Paper · 1992 · PLANT PHYSIOLOGY

Induction of Glutamine Synthetase Activity in Nonnodulated Roots of Glycine max, Phaseolus vulgaris, and Pisum sativum

Inger Hoelzle; John J. Finer; Michael D. McMullen; et al.

Read at the source ↗doi:10.1104/pp.100.1.525 ↗OpenAlex W2065167617 ↗In the paper network →

4
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4
verbatim quotes
18
citations
1
research areas
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Open access

Abstract

Nitrate or ammonium fertilization significantly increased glutamine synthetase (GS) activity in nonnodulated roots of French bean (Phaseolus vulgaris), soybean (Glycine max), and pea (Pisum sativum). Western analysis revealed substantial GS antibody-positive protein in root extracts that had minimal GS activity, indicating that an inactive form of GS may be present in nonfertilized plants.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Nitrate or ammonium fertilization significantly increased glutamine synthetase activity in French bean roots.

Taxon
French beanPhaseolus vulgarise0
Organ / stage
nonnodulated rootse0
Treatment
Nitrate or ammonium fertilizatione0
Outcome
glutamine synthetase (GS) activitye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Nitrate or ammonium fertilization significantly increased glutamine synthetase (GS) activity in nonnodulated roots of French bean (Phaseolus vulgaris)”

✓ located verbatim in the source · abstract, characters 0–150 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Nitrate or ammonium fertilization significantly increased glutamine synthetase activity in soybean roots.

Taxon
soybeanGlycine maxe0
Organ / stage
nonnodulated rootse0
Treatment
Nitrate or ammonium fertilizatione0
Outcome
glutamine synthetase (GS) activitye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“Nitrate or ammonium fertilization significantly increased glutamine synthetase (GS) activity in nonnodulated roots of French bean (Phaseolus vulgaris), soybean (Glycine max)”

✓ located verbatim in the source · abstract, characters 0–173 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Nitrate or ammonium fertilization significantly increased glutamine synthetase activity in pea roots.

Organ / stage
nonnodulated rootse0
Treatment
Nitrate or ammonium fertilizatione0
Outcome
glutamine synthetase (GS) activitye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Nitrate or ammonium fertilization significantly increased glutamine synthetase (GS) activity in nonnodulated roots of French bean (Phaseolus vulgaris), soybean (Glycine max), and pea (Pisum sativum)”

✓ located verbatim in the source · abstract, characters 0–198 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Root extracts with minimal glutamine synthetase activity contained substantial antibody-positive protein, suggesting inactive enzyme in nonfertilized plants.

Taxon
plantsViridiplantaee0
Organ / stage
root extractse0
Outcome
GS antibody-positive proteine0
Direction
substantiale0

Verbatim

e0

“Western analysis revealed substantial GS antibody-positive protein in root extracts that had minimal GS activity, indicating that an inactive form of GS may be present in nonfertilized plants.”

✓ located verbatim in the source · abstract, characters 200–392 · show in text ↑

nitrogen · molecular: metabolites · Phaseolus vulgaris (common bean)Results differ

11 papers: 3 positive, 8 negative or no effect

2 papers in the same direction as this paper · 8 in the opposite direction

  1. no effect
    In bean, one day of nitrate treatment caused minimal changes in carbon metabolites, antioxidants and other biochemical parameters.

    Stress-Induced Legume Root Nodule Senescence. Physiological, Biochemical, and Structural Alterations · 1999

    Opposite direction

  2. no effect
    In Phaseolus vulgaris, the qualitative pattern of organic acid components stayed unchanged under mineral deficiency, with few exceptions.

    Changes in the Organic Acid Content of some Cultivated Plants Induced by Mineral Ion Deficiency · 1981 · compared with full nutrient supply

    Opposite directionAbout lacking this nutrient, so it counts the other way round

  3. no effect
    The pattern of ureide distribution was not affected by nitrate or sucrose in the media up to 6 days after imbibition.

    Ureide metabolism during seedling development in French bean (Phaseolus vulgaris) · 2008

    Opposite direction

7 papers more in this group

See all 11 papers →

nitrogen · molecular: metabolites · Glycine max (soybean)Results differ

81 papers: 37 positive, 44 negative or no effect

36 papers in the same direction as this paper · 42 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
    Nitrate supply decreased ureides in soybean saps and extracts.

    Ureide Assay for Measuring Nitrogen Fixation by Nodulated Soybean Calibrated by 15N Methods · 1990

    Opposite direction

  2. no effect
    Altered nitrogen supply did not dramatically change relative amino acid profiles.

    Carbon and Nitrogen Provisions Alter the Metabolic Flux in Developing Soybean Embryos   · 2013

    Opposite direction

  3. negative
    High nitrate increased amino N in soybean nodules.

    Nitrate Inhibition of Legume Nodule Growth and Activity · 1985 · compared with control nodules

    Opposite direction

77 papers more in this group

See all 81 papers →

fertilization · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results

13 papers: 9 positive, 4 negative or no effect

8 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
    CSP increased phytic acid concentration in immature and mature pea seeds.

    Nutritional Quality of Vegetable Crops as Affected by Phosphorus and Zinc Fertilizers1 · 1980

    Opposite direction

  2. no effect
    Nitrogen fertilizer did not affect total oligosaccharide content.

    CARBOHYDRATE AND PROTEIN ACCUMULATION IN THE DEVELOPING FIELD PEA SEED · 1980

    Opposite direction

  3. negative
    Higher potassium doses significantly reduced seed nitrogen content.

    Effect of varied levels of fertilization with potassium on field pea yield and content and uptake of nitrogen. · 2017

    Opposite direction

9 papers more in this group

See all 13 papers →

nitrogen · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results

15 papers: 8 positive, 7 negative or no effect

7 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
    Nitrate application increased nitrite reductase activity in pea root plastids.

    Tissue and Cellular Distribution of Glutamine Synthetase in Roots of Pea (Pisum sativum) Seedlings · 1989 · compared with ammonium application

    Same directionCited by this paper

  2. no effect
    Net electron flow to silicomolybdate is insensitive to 3-(3,4-dichlorophenyl)-1,1-dimethyl urea.

    Characterisation of a PS II reaction centre isolated from the chloroplasts ofPisum sativum · 1987

    Opposite direction

  3. negative
    Ammonium-grown leaves had a considerably lower chloroplastic chloride concentration than nitrate-grown leaves at similar sodium and chloride contents.

    Replacement of nitrate by ammonium as the nitrogen source increases the salt sensitivity of pea plants. II. Inter‐ and intracellular solute compartmentation in leaflets · 1994 · compared with nitrate

    Opposite direction

11 papers more in this group

See all 15 papers →

fertilization · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results

21 papers: 18 positive, 3 negative or no effect

17 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
    CSP increased phytic acid concentration in mature snap bean seeds.

    Nutritional Quality of Vegetable Crops as Affected by Phosphorus and Zinc Fertilizers1 · 1980

    Opposite direction

  2. no effect
    NPS fertilizer did not affect soil cation exchange capacity.

    Optimum plant density and inorganic fertilizer application improved selected soil chemical properties and common bean productivity in southern Ethiopia · 2025

    Opposite direction

  3. no effect
    Microelement fertilisation did not change phosphorus, calcium and sodium contents in bean seeds.

    THE CONTENTS AND IONIC RATIOS OF THE SELECTED COMPONENTS OF BEAN IN THE BACKGROUND OF FERTILISATION WITH MICROELEMENTS · 2009

    Opposite direction

17 papers more in this group

See all 21 papers →

fertilization · molecular: metabolites · Glycine max (soybean)Positive and negative results

87 papers: 69 positive, 18 negative or no effect

68 papers in the same direction as this paper · 18 in the opposite direction

  1. no effect
    Phosphorus and K fertilization had a negligible effect on soybean tocopherol concentrations.

    Soybean Tocopherol Concentrations Are Affected by Crop Management · 2010

    Opposite direction

  2. no effect
    Stearic acid concentration was not influenced by P fertilization.

    Phosphorus Fertilization Differentially Influences Fatty Acids, Protein, and Oil in Soybean · 2016

    Opposite direction

  3. negative
    Increasing nutrient-solution Cd increased concentration and content in both plant parts.

    Absorption, accumulation and distribution of cadmium by two soybean CVS · 1994

    Opposite direction

83 papers more in this group

See all 87 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.