Abstract
Germinlike proteins (GLP) are encoded in plants by a gene family with proposed functions in plant development and defense. Genes of GLP subfamily 4 of barley (HvGLP4, formerly referred to as HvOxOLP) and the wheat orthologue TaGLP4 (formerly referred to as TaGLP2a) were previously found to be expressed in pathogen-attacked epidermal tissue of barley and wheat leaves, and the corresponding proteins are proposed to accumulate in the apoplast. Here, the role of HvGLP4 and TaGLP4 in the defense of barley and wheat against Blumeria graminis (DC.) E. O. Speer, the cereal powdery mildew fungus, was examined in an epidermal transient expression system and in transgenic Arabidopsis thaliana plants overexpressing His-tagged HvGLP4. Leaf extracts of transgenic Arabidopsis overexpressing HvGLP4 contained a novel His-tagged protein with superoxide dismutase activity and HvGLP4 epitopes. Transient overexpression of TaGLP4 and HvGLP4 enhanced resistance against B. graminis in wheat and barley, whereas transient silencing by RNA interference reduced basal resistance in both cereals. The effect of GLP4 overexpression or silencing was strongly influenced by the genotype of the plant. The data suggest that members of GLP subfamily 4 are components of quantitative resistance in both barley and wheat, acting together with other, as yet unknown, plant components.
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Claims 7
Claim · model-written summary
Arabidopsis overexpressing HvGLP4 contained a His-tagged protein with superoxide dismutase activity.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- HvGLP4e0
- Outcome
- superoxide dismutase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf extracts of transgenic Arabidopsis overexpressing HvGLP4 contained a novel His-tagged protein with superoxide dismutase activity and HvGLP4 epitopes.”
Claim · model-written summary
TaGLP4 overexpression enhanced wheat resistance to B. graminis.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- Transient overexpression of TaGLP4e0
- Interactor
- B. graminis
Blumeria graminise0pathogen - Gene
- TaGLP4e0
- Outcome
- resistance against B. graminise0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of TaGLP4 and HvGLP4 enhanced resistance against B. graminis in wheat and barley”
Claim · model-written summary
HvGLP4 overexpression enhanced barley resistance to B. graminis.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- overexpression of TaGLP4 and HvGLP4e0
- Interactor
- B. graminisBlumeria graminise0pathogen
- Gene
- HvGLP4e0
- Outcome
- resistance against B. graminise0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Transient overexpression of TaGLP4 and HvGLP4 enhanced resistance against B. graminis in wheat and barley”
Claim · model-written summary
RNA interference silencing reduced wheat basal resistance.
- Taxon
- wheatTriticum aestivume1
- Treatment
- transient silencing by RNA interferencee0
- Outcome
- basal resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing by RNA interference reduced basal resistance in both cereals”
e1“wheat and barley”
Claim · model-written summary
RNA interference silencing reduced barley basal resistance.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- transient silencing by RNA interferencee0
- Outcome
- basal resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing by RNA interference reduced basal resistance in both cereals”
e1“wheat and barley”
Claim · model-written summary
Genotype strongly influenced the resistance effect of GLP4 manipulation.
Not reportedDirection
Verbatim
e0“The effect of GLP4 overexpression or silencing was strongly influenced by the genotype of the plant.”
Claim · model-written summary
GLP subfamily 4 appears to contribute to quantitative resistance in barley and wheat.
- Taxon
- barley and wheatHordeum vulgareTriticum aestivume0
- Gene
- GLP subfamily 4e0
- Outcome
- quantitative resistancee0
- Qualifier
- acting together with other, as yet unknown, plant componentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“members of GLP subfamily 4 are components of quantitative resistance in both barley and wheat, acting together with other, as yet unknown, plant components”
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