Abstract
The dehydration-responsive element-binding factor 2C (DREB2C) is a member of the CBF/DREB subfamily of proteins, which contains a single APETALA2/Ethylene responsive element-binding factor (AP2/ERF) domain. To identify the expression pattern of the DREB2C gene, which contains multiple transcription cis-regulatory elements in its promoter, an approximately 1.4 kb upstream DREB2C sequence was fused to the β-glucuronidase reporter gene (GUS) and the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh. The promoter of the gene directed prominent GUS activity in the vasculature in diverse young dividing tissues. Upon applying heat stress (HS), GUS staining was also enhanced in the vasculature of the growing tissues. Analysis of a series of 5'-deletions of the DREB2C promoter revealed that a proximal upstream sequence sufficient for the tissue-specific spatial and temporal induction of GUS expression by HS is localized in the promoter region between -204 and -34 bps relative to the transcriptional start site. Furthermore, electrophoretic mobility shift assay (EMSA) demonstrated that nuclear protein binding activities specific to a -120 to -32 bp promoter fragment increased after HS. These results indicate that the TATA-proximal region and some latent trans-acting factors may cooperate in HS-induced activation of the Arabidopsis DREB2C promoter.
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Claims 5
Claim · model-written summary
The DREB2C promoter directed prominent GUS activity in vasculature of young dividing tissues.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Organ / stage
- vasculaturee0
- Gene
- DREB2Ct0
- Outcome
- GUS activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promoter of the gene directed prominent GUS activity in the vasculature in diverse young dividing tissues”
t0“Arabidopsis DREB2C”
e1“the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh”
Claim · model-written summary
Heat stress enhanced GUS staining in growing-tissue vasculature.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- vasculaturee0
- Treatment
- heat stress (HS)e0
- Gene
- DREB2Ct0
- Outcome
- GUS staininge0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon applying heat stress (HS), GUS staining was also enhanced in the vasculature of the growing tissues”
t0“Arabidopsis DREB2C”
e1“the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh”
Claim · model-written summary
The -204 to -34 bps promoter region was sufficient for tissue-specific heat induction of GUS expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HSe0
- Gene
- DREB2Ct0
- Outcome
- tissue-specific spatial and temporal induction of GUS expressione0
- Value
- -204 and -34 bpse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a proximal upstream sequence sufficient for the tissue-specific spatial and temporal induction of GUS expression by HS is localized in the promoter region between -204 and -34 bps relative to the transcriptional start site”
t0“Arabidopsis DREB2C”
e1“the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh”
Claim · model-written summary
Nuclear protein binding to the -120 to -32 bp promoter fragment increased after heat stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HSe0
- Gene
- DREB2Ct0
- Outcome
- nuclear protein binding activitiese0
- Direction
- increasede0
- Qualifier
- -120 to -32 bp promoter fragmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nuclear protein binding activities specific to a -120 to -32 bp promoter fragment increased after HS”
t0“Arabidopsis DREB2C”
e1“the recombinant p1244 construct was transformed into Arabidopsis thaliana (L.) Heynh”
Claim · model-written summary
The authors suggest TATA-proximal sequence and trans-acting factors cooperate in heat-induced DREB2C activation.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- DREB2Ce0
- Outcome
- HS-induced activation of the Arabidopsis DREB2C promotere0
- Qualifier
- TATA-proximal region and some latent trans-acting factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the TATA-proximal region and some latent trans-acting factors may cooperate in HS-induced activation of the Arabidopsis DREB2C promoter”
The same questions in other papers
heat · quality: protein · Arabidopsis thaliana (thale cress)Positive and negative results
2 in the opposite direction
- negative
- negative
heat · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
5 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- negative
- no effect
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.