Abstract
A full-length cDNA and the corresponding At-P5S gene encoding the first enzyme of the proline biosynthetic pathway, the delta 1-pyrroline-5-carboxylate (P5C) synthetase, were isolated in Arabidopsis thaliana. The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia. Strong homology is also found at the N-terminus to bacterial and yeast gamma-glutamyl kinase and at the C-terminus to bacterial gamma-glutamyl phosphate reductase. Putative ATP- and NAD(P)H-binding sites are suggested in the At-P5S protein. The transcribed region of the At-P5S gene is 4.8 kb long and contains 20 exons. Southern analysis suggests the presence of only one At-P5S gene in the A. thaliana genome mapped at the bottom of the chromosome two. Expression analysis of At-P5S in different organs reveals abundant At-P5S transcripts in mature flowering plant. Rapid induction of the At-P5S gene followed by accumulation of proline was observed in NaCl-treated seedlings suggesting that At-P5S is osmoregulated.
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Claims 11
Claim · model-written summary
The At-P5S cDNA and gene encoding P5C synthetase were isolated.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- At-P5Se0
- Outcome
- were isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A full-length cDNA and the corresponding At-P5S gene encoding the first enzyme of the proline biosynthetic pathway, the delta 1-pyrroline-5-carboxylate (P5C) synthetase, were isolated in Arabidopsis thaliana.”
Claim · model-written summary
At-P5S cDNA encodes a 717-amino-acid protein highly similar to Vigna aconitifolia P5C synthetase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- At-P5Se0
- Outcome
- high identitye0
- Value
- 717 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia.”
t0“Arabidopsis thaliana”
Claim · model-written summary
At-P5S has N-terminal homology to bacterial and yeast gamma-glutamyl kinase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- At-P5Se0
- Outcome
- Strong homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong homology is also found at the N-terminus to bacterial and yeast gamma-glutamyl kinase and at the C-terminus to bacterial gamma-glutamyl phosphate reductase. Putative ATP- and NAD(P)H-binding sites are suggested in the At-P5S protein.”
t0“Arabidopsis thaliana”
Claim · model-written summary
At-P5S has C-terminal homology to bacterial gamma-glutamyl phosphate reductase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- bacterial gamma-glutamyl phosphate reductasee0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the C-terminus to bacterial gamma-glutamyl phosphate reductase”
t0“Arabidopsis thaliana”
Claim · model-written summary
Putative ATP and NAD(P)H binding sites were suggested in At-P5S.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- At-P5Se0
- Outcome
- ATP- and NAD(P)H-binding sitese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Putative ATP- and NAD(P)H-binding sites are suggested in the At-P5S protein.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The transcribed At-P5S region is 4.8 kb and has 20 exons.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- At-P5Se0
- Outcome
- transcribed regione0
- Value
- 4.8 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcribed region of the At-P5S gene is 4.8 kb long and contains 20 exons.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Southern analysis suggested one At-P5S gene at the bottom of chromosome two.
- Taxon
- A. thalianaArabidopsis thalianae0
- Gene
- At-P5Se0
- Outcome
- only one At-P5S genee0
- Setting
- bottom of the chromosome twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Southern analysis suggests the presence of only one At-P5S gene in the A. thaliana genome mapped at the bottom of the chromosome two.”
Claim · model-written summary
At-P5S transcripts were abundant in mature flowering plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- mature flowering plante0
- Gene
- At-P5Se0
- Outcome
- abundant At-P5S transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression analysis of At-P5S in different organs reveals abundant At-P5S transcripts in mature flowering plant.”
t0“Arabidopsis thaliana”
Claim · model-written summary
NaCl treatment rapidly induced At-P5S expression in seedlings.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- seedlingse0
- Treatment
- NaCl-treatede0
- Gene
- At-P5Se0
- Outcome
- At-P5S genee0
- Direction
- Rapid inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rapid induction of the At-P5S gene followed by accumulation of proline was observed in NaCl-treated seedlings”
t0“Arabidopsis thaliana”
Claim · model-written summary
NaCl treatment was followed by proline accumulation in seedlings.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- seedlingse0
- Treatment
- NaCl-treatede0
- Outcome
- accumulation of prolinee0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rapid induction of the At-P5S gene followed by accumulation of proline was observed in NaCl-treated seedlings”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors interpret At-P5S as osmoregulated.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- At-P5Se0
- Outcome
- osmoregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that At-P5S is osmoregulated.”
t0“Arabidopsis thaliana”
The same questions in other papers
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7 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.