Abstract
The effects of osmotic dehydration on the photochemical activity, gene transcription, and protein content of photosystem 2 (PS 2) in leaves of two wheat (Triticum aestivum L.) cultivars Miannnong No. 4 and No. 5 were studied. Roots of both cultivars were submerged into polyethylene glycol (PEG) solutions with an osmotic potential of -0.5 MPa for 0, 24, 48, and 72 h. Relative water content (RWC) decreased markedly after 48 and 72 h. Simultaneously, marked increase in electrolyte leakage, decrease in contents of chlorophylls (Chl) a and b, and inhibition in PS 2 activity were observed. Northern hybridization indicated that progressive water stress remarkably reduced contents of the chloroplast gene psbA and psbD and nuclear gene cab transcripts. Urea-SDS-PAGE and Western blotting analysis showed that the contents of major PS 2 proteins, including the D1 and D2 proteins in the PS 2 reaction centre (RC) and the light-harvesting Chl a/b-protein complex (LHC 2) in periphery, declined with increasing water stress. Miannong No. 5 had less destroyed plasma membranes and higher RWC, Chl contents, and PS 2 activity during water stress than Miannong No. 4, which suggested its better drought resistance. The significant difference in steady state contents of LHC 2 proteins of two cultivars can be mainly attributed to the marked difference in transcript level of cab gene, which indicated that LHC 2 proteins protect PS 2 RC.
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Claims 10
Claim · model-written summary
Relative water content fell after 48 and 72 hours of PEG exposure.
- Taxon
- wheat
Triticum aestivumt0 - Outcome
- Relative water content (RWC)e0
- Direction
- decreasede0
- Qualifier
- after 48 and 72 he0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative water content (RWC) decreased markedly after 48 and 72 h.”
t0“wheat”
Claim · model-written summary
Electrolyte leakage increased under osmotic dehydration.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- electrolyte leakagee0
- Direction
- increasee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“marked increase in electrolyte leakage”
t0“wheat”
Claim · model-written summary
Chlorophyll a and b contents decreased under osmotic dehydration.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- contents of chlorophylls (Chl) a and be0
- Direction
- decreasee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decrease in contents of chlorophylls (Chl) a and b”
t0“wheat”
Claim · model-written summary
Photosystem 2 activity was inhibited under osmotic dehydration.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- PS 2 activitye0
- Direction
- inhibitione0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition in PS 2 activity”
t0“wheat”
Claim · model-written summary
Progressive water stress reduced psbA, psbD and cab transcripts.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- progressive water stresse0
- Gene
- psbA and psbD and nuclear gene cabe0
- Outcome
- contents of the chloroplast gene psbA and psbD and nuclear gene cab transcriptse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“progressive water stress remarkably reduced contents of the chloroplast gene psbA and psbD and nuclear gene cab transcripts”
t0“wheat”
Claim · model-written summary
Major PS 2 protein contents declined with increasing water stress.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- increasing water stresse0
- Gene
- D1 and D2 proteinse0
- Outcome
- contents of major PS 2 proteinse0
- Direction
- declinede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contents of major PS 2 proteins, including the D1 and D2 proteins in the PS 2 reaction centre (RC) and the light-harvesting Chl a/b-protein complex (LHC 2) in periphery, declined with increasing water stress”
t0“wheat”
Claim · model-written summary
Miannong No. 5 retained higher water content, chlorophyll content and PS 2 activity than Miannong No. 4 under stress.
- Taxon
- wheatTriticum aestivumt0
- Material
- Miannong No. 5e0
- Outcome
- RWC, Chl contents, and PS 2 activitye0
- Direction
- highere0
- Comparator
- Miannong No. 4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Miannong No. 5 had less destroyed plasma membranes and higher RWC, Chl contents, and PS 2 activity during water stress than Miannong No. 4”
t0“wheat”
Claim · model-written summary
Miannong No. 5 had less plasma membrane destruction than Miannong No. 4.
- Taxon
- wheatTriticum aestivumt0
- Material
- Miannong No. 5e0
- Outcome
- destroyed plasma membranese0
- Direction
- lesse0
- Comparator
- Miannong No. 4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Miannong No. 5 had less destroyed plasma membranes and higher RWC, Chl contents, and PS 2 activity during water stress than Miannong No. 4”
t0“wheat”
Claim · model-written summary
The authors attribute cultivar differences in LHC 2 proteins to cab transcript levels.
- Taxon
- wheatTriticum aestivumt0
- Gene
- cab genee0
- Outcome
- steady state contents of LHC 2 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“difference in steady state contents of LHC 2 proteins of two cultivars can be mainly attributed to the marked difference in transcript level of cab gene”
t0“wheat”
Claim · model-written summary
The authors infer that LHC 2 proteins protect the PS 2 reaction centre.
- Taxon
- wheatTriticum aestivumt0
- Gene
- LHC 2 proteinse0
- Outcome
- PS 2 RCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LHC 2 proteins protect PS 2 RC”
t0“wheat”
The same questions in other papers
drought · molecular: expression · Triticum aestivum (wheat)Positive and negative results
23 papers in the same direction as this paper · 74 in the opposite direction
- positive
- positive
- positive
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See all 98 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.