Abstract
Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) is an emerging and serious pathogen affecting several Allium spp. worldwide (2). The virus causes straw-colored, chlorotic or necrotic lesions that coalesce, occasionally resulting in an extensive necrosis on onion (A. cepa L.) leaves. From February to June 2008, 530 onion and 439 leek (A. porum L.) leaf samples showing a variety of lesions were collected from different areas of Greece. All plants sampled were infested with Thrips tabaci Lindeman, the sole thrips species identified as the vector of this virus. Samples were analyzed by double-antibody sandwich (DAS)-ELISA using polyclonal antibodies against the N protein of IYSV (Laboratory of Virology, Wageningen Agricultural University). A higher percentage of onion samples than leek samples were infected, with IYSV detected in 36, 44, 23.7, 61.7, 10, 55, 15.3, and 9.4% of onion samples from the prefectures of Evros, Heraklion, Kavala, Magnissia, Pella, Rodopi, Thessaloniki, and Viotia, respectively, and in 5, 0, 0, 9.3, and 13% of leek samples from Evros, Heraklion, Magnissia, Pella, and Thessaloniki, respectively. No leek samples were tested from Kavala, Rodopi, and Viotia. Sap extracts from some positive samples were mechanically inoculated onto Nicotiana benthamiana leaves, and infected plants developed typical IYSV symptoms and were positive in DAS-ELISA, confirming transmission from the field samples. Viral RNA was extracted from ELISA-positive onion and leek samples and an ~800-bp amplicon was obtained from both hosts by reverse-transcription (RT)-PCR and N-gene primers derived from IYSV (IY1: 5'-CCCGAGGATCCATGGCTACCGTTAGGG-3' and IY2: 5'-CCCGAGGATCCAAATTAATTATATCTATCTTTCTTGG-3') (1). These amplicons were cloned and sequenced (GenBank Accession No. FJ785835) and nucleotide sequence comparisons showed a 98 to 99% identity with a Dutch isolate of IYSV (GenBank Accession No AF001387). The virus was transmitted among onion seedlings in the laboratory using a leek population of T. tabaci. Infected seedlings, as determined by DAS-ELISA, developed symptoms similar to those observed in the field samples. To our knowledge, this is the first report of IYSV in Greece; however, the virus seems already to be very well established. References: (1) I. Cortez et al. Phytopathology 88:1276, 1998. (2) D. Gent et al. Plant. Dis. 90:1468, 2006.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
All sampled onion and leek plants were infested with Thrips tabaci.
- Taxon
- Onion and Leek
Allium cepa
Allium ampeloprasumt0 - Interactor
- Thrips tabaci Lindeman
Thrips tabacie0pest - Outcome
- infested with Thrips tabaci Lindemane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All plants sampled were infested with Thrips tabaci Lindeman”
t0“Onion and Leek”
Claim · model-written summary
IYSV was detected in onion samples across eight Greek prefectures at the reported incidences.
- Taxon
- onionAllium cepae0
- Interactor
- IYSVe0pathogen
- Outcome
- IYSV detectede0
- Value
- 36, 44, 23.7, 61.7, 10, 55, 15.3, and 9.4%e0
- Setting
- Evros, Heraklion, Kavala, Magnissia, Pella, Rodopi, Thessaloniki, and Viotiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IYSV detected in 36, 44, 23.7, 61.7, 10, 55, 15.3, and 9.4% of onion samples from the prefectures of Evros, Heraklion, Kavala, Magnissia, Pella, Rodopi, Thessaloniki, and Viotia, respectively”
Claim · model-written summary
IYSV was detected in leek samples across five Greek prefectures at the reported incidences.
- Taxon
- leekAllium ampeloprasume0
- Outcome
- leek samplese0
- Value
- 5, 0, 0, 9.3, and 13%e0
- Setting
- Evros, Heraklion, Magnissia, Pella, and Thessalonikie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in 5, 0, 0, 9.3, and 13% of leek samples from Evros, Heraklion, Magnissia, Pella, and Thessaloniki, respectively”
Claim · model-written summary
A higher fraction of onion samples than leek samples were infected.
- Taxon
- onionAllium cepae0
- Outcome
- percentage of onion samplese0
- Direction
- highere0
- Comparator
- leek samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A higher percentage of onion samples than leek samples were infected”
Claim · model-written summary
Inoculated Nicotiana benthamiana developed typical IYSV symptoms and tested positive by ELISA.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- leavese0
- Treatment
- Sap extracts from some positive samples were mechanically inoculatede0
- Interactor
- IYSVe0pathogen
- Outcome
- typical IYSV symptoms and were positive in DAS-ELISAe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sap extracts from some positive samples were mechanically inoculated onto Nicotiana benthamiana leaves, and infected plants developed typical IYSV symptoms and were positive in DAS-ELISA”
Claim · model-written summary
The onion and leek viral amplicons showed 98 to 99% sequence identity to a Dutch IYSV isolate.
- Taxon
- Onion and LeekAllium cepaAllium ampeloprasumt0
- Interactor
- IYSVe0pathogen
- Outcome
- identity with a Dutch isolate of IYSVe0
- Value
- 98 to 99%e0
- Comparator
- Dutch isolate of IYSVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nucleotide sequence comparisons showed a 98 to 99% identity with a Dutch isolate of IYSV”
t0“Onion and Leek”
Claim · model-written summary
A leek population of T. tabaci transmitted the virus among onion seedlings.
- Taxon
- onionAllium cepae0
- Organ / stage
- seedlingse0
- Interactor
- T. tabaciThrips tabacie0pest
- Outcome
- virus was transmitted among onion seedlingse0
- Setting
- laboratorye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virus was transmitted among onion seedlings in the laboratory using a leek population of T. tabaci”
Claim · model-written summary
The infected onion seedlings developed symptoms resembling those in field samples.
- Taxon
- onionAllium cepat0
- Outcome
- developed symptoms similar to those observed in the field samplese0
- Comparator
- field samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infected seedlings, as determined by DAS-ELISA, developed symptoms similar to those observed in the field samples”
t0“Onion”
Claim · model-written summary
The authors reported IYSV in Greece for the first time.
- Taxon
- Onion and LeekAllium cepaAllium ampeloprasumt0
- Interactor
- IYSVe0pathogen
- Outcome
- first report of IYSVe0
- Setting
- Greecee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this is the first report of IYSV in Greece”
t0“Onion and Leek”
The same questions in other papers
botanical · disease · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
2 papers more in this group
See all 6 papers →inoculation · disease · Nicotiana benthamianaPositive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.