Abstract
The in vitro protein synthetic activity of 80S ribosomes from leaves of dark-grown corn seedlings was enhanced (at low Mg(2+) levels) by a 5-minute red light treatment applied 2 hours prior to tissue harvest. The effect was completely reversed by an immediate brief far red treatment, suggesting that ribosome activation is controlled by the phytochrome system. Experiments in which the interval between light treatment and tissue harvest was shortened indicate that the response was quite rapid. The initial increase in activity was detected within 30 minutes, followed by a rapid increase during the next 1.5 hours. No further increase occurred after 2 to 3 hours.The change in ribosome activity relates, at least in part, to an increase in the level of peptidyl-tRNA associated with ribosomes. Removal of peptidyl-tRNA from light-treated ribosomes also completely reversed the red light effect. Activation of ribosomes by 2 to 3 hours continuous white light (as previously reported) differs from red light activation in that reversal of this response requires salt washing of the ribosomes in addition to removal of peptidyl-tRNA.
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Claims 7
Claim · model-written summary
Red light enhanced protein synthetic activity of leaf ribosomes at low Mg(2+).
- Taxon
- corn
Zea mays subsp. mayse0 - Organ / stage
- leavese0
- Treatment
- 5-minute red light treatmente0
- Outcome
- protein synthetic activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in vitro protein synthetic activity of 80S ribosomes from leaves of dark-grown corn seedlings was enhanced (at low Mg(2+) levels) by a 5-minute red light treatment applied 2 hours prior to tissue harvest”
Claim · model-written summary
Immediate far red treatment reversed the red light effect.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect was completely reversed by an immediate brief far red treatment”
e1“red light treatment applied 2 hours prior to tissue harvest”
t0“Maize Ribosomes”
Claim · model-written summary
Ribosome activity first increased within 30 minutes and then rapidly over the next 1.5 hours.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The initial increase in activity was detected within 30 minutes, followed by a rapid increase during the next 1.5 hours”
t0“Maize Ribosomes”
Claim · model-written summary
Ribosome activity did not increase further after 2 to 3 hours.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No further increase occurred after 2 to 3 hours”
t0“Maize Ribosomes”
Claim · model-written summary
The change in activity was linked partly to increased peptidyl-tRNA associated with ribosomes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The change in ribosome activity relates, at least in part, to an increase in the level of peptidyl-tRNA associated with ribosomes”
t0“Maize Ribosomes”
Claim · model-written summary
Removing peptidyl-tRNA reversed the red light effect.
- Treatment
- Removal of peptidyl-tRNAe0
- Outcome
- red light effecte0
- Direction
- reversede0
- Comparator
- light-treated ribosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Removal of peptidyl-tRNA from light-treated ribosomes also completely reversed the red light effect”
t0“Maize Ribosomes”
Claim · model-written summary
Reversal of continuous white light activation required both salt washing and removal of peptidyl-tRNA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reversal of this response requires salt washing of the ribosomes in addition to removal of peptidyl-tRNA”
t0“Maize Ribosomes”
The same questions in other papers
light · quality: protein · Zea mays (maize)Positive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.