Abstract
A recently described actinomycete species (Streptomyces araujoniae ASBV-1(T)) is effective against many phytopathogenic fungi. In this study, we evaluated the capacity of this species to inhibit Botrytis cinerea development in strawberry pseudofruit, and we identified the chemical structures of its bioactive compounds. An ethyl acetate crude extract (0.1 mg ml(-1)) of ASBV-1(T) fermentation broth completely inhibited fungus growth in strawberry pseudofruit under storage conditions. The crude extract was fractionated by preparative high-performance liquid chromatography; the active fraction was further evaluated by tandem mass spectrometry. ASBV-1(T) produced a multiantibiotic complex with ionophoric properties. This complex contained members of the macrotetralides class (including monactin, dinactin, trinactin, and tetranactin) and the cyclodepsipeptide valinomycin, all of which were active against B. cinerea. Furthermore, the addition of 2 mM MgSO4 and 1 mM ZnSO4 enhanced macrotetralide and valinomycin production, respectively, in the culture broth. These compounds are considered to be the main active molecules that S. araujoniae produces to control B. cinerea. Their low to moderate toxicity to humans and the environment justifies the application of ASBV-1(T) in biological control programs that aim to mitigate the damage caused by this phytopathogen.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Streptomyces extract completely inhibited Botrytis growth on stored strawberry pseudofruit.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Treatment
- ethyl acetate crude extract (0.1 mg ml(-1)) of ASBV-1(T) fermentation brothe0
- Interactor
- Botrytis cinerea
Botrytis cinereae1pathogen - Second interactor
- ASBV-1(T)e0biocontrol_agent
- Outcome
- fungus growthe0
- Direction
- completely inhibitede0
- Setting
- storage conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“An ethyl acetate crude extract (0.1 mg ml(-1)) of ASBV-1(T) fermentation broth completely inhibited fungus growth in strawberry pseudofruit under storage conditions.”
e1“we evaluated the capacity of this species to inhibit Botrytis cinerea development in strawberry pseudofruit”
Claim · model-written summary
ASBV-1(T) produced an ionophoric multiantibiotic complex containing macrotetralides and valinomycin active against B. cinerea.
- Taxon
- strawberryFragaria ×ananassae1
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Second interactor
- ASBV-1(T)e0biocontrol_agent
- Outcome
- multiantibiotic complex with ionophoric propertiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ASBV-1(T) produced a multiantibiotic complex with ionophoric properties. This complex contained members of the macrotetralides class (including monactin, dinactin, trinactin, and tetranactin) and the cyclodepsipeptide valinomycin, all of which were active against B. cinerea.”
e1“fungus growth in strawberry pseudofruit under storage conditions”
Claim · model-written summary
MgSO4 addition enhanced macrotetralide production in culture broth.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- 2 mM MgSO4e0
- Outcome
- macrotetralidee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of 2 mM MgSO4 and 1 mM ZnSO4 enhanced macrotetralide and valinomycin production, respectively, in the culture broth.”
e1“fungus growth in strawberry pseudofruit under storage conditions”
Claim · model-written summary
ZnSO4 addition enhanced valinomycin production in culture broth.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- 1 mM ZnSO4e0
- Outcome
- valinomycin productione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of 2 mM MgSO4 and 1 mM ZnSO4 enhanced macrotetralide and valinomycin production, respectively, in the culture broth.”
e1“fungus growth in strawberry pseudofruit under storage conditions”
Claim · model-written summary
The authors support applying ASBV-1(T) in biological control programs against B. cinerea.
- Taxon
- strawberryFragaria ×ananassae2
- Interactor
- B. cinereaBotrytis cinereae1pathogen
- Second interactor
- ASBV-1(T)e0biocontrol_agent
- Outcome
- application of ASBV-1(T) in biological control programse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their low to moderate toxicity to humans and the environment justifies the application of ASBV-1(T) in biological control programs that aim to mitigate the damage caused by this phytopathogen.”
e1“all of which were active against B. cinerea.”
e2“fungus growth in strawberry pseudofruit under storage conditions”
The same questions in other papers
zinc sulfate · yield · Fragaria x ananassa (strawberry)Results agree
13 papers in the same direction as this paper
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →processing · Botrytis cinerea · Fragaria x ananassa (strawberry)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →botanical · Botrytis cinerea · Fragaria x ananassa (strawberry)Positive and negative results
31 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.