Abstract
The establishment of three chlorophyllous callus phenotypes, Glycine max (L.) Merrill, cultured on a modified Miller's medium is described. Experiments were designed to determine the hormonal requirement necessary to maintain an adequate callus growth rate that would allow for the phenotypical accumulation of chlorophyll in all phenotypes. Addition of α ‐naphthaleneacetic acid and kinetin, both at 1 mg/liter, to the basal medium fulfilled this requirement. However, callus growth for these phenotypes required only an exogenous supply of cytokinin. All callus phenotypes, when maintained on 3% sucrose, were shown to possess similar growth curves; however, optimal growth rates of these cultured phenotypes occurred on different levels of exogenous sucrose (NG, 2%; LG, 1%; Y, 2%). Sucrose (filtered and autoclaved) and, in most cases, fructose (filtered), when employed as a carbon source in the basal medium, maintained adequate growth rates. Glucose (filtered) supported only minimal callus growth. These callus phenotypes, after two years in culture, showed a certain degree of cell type differentiation as indicated by the formation of isolated tracheoids and in some cases organized tracheoid development. The chromosome complement (2 n = 40) was observed to be polyploid.
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Claims 11
Claim · model-written summary
Adding auxin and kinetin met the callus growth requirement.
- Taxon
- Glycine max
Glycine maxe2 - Treatment
- α ‐naphthaleneacetic acid and kinetin, both at 1 mg/litere0
- Outcome
- adequate callus growth ratee1
- Comparator
- basal mediume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of α ‐naphthaleneacetic acid and kinetin, both at 1 mg/liter, to the basal medium fulfilled this requirement.”
e1“hormonal requirement necessary to maintain an adequate callus growth rate that would allow for the phenotypical accumulation of chlorophyll in all phenotypes.”
e2“Glycine max (L.) Merrill”
Claim · model-written summary
Callus growth required only exogenous cytokinin.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- exogenous supply of cytokinine0
- Outcome
- callus growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus growth for these phenotypes required only an exogenous supply of cytokinin.”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
All callus phenotypes had similar growth curves at 3% sucrose.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- 3% sucrosee0
- Outcome
- similar growth curvese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All callus phenotypes, when maintained on 3% sucrose, were shown to possess similar growth curves”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
NG callus grew optimally at 2% sucrose.
- Taxon
- Glycine maxGlycine maxe1
- Material
- NGe0
- Outcome
- optimal growth ratese0
- Value
- 2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimal growth rates of these cultured phenotypes occurred on different levels of exogenous sucrose (NG, 2%; LG, 1%; Y, 2%).”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
LG callus grew optimally at 1% sucrose.
- Taxon
- Glycine maxGlycine maxe1
- Material
- LGe0
- Outcome
- optimal growth ratese0
- Value
- 1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimal growth rates of these cultured phenotypes occurred on different levels of exogenous sucrose (NG, 2%; LG, 1%; Y, 2%).”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
Y callus grew optimally at 2% sucrose.
- Taxon
- Glycine maxGlycine maxe1
- Material
- Ye0
- Outcome
- optimal growth ratese0
- Value
- 2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimal growth rates of these cultured phenotypes occurred on different levels of exogenous sucrose (NG, 2%; LG, 1%; Y, 2%).”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
Sucrose maintained adequate callus growth.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Sucrose (filtered and autoclaved)e0
- Outcome
- adequate growth ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose (filtered and autoclaved) and, in most cases, fructose (filtered), when employed as a carbon source in the basal medium, maintained adequate growth rates.”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
Fructose usually maintained adequate callus growth.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- fructose (filtered)e0
- Outcome
- adequate growth ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose (filtered and autoclaved) and, in most cases, fructose (filtered), when employed as a carbon source in the basal medium, maintained adequate growth rates.”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
Glucose supported minimal callus growth.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Glucose (filtered)e0
- Outcome
- minimal callus growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glucose (filtered) supported only minimal callus growth.”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
Cultured callus formed isolated and sometimes organized tracheoids.
- Taxon
- Glycine maxGlycine maxe1
- Outcome
- formation of isolated tracheoids and in some cases organized tracheoid developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These callus phenotypes, after two years in culture, showed a certain degree of cell type differentiation as indicated by the formation of isolated tracheoids and in some cases organized tracheoid development.”
e1“Glycine max (L.) Merrill”
Claim · model-written summary
The chromosome complement was polyploid.
- Taxon
- Glycine maxGlycine maxe1
- Outcome
- chromosome complemente0
- Value
- 2 n = 40e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chromosome complement (2 n = 40) was observed to be polyploid.”
e1“Glycine max (L.) Merrill”
The same questions in other papers
kinetin · growth · Glycine max (soybean)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
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See all 10 papers →1-naphthaleneacetic acid · growth · Glycine max (soybean)Positive and negative results
14 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.