Abstract
The isolation and characterization is reported of a cDNA for delta 1-pyrroline-5-carboxylate (P5C) synthetase (cAtP5CS), an enzyme involved in the biosynthesis of proline, from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana. Southern blot analysis suggested that only one copy of the corresponding gene (AtP5CS) is present in A. thaliana. The deduced amino acid sequence of the P5CS protein (AtP5CS) from A. thaliana exhibited 74% homology to that of the P5CS from Vigna aconitifolia. Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment. Moreover, the simultaneous accumulation of proline was observed as a result of the former treatments in A. thaliana. A cDNA for P5C reductase (cAtP5CR) was also isolated from A. thaliana and Northern blot analysis was performed. The AtP5CR gene was not induced to a significant extent by dehydration or high-salt stress. These observations suggest that the AtP5CS gene plays a principal role in the biosynthesis of proline in A. thaliana under osmotic stress.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Dehydration induced the P5CS gene in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- dehydratione1
- Gene
- P5CSe1
- Outcome
- the gene for P5CSe1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e1“Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment”
Claim · model-written summary
High salt induced the P5CS gene in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- high salte1
- Gene
- P5CSe1
- Outcome
- the gene for P5CSe1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e1“Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment”
Claim · model-written summary
ABA treatment induced the P5CS gene in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- ABAe1
- Gene
- P5CSe1
- Outcome
- the gene for P5CSe1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e1“Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment”
Claim · model-written summary
Heat or cold treatment did not induce the P5CS gene in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- heat or cold treatmente1
- Gene
- P5CSe1
- Outcome
- the gene for P5CSe1
- Direction
- not inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e1“Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment”
Claim · model-written summary
Proline accumulated simultaneously with the former treatments in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- former treatmentse2
- Outcome
- accumulation of prolinee2
- Direction
- simultaneous accumulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e2“Moreover, the simultaneous accumulation of proline was observed as a result of the former treatments in A. thaliana”
Claim · model-written summary
The AtP5CR gene was not induced to a significant extent by dehydration or high salt in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- dehydration or high-salt stresse3
- Gene
- AtP5CRe3
- Outcome
- AtP5CR genee3
- Direction
- not inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e3“The AtP5CR gene was not induced to a significant extent by dehydration or high-salt stress”
Claim · model-written summary
The deduced P5CS protein of Arabidopsis thaliana showed 74% homology to the P5CS of Vigna aconitifolia.
- Taxon
- A. thalianaArabidopsis thalianae4
- Gene
- P5CSe4
- Outcome
- deduced amino acid sequencee4
- Value
- 74%e4
- Comparator
- P5CS from Vigna aconitifoliae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana”
e4“The deduced amino acid sequence of the P5CS protein (AtP5CS) from A. thaliana exhibited 74% homology to that of the P5CS from Vigna aconitifolia”
Claim · model-written summary
Arabidopsis thaliana has only one copy of the AtP5CS gene.
- Taxon
- A. thalianaArabidopsis thalianae5
- Gene
- AtP5CSe5
- Outcome
- copy of the corresponding genee5
- Value
- one copye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Southern blot analysis suggested that only one copy of the corresponding gene (AtP5CS) is present in A. thaliana”
Claim · model-written summary
The AtP5CS gene plays a principal role in proline biosynthesis in Arabidopsis thaliana under osmotic stress.
- Taxon
- A. thalianaArabidopsis thalianae6
- Gene
- AtP5CSe6
- Outcome
- biosynthesis of prolinee6
- Qualifier
- under osmotic stresse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These observations suggest that the AtP5CS gene plays a principal role in the biosynthesis of proline in A. thaliana under osmotic stress”
The same questions in other papers
heat · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →drought · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
13 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
22 papers more in this group
See all 26 papers →cold · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
13 papers in the same direction as this paper · 19 in the opposite direction
- positive
- positive
- positive
29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.