Abstract
In Arabidopsis, resistance to Turnip Crinkle Virus (TCV) depends on the resistance (R) gene, HRT, and the recessive locus rrt. Resistance also depends on salicylic acid (SA), EDS1, and PAD4. Exogenous application of SA confers resistance in RRT-containing plants by increasing HRT transcript levels in a PAD4-dependent manner. Here we report that reduction of oleic acid (18:1) can also induce HRT gene expression and confer resistance to TCV. However, the 18:1-regulated pathway is independent of SA, rrt, EDS1, and PAD4. Reducing the levels of 18:1, via a mutation in the SSI2-encoded stearoyl-acyl carrier protein-desaturase, or by exogenous application of glycerol, increased transcript levels of HRT as well as several other R genes. Second-site mutations in the ACT1-encoded glycerol-3-phosphate acyltransferase or GLY1-encoded glycerol-3-phosphate dehydrogenase restored 18:1 levels in HRT ssi2 plants and reestablished a dependence on rrt. Resistance to TCV and HRT gene expression in HRT act1 plants was inducible by SA but not by glycerol, whereas that in HRT pad4 plants was inducible by glycerol but not by SA. The low 18:1-mediated induction of R gene expression was also dependent on ACT1 but independent of EDS1, PAD4, and RAR1. Intriguingly, TCV inoculation did not activate this 18:1-regulated pathway in HRT plants, but instead resulted in the induction of several genes that encode 18:1-synthesizing isozymes. These results suggest that the 18:1-regulated pathway may be specifically targeted during pathogen infection and that altering 18:1 levels may serve as a unique strategy for promoting disease resistance.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Reduction of oleic acid (18:1) induced HRT gene expression in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- reduction of oleic acid (18:1)e1
- Gene
- HRTe1
- Compound
- oleic acid (18:1)e1
- Outcome
- HRT gene expressione1
- Direction
- inducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e1“Here we report that reduction of oleic acid (18:1) can also induce HRT gene expression and confer resistance to TCV”
Claim · model-written summary
Reduction of oleic acid (18:1) conferred resistance to TCV in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- reduction of oleic acid (18:1)e1
- Interactor
- TCVe1pathogen
- Outcome
- resistance to TCVe1
- Direction
- confere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e1“Here we report that reduction of oleic acid (18:1) can also induce HRT gene expression and confer resistance to TCV”
Claim · model-written summary
A mutation in the SSI2-encoded desaturase, which lowers 18:1, increased HRT and several other R gene transcript levels in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- a mutation in the SSI2-encoded stearoyl-acyl carrier protein-desaturasee2
- Gene
- HRTe2
- Outcome
- transcript levels of HRT as well as several other R genese2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e2“Reducing the levels of 18:1, via a mutation in the SSI2-encoded stearoyl-acyl carrier protein-desaturase, or by exogenous application of glycerol, increased transcript levels of HRT as well as several other R genes”
Claim · model-written summary
Exogenous glycerol, which lowers 18:1, increased HRT and several other R gene transcript levels in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- exogenous application of glycerole2
- Gene
- HRTe2
- Outcome
- transcript levels of HRT as well as several other R genese2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e2“Reducing the levels of 18:1, via a mutation in the SSI2-encoded stearoyl-acyl carrier protein-desaturase, or by exogenous application of glycerol, increased transcript levels of HRT as well as several other R genes”
Claim · model-written summary
Second-site mutations in ACT1 or GLY1 restored 18:1 levels in HRT ssi2 Arabidopsis plants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- HRT ssi2 plantse3
- Treatment
- Second-site mutations in the ACT1-encoded glycerol-3-phosphate acyltransferase or GLY1-encoded glycerol-3-phosphate dehydrogenasee3
- Gene
- ACT1e3
- Outcome
- 18:1 levelse3
- Direction
- restorede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e3“Second-site mutations in the ACT1-encoded glycerol-3-phosphate acyltransferase or GLY1-encoded glycerol-3-phosphate dehydrogenase restored 18:1 levels in HRT ssi2 plants and reestablished a dependence on rrt”
Claim · model-written summary
Second-site mutations in ACT1 or GLY1 reestablished a dependence on rrt in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Second-site mutations in the ACT1-encoded glycerol-3-phosphate acyltransferase or GLY1-encoded glycerol-3-phosphate dehydrogenasee3
- Gene
- rrte3
- Outcome
- dependence on rrte3
- Direction
- reestablishede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e3“Second-site mutations in the ACT1-encoded glycerol-3-phosphate acyltransferase or GLY1-encoded glycerol-3-phosphate dehydrogenase restored 18:1 levels in HRT ssi2 plants and reestablished a dependence on rrt”
Claim · model-written summary
The 18:1-regulated pathway in Arabidopsis is independent of SA, rrt, EDS1, and PAD4.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- 18:1-regulated pathwaye5
- Qualifier
- independent of SA, rrt, EDS1, and PAD4e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e5“However, the 18:1-regulated pathway is independent of SA, rrt, EDS1, and PAD4”
Claim · model-written summary
The low 18:1-mediated induction of R gene expression in Arabidopsis depends on ACT1 but not on EDS1, PAD4 or RAR1.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ACT1e4
- Outcome
- induction of R gene expressione4
- Qualifier
- dependent on ACT1 but independent of EDS1, PAD4, and RAR1e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e4“The low 18:1-mediated induction of R gene expression was also dependent on ACT1 but independent of EDS1, PAD4, and RAR1”
Claim · model-written summary
Resistance to TCV and HRT gene expression in HRT act1 Arabidopsis plants was inducible by SA but not by glycerol.
- Taxon
- ArabidopsisArabidopsise0
- Material
- HRT act1 plantse6
- Treatment
- SAe6
- Outcome
- Resistance to TCV and HRT gene expressione6
- Direction
- induciblee6
- Qualifier
- but not by glycerole6
- Comparator
- glycerole6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e6“Resistance to TCV and HRT gene expression in HRT act1 plants was inducible by SA but not by glycerol, whereas that in HRT pad4 plants was inducible by glycerol but not by SA”
Claim · model-written summary
Resistance to TCV and HRT gene expression in HRT pad4 Arabidopsis plants was inducible by glycerol but not by SA.
- Taxon
- ArabidopsisArabidopsise0
- Material
- HRT pad4 plantse6
- Treatment
- glycerole6
- Outcome
- Resistance to TCV and HRT gene expressione6
- Direction
- induciblee6
- Qualifier
- but not by SAe6
- Comparator
- SAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e6“Resistance to TCV and HRT gene expression in HRT act1 plants was inducible by SA but not by glycerol, whereas that in HRT pad4 plants was inducible by glycerol but not by SA”
Claim · model-written summary
TCV inoculation did not activate the 18:1-regulated pathway in HRT Arabidopsis plants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- HRT plantse7
- Treatment
- TCV inoculatione7
- Outcome
- 18:1-regulated pathwaye7
- Direction
- did not activatee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e7“Intriguingly, TCV inoculation did not activate this 18:1-regulated pathway in HRT plants, but instead resulted in the induction of several genes that encode 18:1-synthesizing isozymes”
Claim · model-written summary
TCV inoculation induced several genes that encode 18:1-synthesizing isozymes in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- TCV inoculatione7
- Gene
- genes that encode 18:1-synthesizing isozymese7
- Outcome
- induction of several genes that encode 18:1-synthesizing isozymese7
- Direction
- inductione7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e7“Intriguingly, TCV inoculation did not activate this 18:1-regulated pathway in HRT plants, but instead resulted in the induction of several genes that encode 18:1-synthesizing isozymes”
Claim · model-written summary
The 18:1-regulated pathway may be specifically targeted during pathogen infection in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- 18:1-regulated pathwaye1
- Qualifier
- may be specifically targeted during pathogen infectione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e1“These results suggest that the 18:1-regulated pathway may be specifically targeted during pathogen infection”
Claim · model-written summary
Altering 18:1 levels may serve as a strategy for promoting disease resistance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- altering 18:1 levelse1
- Qualifier
- a unique strategy for promoting disease resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, resistance to Turnip Crinkle Virus (TCV)”
e1“altering 18:1 levels may serve as a unique strategy for promoting disease resistance”
The same questions in other papers
salicylic acid · molecular: expression · ArabidopsisPositive and negative results
25 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
- no effect
37 papers more in this group
See all 41 papers →loss of function · molecular: expression · ArabidopsisPositive and negative results
37 papers in the same direction as this paper · 159 in the opposite direction
- positive
- negative
- negative
193 papers more in this group
See all 197 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.