Abstract
The ATP-dependent proton-pumping activity of soybean (Glycine max L.) root microsomes is predominantly nitrate sensitive and presumably derived from the tonoplast. We used microsomes to characterize anion effects on proton pumping of the tonoplast vesicles using two distinctly different techniques.Preincubation of the vesicles with nitrate caused inhibition of proton pumping and ATPase activity, with similar concentration dependence. Fluoride, which preferentially inhibits the plasma membrane ATPase, inhibited ATPase activity strongly at concentrations which did not affect proton pumping activity.Addition of potassium salts, after a steady-state pH gradient is established in the absence of such salts, caused an increased pH gradient which was due to alleviation of Delta Psi and subsequent increased influx of H(+) into these vesicles. This anion-induced increase in the pH gradient could be used as a measure of the relative anion permeabilities, which were of the order Br(-) = NO(3) (-) > Cl(-) >> SO(4) (2-). Phosphate and fluoride caused no increase in the pH gradient. Since the concentration dependence of KCl- and KNO(3)-induced quenching exhibited a saturable component, and since H(+) uptake was increased by only certain anions, the data suggest that there may be a relatively specific anion channel associated with tonoplast-derived vesicles.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Soybean root microsome proton pumping was predominantly nitrate sensitive.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- root microsomese0
- Outcome
- ATP-dependent proton-pumping activitye0
- Direction
- nitrate sensitivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ATP-dependent proton-pumping activity of soybean (Glycine max L.) root microsomes is predominantly nitrate sensitive”
Claim · model-written summary
Nitrate preincubation inhibited proton pumping.
- Taxon
- soybeanGlycine maxt0
- Treatment
- nitratee0
- Outcome
- proton pumpinge0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preincubation of the vesicles with nitrate caused inhibition of proton pumping and ATPase activity, with similar concentration dependence”
t0“Soybean”
Claim · model-written summary
Nitrate preincubation inhibited ATPase activity.
- Taxon
- soybeanGlycine maxt0
- Treatment
- nitratee0
- Outcome
- ATPase activitye0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preincubation of the vesicles with nitrate caused inhibition of proton pumping and ATPase activity, with similar concentration dependence”
t0“Soybean”
Claim · model-written summary
Fluoride strongly inhibited ATPase activity at concentrations without proton-pumping effect.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Fluoridee0
- Outcome
- ATPase activitye0
- Direction
- inhibitede0
- Qualifier
- concentrations which did not affect proton pumping activitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluoride, which preferentially inhibits the plasma membrane ATPase, inhibited ATPase activity strongly at concentrations which did not affect proton pumping activity”
t0“Soybean”
Claim · model-written summary
Potassium salts increased the vesicle pH gradient.
- Taxon
- soybeanGlycine maxt0
- Treatment
- potassium saltse0
- Outcome
- pH gradiente0
- Direction
- increasede0
- Comparator
- absence of such saltse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of potassium salts, after a steady-state pH gradient is established in the absence of such salts, caused an increased pH gradient”
t0“Soybean”
Claim · model-written summary
Anion permeability ranked bromide and nitrate above chloride and sulfate.
- Taxon
- soybeanGlycine maxt0
- Outcome
- relative anion permeabilitiese0
- Direction
- Br(-) = NO(3) (-) > Cl(-) >> SO(4) (2-)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative anion permeabilities, which were of the order Br(-) = NO(3) (-) > Cl(-) >> SO(4) (2-)”
t0“Soybean”
Claim · model-written summary
Phosphate did not increase the pH gradient.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Phosphatee0
- Outcome
- pH gradiente0
- Direction
- no increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phosphate and fluoride caused no increase in the pH gradient”
t0“Soybean”
Claim · model-written summary
Fluoride did not increase the pH gradient.
- Taxon
- soybeanGlycine maxt0
- Treatment
- fluoridee0
- Outcome
- pH gradiente0
- Direction
- no increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phosphate and fluoride caused no increase in the pH gradient”
t0“Soybean”
Claim · model-written summary
KCl-induced quenching had a saturable component.
- Taxon
- soybeanGlycine maxt0
- Outcome
- KCl- and KNO(3)-induced quenchinge0
- Qualifier
- saturable componente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KCl- and KNO(3)-induced quenching exhibited a saturable component”
t0“Soybean”
Claim · model-written summary
Certain anions increased proton uptake.
- Taxon
- soybeanGlycine maxt0
- Treatment
- certain anionse0
- Outcome
- H(+) uptakee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(+) uptake was increased by only certain anions”
t0“Soybean”
Claim · model-written summary
The authors infer a relatively specific anion channel in tonoplast-derived vesicles.
- Taxon
- soybeanGlycine maxt0
- Outcome
- anion channele0
- Qualifier
- tonoplast-derived vesiclese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“data suggest that there may be a relatively specific anion channel associated with tonoplast-derived vesicles”
t0“Soybean”
The same questions in other papers
phosphorus · soil: chemistry · Glycine max (soybean)Positive and negative results
8 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.