Abstract
Recent efforts have defined a cis-acting DNA regulatory element in plants, the C-repeat/dehydration responsive element (DRE), that stimulates transcription in response to low temperature and water deficit. Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat/DRE binding factor, CBF1 (C-repeat/DRE Binding Factor 1). Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain. CBF1 also has an AP2 domain, which is a DNA-binding motif of about 60 aa present in the Arabidopsis proteins APETALA2, AINTEGUMENTA, and TINY; the tobacco ethylene response element binding proteins; and numerous other plant proteins of unknown function. The transcript levels for CBF1, which appears to be a single or low copy number gene, did not change appreciably in plants exposed to low temperature or in detached leaves subjected to water deficit. Binding of CBF1 to the C-repeat/DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli. Moreover, expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat/DRE as an upstream activator sequence but not mutant versions of the DNA element. We conclude that CBF1 can function as a transcriptional activator that binds to the C-repeat/DRE DNA regulatory element and, thus, is likely to have a role in cold- and dehydration-regulated gene expression in Arabidopsis.
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Claims 8
Claim · model-written summary
An Arabidopsis thaliana cDNA encoding the C-repeat/DRE binding factor CBF1 was isolated.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Gene
- CBF1e0
- Outcome
- isolatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e0“Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat/DRE binding factor, CBF1”
Claim · model-written summary
CBF1 transcript levels did not change appreciably in plants exposed to low temperature or in detached leaves under water deficit.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- low temperature or in detached leaves subjected to water deficite1
- Gene
- CBF1e1
- Outcome
- transcript levels for CBF1e1
- Direction
- did not change appreciablye1
- Qualifier
- appears to be a single or low copy number genee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e1“The transcript levels for CBF1, which appears to be a single or low copy number gene, did not change appreciably in plants exposed to low temperature or in detached leaves subjected to water deficit”
Claim · model-written summary
CBF1 bound the C-repeat/DRE in gel shift assays with recombinant protein.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- CBF1e2
- Outcome
- Binding of CBF1 to the C-repeat/DREe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e2“Binding of CBF1 to the C-repeat/DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli”
Claim · model-written summary
Expression of CBF1 in yeast activated transcription of reporter genes carrying the C-repeat/DRE, but not mutant versions of the element.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- expression of CBF1 in yeaste3
- Gene
- CBF1e3
- Outcome
- transcription of reporter genese3
- Direction
- activatee3
- Comparator
- mutant versions of the DNA elemente3
- Setting
- yeaste3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e3“expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat/DRE as an upstream activator sequence but not mutant versions of the DNA element”
Claim · model-written summary
The deduced CBF1 protein has a molecular mass of 24 kDa.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- CBF1e4
- Outcome
- molecular masse4
- Value
- 24 kDae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e4“Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain”
Claim · model-written summary
The deduced CBF1 protein has a potential nuclear localization sequence and a possible acidic activation domain.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- CBF1e4
- Outcome
- potential nuclear localization sequencee4
- Qualifier
- possible acidic activation domaine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e4“Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain”
Claim · model-written summary
CBF1 has an AP2 DNA-binding domain.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- CBF1e5
- Outcome
- AP2 domaine5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e5“CBF1 also has an AP2 domain”
Claim · model-written summary
CBF1 is likely to have a role in cold- and dehydration-regulated gene expression in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- CBF1e6
- Outcome
- cold- and dehydration-regulated gene expressione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator”
e6“CBF1 can function as a transcriptional activator that binds to the C-repeat/DRE DNA regulatory element and, thus, is likely to have a role in cold- and dehydration-regulated gene expression in Arabidopsis”
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