier
- especially the CMBPe0
- Comparator
- control samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polysomes formed under osmotic stress conditions (especially the CMBP) were more resistant to the activity of exogenous ribonucleases than the polysomes in the control samples”
t0“pea”
Claim · model-written summary
Bound polysomes may support stress protein synthesis.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- synthesis of stress proteinse0
- Qualifier
- bound forms of polysomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bound forms of polysomes play an important role in the synthesis of stress proteins”
t0“pea”
Claim · model-written summary
Dense ribosome packing on mRNA under stress may increase ribonuclease resistance.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- more resistant to ribonucleasee0
- Qualifier
- ribosomes become more densely packed on mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under stress conditions it is highly likely that ribosomes become more densely packed on mRNA thus making it more resistant to ribonuclease”
t0“pea”
The same questions in other papers
drought · disease · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.