Abstract
Although phosphate (Pi) starvation signaling is well studied in Arabidopsis (Arabidopsis thaliana), it is still largely unknown in rice (Oryza sativa). In this work, a rice leaf tip necrosis1 (ltn1) mutant was identified and characterized. Map-based cloning identified LTN1 as LOC_Os05g48390, the putative ortholog of Arabidopsis PHO2, which plays important roles in Pi starvation signaling. Analysis of transgenic plants harboring a LTN1 promoter::β-glucuronidase construct revealed that LTN1 was preferentially expressed in vascular tissues. The ltn1 mutant exhibited increased Pi uptake and translocation, which led to Pi overaccumulation in shoots. In association with enhanced Pi uptake and transport, some Pi transporters were up-regulated in the ltn1 mutant in the presence of sufficient Pi. Furthermore, the elongation of primary and adventitious roots was enhanced in the ltn1 mutant under Pi starvation, suggesting that LTN1 is involved in Pi-dependent root architecture alteration. Under Pi-sufficient conditions, typical Pi starvation responses such as stimulation of phosphatase and RNase activities, lipid composition alteration, nitrogen assimilation repression, and increased metal uptake were also activated in ltn1. Moreover, analysis of OsmiR399-overexpressing plants showed that LTN1 was down-regulated by OsmiR399. Our results strongly indicate that LTN1 is a crucial Pi starvation signaling component downstream of miR399 involved in the regulation of multiple Pi starvation responses in rice.
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Claims 14
Claim · model-written summary
A rice ltn1 mutant was identified and characterized.
- Taxon
- rice
Oryza sativae0 - Material
- leaf tip necrosis1 (ltn1) mutante0
- Outcome
- identified and characterizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a rice leaf tip necrosis1 (ltn1) mutant was identified and characterized.”
Claim · model-written summary
LTN1 was mapped to LOC_Os05g48390, a putative PHO2 ortholog.
- Taxon
- RiceOryza sativat0
- Gene
- LTN1e0
- Outcome
- LTN1 as LOC_Os05g48390e0
- Qualifier
- the putative ortholog of Arabidopsis PHO2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Map-based cloning identified LTN1 as LOC_Os05g48390, the putative ortholog of Arabidopsis PHO2”
t0“Rice”
Claim · model-written summary
LTN1 was preferentially expressed in rice vascular tissues.
- Taxon
- RiceOryza sativat0
- Organ / stage
- vascular tissuese0
- Gene
- LTN1e0
- Outcome
- preferentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LTN1 was preferentially expressed in vascular tissues.”
t0“Rice”
Claim · model-written summary
The rice ltn1 mutant had increased Pi uptake and translocation.
- Taxon
- RiceOryza sativat0
- Material
- ltn1 mutante0
- Treatment
- ltn1 mutante0
- Outcome
- Pi uptake and translocatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ltn1 mutant exhibited increased Pi uptake and translocation”
t0“Rice”
Claim · model-written summary
Increased uptake and translocation led to Pi overaccumulation in rice mutant shoots.
- Taxon
- RiceOryza sativat0
- Material
- ltn1 mutante0
- Organ / stage
- shootse0
- Outcome
- Pi overaccumulatione0
- Qualifier
- increased Pi uptake and translocatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ltn1 mutant exhibited increased Pi uptake and translocation, which led to Pi overaccumulation in shoots.”
t0“Rice”
Claim · model-written summary
Some Pi transporters were upregulated in rice ltn1 under sufficient Pi.
- Taxon
- RiceOryza sativat0
- Material
- ltn1 mutante0
- Treatment
- ltn1 mutante0
- Gene
- Pi transporterse0
- Outcome
- Pi transporterse0
- Direction
- up-regulatede0
- Qualifier
- in the presence of sufficient Pie0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some Pi transporters were up-regulated in the ltn1 mutant in the presence of sufficient Pi.”
t0“Rice”
Claim · model-written summary
Primary and adventitious root elongation was enhanced in rice ltn1 during Pi starvation.
- Taxon
- RiceOryza sativat0
- Organ / stage
- primary and adventitious rootse0
- Treatment
- ltn1 mutante0
- Outcome
- elongation of primary and adventitious rootse0
- Direction
- enhancede0
- Qualifier
- under Pi starvatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the elongation of primary and adventitious roots was enhanced in the ltn1 mutant under Pi starvation”
t0“Rice”
Claim · model-written summary
LTN1 is inferred to participate in Pi-dependent rice root architecture changes.
- Taxon
- RiceOryza sativat0
- Gene
- LTN1e0
- Outcome
- Pi-dependent root architecture alteratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that LTN1 is involved in Pi-dependent root architecture alteration.”
t0“Rice”
Claim · model-written summary
Under sufficient Pi, rice ltn1 activated phosphatase and RNase stimulation and lipid composition alteration.
- Taxon
- RiceOryza sativat0
- Treatment
- ltn1e0
- Outcome
- stimulation of phosphatase and RNase activitiese0
- Direction
- stimulatione0
- Qualifier
- Under Pi-sufficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under Pi-sufficient conditions, typical Pi starvation responses such as stimulation of phosphatase and RNase activities, lipid composition alteration, nitrogen assimilation repression, and increased metal uptake were also activated in ltn1.”
t0“Rice”
Claim · model-written summary
Rice ltn1 showed lipid composition alteration under sufficient Pi.
- Taxon
- RiceOryza sativat0
- Treatment
- ltn1e0
- Outcome
- lipid composition alteratione0
- Qualifier
- Under Pi-sufficient conditionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under Pi-sufficient conditions, typical Pi starvation responses such as stimulation of phosphatase and RNase activities, lipid composition alteration, nitrogen assimilation repression, and increased metal uptake were also activated in ltn1.”
t0“Rice”
Claim · model-written summary
Rice ltn1 showed nitrogen assimilation repression under sufficient Pi.
- Taxon
- RiceOryza sativat0
- Treatment
- ltn1e0
- Outcome
- nitrogen assimilatione0
- Direction
- repressione0
- Qualifier
- Under Pi-sufficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under Pi-sufficient conditions, typical Pi starvation responses such as stimulation of phosphatase and RNase activities, lipid composition alteration, nitrogen assimilation repression, and increased metal uptake were also activated in ltn1.”
t0“Rice”
Claim · model-written summary
Rice ltn1 increased metal uptake under sufficient Pi.
- Taxon
- RiceOryza sativat0
- Treatment
- ltn1e0
- Outcome
- metal uptakee0
- Direction
- increasede0
- Qualifier
- Under Pi-sufficient conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under Pi-sufficient conditions, typical Pi starvation responses such as stimulation of phosphatase and RNase activities, lipid composition alteration, nitrogen assimilation repression, and increased metal uptake were also activated in ltn1.”
t0“Rice”
Claim · model-written summary
OsmiR399 downregulated LTN1 in rice overexpression plants.
- Taxon
- RiceOryza sativat0
- Treatment
- OsmiR399-overexpressinge0
- Gene
- LTN1e0
- Outcome
- LTN1e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of OsmiR399-overexpressing plants showed that LTN1 was down-regulated by OsmiR399.”
t0“Rice”
Claim · model-written summary
LTN1 is interpreted as a crucial component downstream of miR399 regulating multiple rice Pi starvation responses.
- Taxon
- riceOryza sativae0
- Gene
- LTN1e0
- Outcome
- regulation of multiple Pi starvation responsese0
- Qualifier
- downstream of miR399e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LTN1 is a crucial Pi starvation signaling component downstream of miR399 involved in the regulation of multiple Pi starvation responses in rice.”
The same questions in other papers
loss of function · growth · Oryza sativa (rice)Positive and negative results
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