Abstract
Cold, hyperosmolarity, and abscisic acid (ABA) signaling induce RD29A expression, which is an indicator of the plant stress adaptation response. Two nonallelic Arabidopsis thaliana (ecotype C24) T-DNA insertional mutations, cpl1 and cpl3, were identified based on hyperinduction of RD29A expression that was monitored by using the luciferase (LUC) reporter gene (RD29ALUC) imaging system. Genetic linkage analysis and complementation data established that the recessive cpl1 and cpl3 mutations are caused by T-DNA insertions in AtCPL1 (Arabidopsis C-terminal domain phosphatase-like) and AtCPL3, respectively. Gel assays using recombinant AtCPL1 and AtCPL3 detected innate phosphatase activity like other members of the phylogenetically conserved family that dephosphorylate the C-terminal domain of RNA polymerase II (RNAP II). cpl1 mutation causes RD29ALUC hyperexpression and transcript accumulation in response to cold, ABA, and NaCl treatments, whereas the cpl3 mutation mediates hyperresponsiveness only to ABA. Northern analysis confirmed that LUC transcript accumulation also occurs in response to these stimuli. cpl1 plants accumulate biomass more rapidly and exhibit delayed flowering relative to wild type whereas cpl3 plants grow more slowly and flower earlier than wild-type plants. Hence AtCPL1 and AtCPL3 are negative regulators of stress responsive gene transcription and modulators of growth and development. These results suggest that C-terminal domain phosphatase regulation of RNAP II phosphorylation status is a focal control point of complex processes like plant stress responses and development. AtCPL family members apparently have both unique and overlapping transcriptional regulatory functions that differentiate the signal output that determines the plant response.
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Claims 11
Claim · model-written summary
The cpl1 mutation was linked to a T-DNA insertion in AtCPL1.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- cpl1e0
- Gene
- AtCPL1e0
- Outcome
- T-DNA insertions in AtCPL1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recessive cpl1 and cpl3 mutations are caused by T-DNA insertions in AtCPL1 (Arabidopsis C-terminal domain phosphatase-like) and AtCPL3, respectively”
t0“Arabidopsis thaliana”
Claim · model-written summary
The cpl3 mutation was linked to a T-DNA insertion in AtCPL3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl3e0
- Gene
- AtCPL3e0
- Outcome
- T-DNA insertions in AtCPL1 (Arabidopsis C-terminal domain phosphatase-like) and AtCPL3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recessive cpl1 and cpl3 mutations are caused by T-DNA insertions in AtCPL1 (Arabidopsis C-terminal domain phosphatase-like) and AtCPL3, respectively”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtCPL1 had innate phosphatase activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtCPL1e0
- Outcome
- innate phosphatase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gel assays using recombinant AtCPL1 and AtCPL3 detected innate phosphatase activity”
t0“Arabidopsis thaliana”
Claim · model-written summary
AtCPL3 had innate phosphatase activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtCPL3e0
- Outcome
- innate phosphatase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gel assays using recombinant AtCPL1 and AtCPL3 detected innate phosphatase activity”
t0“Arabidopsis thaliana”
Claim · model-written summary
cpl1 hyperexpressed RD29ALUC under cold, ABA, and NaCl treatments.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl1e0
- Treatment
- cold, ABA, and NaCl treatmentse0
- Outcome
- RD29ALUC hyperexpression and transcript accumulatione0
- Direction
- hyperexpressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpl1 mutation causes RD29ALUC hyperexpression and transcript accumulation in response to cold, ABA, and NaCl treatments”
t0“Arabidopsis thaliana”
Claim · model-written summary
cpl3 was hyperresponsive only to ABA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl3e0
- Treatment
- ABAe0
- Outcome
- hyperresponsivenesse0
- Direction
- hyperresponsivenesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cpl3 mutation mediates hyperresponsiveness only to ABA”
t0“Arabidopsis thaliana”
Claim · model-written summary
LUC transcript accumulated in response to the stimuli.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- these stimulie0
- Outcome
- LUC transcript accumulatione0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LUC transcript accumulation also occurs in response to these stimuli”
t0“Arabidopsis thaliana”
Claim · model-written summary
cpl1 plants accumulated biomass more rapidly than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl1e0
- Outcome
- biomasse0
- Direction
- more rapidlye0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpl1 plants accumulate biomass more rapidly and exhibit delayed flowering relative to wild type”
t0“Arabidopsis thaliana”
Claim · model-written summary
cpl1 plants flowered later than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl1e0
- Outcome
- floweringe0
- Direction
- delayede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpl1 plants accumulate biomass more rapidly and exhibit delayed flowering relative to wild type”
t0“Arabidopsis thaliana”
Claim · model-written summary
cpl3 plants grew more slowly than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl3e0
- Outcome
- growe0
- Direction
- more slowlye0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpl3 plants grow more slowly and flower earlier than wild-type plants”
t0“Arabidopsis thaliana”
Claim · model-written summary
cpl3 plants flowered earlier than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cpl3e0
- Outcome
- flowere0
- Direction
- earliere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpl3 plants grow more slowly and flower earlier than wild-type plants”
t0“Arabidopsis thaliana”
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