Abstract
Cell wall glycanhydrolytic enzymes have been related to cell wall loosening and cell growth, although the mechanism of this relationship has not been clarified. Since auxins are plant hormones that stimulate growth in elongating organs, in the present work we studied the effect of auxin on cell wall glycanhydrolytic enzymes, which were extracted with LiCl. Our results show that incubation of sections of Cicer arietinum epicotyls with indoleacetic acid elicit some minor changes in electrophoretic patterns of cell wall proteins when compared with control sections. This indicates that there is no appearance of a specific polypeptide synthesized de novo in response to the hormone, although there are increases in the intensity of some of the polypeptides, which could indicate an enhancement of wall protein biosynthesis. Brief incubation with IAA led to a general increase in the specific activities of these different cell wall enzyme fractions separated by chromatography, with the exception of the α‐fraction, with α‐galactosidase activity. Longer incubation resulted in an increase in the amount of protein associated with some of the enzyme fractions. In particular, it induced a large increase in the amount of protein associated with the β111‐galactosidase fraction that is involved in the autolytic process of cell walls of chick‐pea epicotyls. Our results indicate that auxin‐enhanced growth could be the result of the action of the hormone al the level of the cell wall glycanhydrolytic proteins that have been related to the wall‐loosening process.
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Claims 7
Claim · model-written summary
Auxin caused minor shifts in wall protein electrophoretic patterns.
- Taxon
- Cicer arietinum
Cicer arietinume0 - Organ / stage
- epicotylse0
- Treatment
- indoleacetic acide0
- Outcome
- electrophoretic patterns of cell wall proteinse0
- Direction
- changese0
- Comparator
- control sectionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“incubation of sections of Cicer arietinum epicotyls with indoleacetic acid elicit some minor changes in electrophoretic patterns of cell wall proteins when compared with control sections.”
Claim · model-written summary
No specific polypeptide appeared de novo after auxin exposure.
- Taxon
- Cicer arietinumCicer arietinumt0
- Treatment
- the hormonee0
- Outcome
- no appearance of a specific polypeptide synthesized de novoe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is no appearance of a specific polypeptide synthesized de novo in response to the hormone”
t0“Cicer arietinum”
Claim · model-written summary
Some wall polypeptides increased in intensity after auxin.
- Taxon
- Cicer arietinumCicer arietinumt0
- Treatment
- auxint0
- Outcome
- intensity of some of the polypeptidese0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are increases in the intensity of some of the polypeptides, which could indicate an enhancement of wall protein biosynthesis.”
t0“auxin on cell wall glycanhydrolytic enzymes in epicotyls of Cicer arietinum”
Claim · model-written summary
Brief IAA incubation generally increased specific activities of cell wall enzyme fractions except the alpha fraction.
- Taxon
- Cicer arietinumCicer arietinumt0
- Treatment
- IAAe0
- Outcome
- specific activities of these different cell wall enzyme fractionse0
- Direction
- increasee0
- Qualifier
- with the exception of the α‐fraction, with α‐galactosidase activitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brief incubation with IAA led to a general increase in the specific activities of these different cell wall enzyme fractions separated by chromatography, with the exception of the α‐fraction, with α‐galactosidase activity.”
t0“Cicer arietinum”
Claim · model-written summary
Longer incubation increased protein amount in some enzyme fractions.
- Taxon
- Cicer arietinumCicer arietinumt0
- Treatment
- Longer incubatione0
- Outcome
- amount of protein associated with some of the enzyme fractionse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Longer incubation resulted in an increase in the amount of protein associated with some of the enzyme fractions.”
t0“Cicer arietinum”
Claim · model-written summary
Longer incubation greatly increased protein in the beta111 galactosidase fraction.
- Taxon
- chick‐peaCicer arietinume0
- Organ / stage
- epicotylse0
- Treatment
- auxint0
- Gene
- β111‐galactosidasee0
- Outcome
- amount of protein associated with the β111‐galactosidase fractione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it induced a large increase in the amount of protein associated with the β111‐galactosidase fraction that is involved in the autolytic process of cell walls of chick‐pea epicotyls.”
t0“auxin on cell wall glycanhydrolytic enzymes in epicotyls of Cicer arietinum”
Claim · model-written summary
Authors propose auxin-enhanced growth can arise from hormone effects on wall glycanhydrolytic proteins.
- Taxon
- Cicer arietinumCicer arietinumt0
- Outcome
- auxin‐enhanced growthe0
- Qualifier
- action of the hormone al the level of the cell wall glycanhydrolytic proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin‐enhanced growth could be the result of the action of the hormone al the level of the cell wall glycanhydrolytic proteins that have been related to the wall‐loosening process.”
t0“Cicer arietinum”
The same questions in other papers
indole-3-acetic acid · physiology: enzyme activity · Cicer arietinum (chickpea)Results agree
1 paper in the same direction as this paper
- positive
class · quality: protein · Cicer arietinum (chickpea)Results agree
2 papers in the same direction as this paper
- positive
- positive
class · molecular: metabolites · Cicer arietinum (chickpea)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.