Paper · 1969 · PLANT PHYSIOLOGY

Gibberellin and CCC Effects on Flowering and Growth in the Long-day Plant Lemna gibba G3

Charles F. Cleland; Winslow S. Briggs

11
claims
20
verbatim quotes
44
citations
2
research areas
✓
Open access · cc-by

Abstract

The application of gibberellic acid (GA(3)) to the non-rosette long-day plant Lemna gibba G3 at concentrations from 0.1 to 100 mg/l did not induce flowering on short days and inhibited flowering on long days at concentrations of 1 mg/l and higher. On both short and long days GA(3) concentrations above 1 mg/l caused a decrease in frond size and fresh and dry weight, but an increase in the rate of frond production and thus an increase in the # VF (number of vegetative fronds). Identical results were obtained when gibberellin A(7) was used instead of GA(3).The addition of the plant growth retardant CCC [(2-chloroethyl) trimethylammonium chloride] to the culture medium on long days resulted in almost complete inhibition of flowering at 10(-3) M. Vegetative growth was also inhibited to some extent. With CCC at 10(-3) M the simultaneous addition of GA(3) resulted in partial reversal of flower inhibition with 0.3 mg/l GA(3) being optimal. The inhibition of vegetative growth as measured by fresh and dry weight was also partially reversed by GA(3), but the threshold concentration for reversal of flower inhibition was at least 10 times lower than that for inhibition of vegetative growth.These results are interpreted as indicating that gibberellins are important for flowering in the non-rosette long-day plant L. gibba G3, but apparently are present in non-limiting concentrations on short days.

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Claims 11

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Gibberellic acid at 0.1 to 100 mg/l did not induce flowering in Lemna gibba G3 on short days.

Taxon
Lemna gibba G3Lemna gibbae0
Treatment
gibberellic acid (GA(3))e0
Outcome
floweringe0
Direction
did not inducee0
Qualifier
on short dayse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The application of gibberellic acid (GA(3)) to the non-rosette long-day plant Lemna gibba G3 at concentrations from 0.1 to 100 mg/l did not induce flowering on short days”

✓ located verbatim in the source · abstract, characters 0–170 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Gibberellic acid inhibited flowering of Lemna gibba G3 on long days at concentrations of 1 mg/l and higher.

Taxon
Lemna gibba G3Lemna gibbae0
Treatment
gibberellic acid (GA(3))e0
Outcome
floweringe0
Direction
inhibitede0
Qualifier
at concentrations of 1 mg/l and highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The application of gibberellic acid (GA(3)) to the non-rosette long-day plant Lemna gibba G3 at concentrations from 0.1 to 100 mg/l did not induce flowering on short days and inhibited flowering on long days at concentrations of 1 mg/l and higher”

✓ located verbatim in the source · abstract, characters 0–246 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

On both short and long days GA3 above 1 mg/l decreased frond size and fresh and dry weight of Lemna gibba G3.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
GA(3) concentrations above 1 mg/le0
Outcome
frond size and fresh and dry weighte0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“On both short and long days GA(3) concentrations above 1 mg/l caused a decrease in frond size and fresh and dry weight”

✓ located verbatim in the source · abstract, characters 248–366 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

GA3 above 1 mg/l increased the rate of frond production and the number of vegetative fronds (# VF) in Lemna gibba G3.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
GA(3) concentrations above 1 mg/le0
Outcome
rate of frond productione0
Direction
increasee0
Qualifier
increase in the # VF (number of vegetative fronds)e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“GA(3) concentrations above 1 mg/l caused a decrease in frond size and fresh and dry weight, but an increase in the rate of frond production and thus an increase in the # VF (number of vegetative fronds)”

✓ located verbatim in the source · abstract, characters 276–478 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Gibberellin A7 gave results identical to GA3 in Lemna gibba G3.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
gibberellin A(7)e0
Outcome
Identical resultse0
Comparator
GA(3)e0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Identical results were obtained when gibberellin A(7) was used instead of GA(3)”

✓ located verbatim in the source · abstract, characters 480–559 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

CCC at 10(-3) M in the culture medium on long days almost completely inhibited flowering of Lemna gibba G3.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
addition of the plant growth retardant CCCe0
Outcome
floweringe0
Direction
almost complete inhibitione0
Qualifier
on long dayse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of the plant growth retardant CCC [(2-chloroethyl) trimethylammonium chloride] to the culture medium on long days resulted in almost complete inhibition of flowering at 10(-3) M”

✓ located verbatim in the source · abstract, characters 560–750 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

CCC also inhibited vegetative growth of Lemna gibba G3 to some extent.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
plant growth retardant CCCe0
Outcome
Vegetative growthe1
Direction
inhibitede1
Qualifier
to some extente1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The addition of the plant growth retardant CCC [(2-chloroethyl) trimethylammonium chloride] to the culture medium”

✓ located verbatim in the source · abstract, characters 560–673 · show in text ↑
e1

“Vegetative growth was also inhibited to some extent”

✓ located verbatim in the source · abstract, characters 752–803 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

With CCC at 10(-3) M, simultaneous addition of GA3 partially reversed the flower inhibition, with 0.3 mg/l GA3 optimal.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
simultaneous addition of GA(3)e0
Outcome
flower inhibitione0
Direction
partial reversale0
Qualifier
0.3 mg/l GA(3) being optimale0
Comparator
CCC at 10(-3) Me0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“With CCC at 10(-3) M the simultaneous addition of GA(3) resulted in partial reversal of flower inhibition with 0.3 mg/l GA(3) being optimal”

✓ located verbatim in the source · abstract, characters 805–944 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The CCC-induced inhibition of vegetative growth, measured by fresh and dry weight, was also partially reversed by GA3.

Taxon
Lemna gibba G3Lemna gibbat0
Treatment
GA(3)e0
Outcome
inhibition of vegetative growth as measured by fresh and dry weighte0
Direction
partially reversede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The inhibition of vegetative growth as measured by fresh and dry weight was also partially reversed by GA(3)”

✓ located verbatim in the source · abstract, characters 946–1054 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The threshold GA3 concentration for reversing flower inhibition was at least 10 times lower than that for reversing the inhibition of vegetative growth.

Taxon
Lemna gibba G3Lemna gibbat0
Outcome
threshold concentration for reversal of flower inhibitione0
Direction
lowere0
Value
at least 10 timese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the threshold concentration for reversal of flower inhibition was at least 10 times lower than that for inhibition of vegetative growth”

✓ located verbatim in the source · abstract, characters 1060–1195 · show in text ↑
t0

“Lemna gibba G3”

✓ located verbatim in the source · title, characters 74–88 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors interpret the results as showing that gibberellins are important for flowering in L. gibba G3 but are present in non-limiting concentrations on short days.

Taxon
L. gibba G3Lemna gibbae0
Compound
gibberellinse0
Outcome
floweringe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lemna gibba
Lemna gibba · Taxon · picture from its Wikimedia Commons categoryKarelj · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“gibberellins are important for flowering in the non-rosette long-day plant L. gibba G3, but apparently are present in non-limiting concentrations on short days”

✓ located verbatim in the source · abstract, characters 1245–1404 · show in text ↑