Abstract
To investigate gibberellin (GA) biosynthesis in mature tissue of pea ( Pisum sativum L.) in the absence of potentially GA-producing meristematic tissue we grafted wild-type scions to rootstocks of the GA-deficient ls - 1 mutant and later decapitated the shoot. After 2 d, decapitated shoots contained as much GA19 (a precursor of the bioactive GA1) as comparable tissue from intact plants, even though applied [14C]GA19 was metabolised rapidly during this time. These results show that the pool size of endogenous GA19 was maintained, probably by de novo GA19 synthesis. We also found that the LS gene, which catalyses an early step in GA biosynthesis, is expressed in mature tissue, as are the shoot-expressed GA 20-oxidase and GA 3-oxidase genes. Nevertheless, mature tissue contained very low levels of GA1 and GA20 compared with immature tissue. Levels of GA19, GA29 and GA8 were less affected by tissue age. Metabolism studies using 14C-labelled GAs indicated that mature tissue rapidly converted GA19 to GA20 and GA20 to GA1; the latter step was promoted by IAA. However, the 2-oxidation steps GA1 to GA8, GA20 to GA29 and GA29 to GA29-catabolite appear to proceed very rapidly in mature tissue (regardless of IAA content), and we suggest this is the reason why GA1 and GA20 do not accumulate. This is supported by the high level of expression of a key GA 2-oxidase gene in mature tissue.
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Claims 11
Claim · model-written summary
Decapitated shoots contained as much GA19 as intact plants.
- Taxon
- pea ( Pisum sativum L.)
Lathyrus oleraceuse0 - Treatment
- decapitatede1
- Outcome
- GA19e1
- Direction
- no_changeinferrede1
- Comparator
- intact plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e1“After 2 d, decapitated shoots contained as much GA19 (a precursor of the bioactive GA1) as comparable tissue from intact plants”
Claim · model-written summary
The pool size of endogenous GA19 in mature pea tissue was probably maintained by de novo GA19 synthesis.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Outcome
- pool size of endogenous GA19e2
- Qualifier
- de novo GA19 synthesise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e2“These results show that the pool size of endogenous GA19 was maintained, probably by de novo GA19 synthesis”
Claim · model-written summary
The LS gene is expressed in mature pea tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Gene
- LS genee3
- Outcome
- expressed in mature tissuee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e3“We also found that the LS gene, which catalyses an early step in GA biosynthesis, is expressed in mature tissue, as are the shoot-expressed GA 20-oxidase and GA 3-oxidase genes”
Claim · model-written summary
The shoot-expressed GA 20-oxidase and GA 3-oxidase genes are expressed in mature pea tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Gene
- GA 20-oxidase and GA 3-oxidasee3
- Outcome
- shoot-expressede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e3“We also found that the LS gene, which catalyses an early step in GA biosynthesis, is expressed in mature tissue, as are the shoot-expressed GA 20-oxidase and GA 3-oxidase genes”
Claim · model-written summary
Mature pea tissue contained very low levels of GA1 and GA20 compared with immature tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Organ / stage
- mature tissuee4
- Outcome
- levels of GA1 and GA20e4
- Direction
- very lowe4
- Comparator
- immature tissuee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e4“Nevertheless, mature tissue contained very low levels of GA1 and GA20 compared with immature tissue”
Claim · model-written summary
Levels of GA19, GA29 and GA8 were less affected by tissue age.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Organ / stage
- tissue agee5
- Outcome
- Levels of GA19, GA29 and GA8e5
- Direction
- less affectede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e5“Levels of GA19, GA29 and GA8 were less affected by tissue age”
Claim · model-written summary
IAA promoted the conversion of GA20 to GA1 in mature pea tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Treatment
- IAAe6
- Outcome
- GA20 to GA1e6
- Direction
- promotede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e6“Metabolism studies using 14C-labelled GAs indicated that mature tissue rapidly converted GA19 to GA20 and GA20 to GA1; the latter step was promoted by IAA”
Claim · model-written summary
Mature pea tissue rapidly converted GA19 to GA20 and GA20 to GA1.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Organ / stage
- mature tissuee6
- Outcome
- GA19 to GA20 and GA20 to GA1e6
- Qualifier
- rapidly convertede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e6“Metabolism studies using 14C-labelled GAs indicated that mature tissue rapidly converted GA19 to GA20 and GA20 to GA1; the latter step was promoted by IAA”
Claim · model-written summary
The 2-oxidation steps appear to proceed very rapidly in mature pea tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Organ / stage
- mature tissuee7
- Outcome
- 2-oxidation steps GA1 to GA8, GA20 to GA29 and GA29 to GA29-catabolitee7
- Qualifier
- appear to proceed very rapidlye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e7“the 2-oxidation steps GA1 to GA8, GA20 to GA29 and GA29 to GA29-catabolite appear to proceed very rapidly in mature tissue (regardless of IAA content), and we suggest this is the reason why GA1 and GA20 do not accumulate”
Claim · model-written summary
Rapid 2-oxidation is suggested to be the reason why GA1 and GA20 do not accumulate in mature pea tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Outcome
- GA1 and GA20 do not accumulatee7
- Qualifier
- 2-oxidation stepse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e7“the 2-oxidation steps GA1 to GA8, GA20 to GA29 and GA29 to GA29-catabolite appear to proceed very rapidly in mature tissue (regardless of IAA content), and we suggest this is the reason why GA1 and GA20 do not accumulate”
Claim · model-written summary
A key GA 2-oxidase gene shows a high level of expression in mature pea tissue.
- Taxon
- pea ( Pisum sativum L.)Lathyrus oleraceuse0
- Organ / stage
- mature tissuee7
- Gene
- GA 2-oxidasee7
- Outcome
- high level of expressione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature tissue of pea ( Pisum sativum L.)”
e7“This is supported by the high level of expression of a key GA 2-oxidase gene in mature tissue”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.